{"id":196,"date":"2025-07-23T09:45:16","date_gmt":"2025-07-23T06:45:16","guid":{"rendered":"https:\/\/ckp.icgen.ru\/cells\/?page_id=196"},"modified":"2026-03-06T13:04:26","modified_gmt":"2026-03-06T10:04:26","slug":"icg_sb_ras_cell_lines_eng","status":"publish","type":"page","link":"https:\/\/ckp.icgen.ru\/cells\/icg_sb_ras_cell_lines_eng\/","title":{"rendered":"List of collection cell lines"},"content":{"rendered":"

Collective Center of ICG SB RAS «Collection of Pluripotent Human and Mammalian Cell cultures for Biological and Biomedical Research»<\/strong><\/p>\n

\u00a0<\/strong><\/p>\n

The collection contains embryonic stem and induced pluripotent stem cells of mice and other animals. There are also immortalized cell lines and protist cultures.<\/p>\n

Mouse embryonic stem cells can be used to produce transgenic animals.<\/p>\n

Unique cell lines of American mink pluripotent stem cells allow studying the early embryonic development of mustelids.<\/p>\n

 <\/p>\n

Services:<\/p>\n

— distribution of cell cultures;<\/p>\n

— training.<\/p>\n

Research areas:<\/p>\n

— derivation of cell lines for fundamental and applied research in the fields of developmental biology, cell biology and transgenesis, including embryonic stem and induced pluripotent stem cells of humans, mice and other laboratory animals, primary and with genetic modifications;<\/p>\n

— systematic description of the generated cell lines;<\/p>\n

— quality control of collection material using modern methods.<\/p>\n

 <\/p>\n

Manager:\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 Aleksei Gavriilovich Menzorov, Ph.D.<\/p>\n

Website:\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 http:\/\/ckp.icgen.ru\/cells\/<\/a><\/p>\n

E-mail: \u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 cellbank@bionet.nsc.ru<\/a><\/p>\n

 <\/p>\n

Contents<\/p>\n

Mouse pluripotent stem cells<\/a><\/p>\n

Cell line passport of DGES1<\/a><\/p>\n

Cell line passport of DGES2<\/a><\/p>\n

Cell line passport of DGES1-TubbEGFPpuro<\/a><\/p>\n

Cell line passport of DGES1-TubbEGFP<\/a><\/p>\n

Cell line passport of DGES1-TubbEGFPSV40puro<\/a><\/p>\n

Cell line passport of MA01<\/a><\/p>\n

Cell line passport of MA01-3E<\/a><\/p>\n

Cell line passport of MA02<\/a><\/p>\n

Cell line passport of MA03<\/a><\/p>\n

Cell line passport of MA04<\/a><\/p>\n

Cell line passport of MA05<\/a><\/p>\n

Cell line passport of MA06<\/a><\/p>\n

Cell line passport of MA07<\/a><\/p>\n

Cell line passport of MA08<\/a><\/p>\n

Cell line passport of MA09<\/a><\/p>\n

Cell line passport of MA10<\/a><\/p>\n

Cell line passport of MA11<\/a><\/p>\n

Cell line passport of MA12<\/a><\/p>\n

Cell line passport of MA13<\/a><\/p>\n

Cell line passport of MA15<\/a><\/p>\n

Cell line passport of MC01<\/a><\/p>\n

Cell line passport of MC02<\/a><\/p>\n

Cell line passport of MC03<\/a><\/p>\n

Cell line passport of MC04<\/a><\/p>\n

Cell line passport of MC05<\/a><\/p>\n

Cell line passport of MC06<\/a><\/p>\n

Cell line passport of MC07<\/a><\/p>\n

Cell line passport of MC08<\/a><\/p>\n

Cell line passport of MC09<\/a><\/p>\n

Cell line passport of MC10<\/a><\/p>\n

Cell line passport of MC11<\/a><\/p>\n

Cell line passport of MC12<\/a><\/p>\n

Cell line passport of MC13<\/a><\/p>\n

Cell line passport of MC15<\/a><\/p>\n

Cell line passport of MD01<\/a><\/p>\n

Cell line passport of MD02<\/a><\/p>\n

Hybrid cells<\/a><\/p>\n

Cell line passport of tme13<\/a><\/p>\n

Cell line passport of tme14<\/a><\/p>\n

Cell line passport of tme17<\/a><\/p>\n

Cell line passport of tmf1<\/a><\/p>\n

Cell line passport of tmf2<\/a><\/p>\n

Cell line passport of tmf5<\/a><\/p>\n

American mink pluripotent stem cells<\/a><\/p>\n

Cell line passport of MES12<\/a><\/p>\n

Cell line passport of MES20<\/a><\/p>\n

Cell line passport of MES22<\/a><\/p>\n

Cell line passport of MES24<\/a><\/p>\n

Cell line passport of MES25<\/a><\/p>\n

Cell line passport of MES27<\/a><\/p>\n

Cell line passport of MES29<\/a><\/p>\n

Cell line passport of iNV1XX1<\/a><\/p>\n

Cell line passport of iNV1XX2<\/a><\/p>\n

Cell line passport of iNV3<\/a><\/p>\n

Cell line passport of iNV5<\/a><\/p>\n

Cell line passport of iNV6<\/a><\/p>\n

Cell line passport of iNV7<\/a><\/p>\n

Cell line passport of iNV9<\/a><\/p>\n

Cell line passport of iNV11<\/a><\/p>\n

Cell line passport of iNV13<\/a><\/p>\n

Cell line passport of iNV15<\/a><\/p>\n

Cell line passport of iNV18<\/a><\/p>\n

Cell line passport of iNV19<\/a><\/p>\n

Cell line passport of iNV20<\/a><\/p>\n

Immortalised cell lines<\/a><\/p>\n

Cell line passport of CHO-hCNTN6-HA<\/a><\/p>\n

Cell line passport of CHO-hDLL1-HA<\/a><\/p>\n

Cell line passport of CHO-hNOTCH1-FLAG<\/a><\/p>\n

Cell line passport of CHO-hNOTCH2-FLAG<\/a><\/p>\n

Bird fibroblasts<\/a><\/p>\n

Cell line passport of OFC1A<\/a><\/p>\n

Protists<\/a><\/p>\n

Cell line passport of THAU1<\/a><\/p>\n

Cell line passport of THCA1<\/a><\/p>\n

Cell line passport of THKI1<\/a><\/p>\n

 <\/p>\n

 <\/p>\n

<\/a>Mouse pluripotent stem cells<\/h1>\n

<\/a>Cell line passport of DGES1<\/h2>\n

 <\/p>\n

Catalogue number:<\/strong> MMES00001<\/p>\n

Name:<\/strong> DGES1<\/p>\n

Description:<\/strong> mouse (Mus musculus<\/em>) embryonic stem cells derived from 129S2\/SvPasCrl 3.5D blastocyst<\/p>\n

Authors:<\/strong> Menzorov A.G., Matveeva N.M.<\/p>\n

Contamination analysis:<\/strong>\u00a0 bacteria, fungi and mycoplasma not detected<\/p>\n

Karyotype:<\/strong> 2n=40, XY, chromosome number variability 39-41, tetraploid cells 6%, modal chromosome number 40<\/p>\n

Pluripotency:<\/strong> pluripotency is shown by embrioid body formation, teratoma formation in SCID mice and germ line transmission<\/p>\n

Additional characteristics:<\/strong><\/p>\n

Species control:<\/strong> cytogenetic<\/p>\n

Cryoconservation passage:<\/strong> 6<\/p>\n

Area of application:<\/strong> transgenesis, developmental biology<\/p>\n

 <\/p>\n

Source<\/strong><\/p>\n

Species:<\/strong> Mus musculus<\/em><\/p>\n

Tissue:<\/strong> blastocyst<\/p>\n

Date:<\/strong> 01.01.2016<\/p>\n

 <\/p>\n

Cell culture<\/strong><\/p>\n

Morphology:<\/strong> mouse pluripotent stem cell colonies<\/p>\n

Cell culture method:<\/strong> monolayer<\/p>\n

Cell culture medium:<\/strong> DMEM (glucose 4.5 g\/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U\/ml<\/p>\n

Cell culture conditions:<\/strong> 37\u00b0C, 5% CO2<\/sub><\/p>\n

Passage protocol:<\/strong> passage with trypsin-EDTA 0.05%, split 1:3 — 1:6<\/p>\n

Cryoconservation:<\/strong> 90% KSR, 10% DMSO<\/p>\n

Cryoconservation cell concentration:<\/strong> 0.5 mln cells \/ ml<\/p>\n

Cell viability after cryoconservation:<\/strong> 70%<\/p>\n

Additional information:<\/strong> Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.<\/p>\n

\u00a0<\/strong><\/p>\n

References:<\/strong> https:\/\/doi.org\/10.1002\/jcb.28981<\/a><\/p>\n

<\/a>Cell line passport of DGES2<\/h2>\n

 <\/p>\n

Catalogue number:<\/strong> MMES00002<\/p>\n

Name:<\/strong> DGES2<\/p>\n

Description:<\/strong> mouse (Mus musculus<\/em>) embryonic stem cells derived from 129S2\/SvPasCrl 3.5D blastocyst<\/p>\n

Authors:<\/strong> Menzorov A.G., Matveeva N.M.<\/p>\n

Contamination analysis:<\/strong> bacteria, fungi and mycoplasma not detected<\/p>\n

Karyotype:<\/strong> 2n=40, XY, chromosome number variability 39-41, tetraploid cells 5%, modal chromosome number 40<\/p>\n

Pluripotency:<\/strong> pluripotency is shown by embrioid body formation, teratoma formation in SCID mice and germ line transmission<\/p>\n

Additional characteristics:<\/strong><\/p>\n

Species control:<\/strong> cytogenetic<\/p>\n

Cryoconservation passage:<\/strong> 6<\/p>\n

Area of application:<\/strong> transgenesis, developmental biology<\/p>\n

 <\/p>\n

Source<\/strong><\/p>\n

Species:<\/strong> Mus musculus<\/em><\/p>\n

Tissue:<\/strong> blastocyst<\/p>\n

Date:<\/strong> 01.01.2016<\/p>\n

 <\/p>\n

Cell culture<\/strong><\/p>\n

Morphology:<\/strong> mouse pluripotent stem cell colonies<\/p>\n

Cell culture method:<\/strong> monolayer<\/p>\n

Cell culture medium:<\/strong> DMEM (glucose 4.5 g\/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U\/ml<\/p>\n

Cell culture conditions:<\/strong> 37\u00b0C, 5% CO2<\/sub><\/p>\n

Passage protocol:<\/strong> passage with trypsin-EDTA 0.05%, split 1:3 — 1:6<\/p>\n

Cryoconservation:<\/strong> 90% KSR, 10% DMSO<\/p>\n

Cryoconservation cell concentration:<\/strong> 0.5 mln cells \/ ml<\/p>\n

Cell viability after cryoconservation:<\/strong> 70%<\/p>\n

Additional information:<\/strong> Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.<\/p>\n

\u00a0<\/strong><\/p>\n

References:<\/strong> https:\/\/doi.org\/10.1002\/jcb.28981<\/a><\/p>\n

<\/a>Cell line passport of DGES1-TubbEGFPpuro<\/h2>\n

 <\/p>\n

Catalogue number:<\/strong> MMES00038<\/p>\n

Name:<\/strong> DGES1-TubbEGFPpuro<\/p>\n

Description:<\/strong> DGES1 mouse embryonic stem cells with site-specific EGFP insertion after bTubb3 last exon via 2A peptide and puromycin resistance<\/p>\n

Authors:<\/strong> Menzorov A.G.<\/p>\n

Contamination analysis:<\/strong> bacteria, fungi and mycoplasma not detected<\/p>\n

Karyotype:<\/strong> 2n=40, XY, chromosome number variability 39-41, tetraploid cells less than 10%, modal chromosome number 40<\/p>\n

Pluripotency:<\/strong> pluripotency is shown by expression of specific markers<\/p>\n

Additional characteristics:<\/strong><\/p>\n

Species control:<\/strong> cytogenetic<\/p>\n

Cryoconservation passage:<\/strong> 24<\/p>\n

Area of application:<\/strong> transgenesis, developmental biology<\/p>\n

 <\/p>\n

Source<\/strong><\/p>\n

Species:<\/strong> Mus musculus<\/em><\/p>\n

Tissue:<\/strong><\/p>\n

Date:<\/strong> 01.01.2017<\/p>\n

 <\/p>\n

Cell culture<\/strong><\/p>\n

Morphology:<\/strong> mouse pluripotent stem cell colonies<\/p>\n

Cell culture method:<\/strong> monolayer<\/p>\n

Cell culture medium:<\/strong> DMEM (glucose 4.5 g\/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U\/ml<\/p>\n

Cell culture conditions:<\/strong> 37\u00b0C, 5% CO2<\/sub><\/p>\n

Passage protocol:<\/strong> passage with trypsin-EDTA 0.05%, split 1:3 — 1:6<\/p>\n

Cryoconservation:<\/strong> 90% KSR, 10% DMSO<\/p>\n

Cryoconservation cell concentration:<\/strong> 0.5 mln cells \/ ml<\/p>\n

Cell viability after cryoconservation:<\/strong> 70%<\/p>\n

Additional information:<\/strong> Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.<\/p>\n

\u00a0<\/strong><\/p>\n

References:<\/strong> https:\/\/doi.org\/10.1002\/jcb.28981<\/a><\/p>\n

<\/a>Cell line passport of DGES1-TubbEGFP<\/h2>\n

 <\/p>\n

Catalogue number:<\/strong> MMES00039<\/p>\n

Name:<\/strong> DGES1-TubbEGFP<\/p>\n

Description:<\/strong> DGES1 mouse embryonic stem cells with site-specific EGFP insertion after bTubb3 last exon via 2A peptide<\/p>\n

Authors:<\/strong> Menzorov A.G.<\/p>\n

Contamination analysis:<\/strong> bacteria, fungi and mycoplasma not detected<\/p>\n

Karyotype:<\/strong> 2n=40, XY, chromosome number variability 39-41, tetraploid cells less than 10%, modal chromosome number 40<\/p>\n

Pluripotency:<\/strong> pluripotency is shown by expression of specific markers<\/p>\n

Additional characteristics:<\/strong><\/p>\n

Species control:<\/strong> cytogenetic<\/p>\n

Cryoconservation passage:<\/strong> 22<\/p>\n

Area of application:<\/strong> transgenesis, developmental biology<\/p>\n

 <\/p>\n

Source<\/strong><\/p>\n

Species:<\/strong> Mus musculus<\/em><\/p>\n

Tissue:<\/strong><\/p>\n

Date:<\/strong> 01.01.2017<\/p>\n

 <\/p>\n

Cell culture<\/strong><\/p>\n

Morphology:<\/strong> mouse pluripotent stem cell colonies<\/p>\n

Cell culture method:<\/strong> monolayer<\/p>\n

Cell culture medium:<\/strong> DMEM (glucose 4.5 g\/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U\/ml<\/p>\n

Cell culture conditions:<\/strong> 37\u00b0C, 5% CO2<\/sub><\/p>\n

Passage protocol:<\/strong> passage with trypsin-EDTA 0.05%, split 1:3 — 1:6<\/p>\n

Cryoconservation:<\/strong> 90% KSR, 10% DMSO<\/p>\n

Cryoconservation cell concentration:<\/strong> 0.5 mln cells \/ ml<\/p>\n

Cell viability after cryoconservation:<\/strong> 70%<\/p>\n

Additional information:<\/strong> Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.<\/p>\n

\u00a0<\/strong><\/p>\n

References:<\/strong> https:\/\/doi.org\/10.1002\/jcb.28981<\/a><\/p>\n

<\/a>Cell line passport of DGES1-TubbEGFPSV40puro<\/h2>\n

 <\/p>\n

Catalogue number:<\/strong> MMES00040<\/p>\n

Name:<\/strong> DGES1-TubbEGFPSV40puro<\/p>\n

Description:<\/strong> DGES1 mouse embryonic stem cells with site-specific EGFP insertion after bTubb3 last exon via 2A peptide, SV40 polyA signal and puromycin resistance (site-specific and unspecific insert)<\/p>\n

Authors:<\/strong> Menzorov A.G.<\/p>\n

Contamination analysis:<\/strong> bacteria, fungi and mycoplasma not detected<\/p>\n

Karyotype:<\/strong> 2n=40, XY, chromosome number variability 39-41, tetraploid cells less than 5%, modal chromosome number 40<\/p>\n

Pluripotency:<\/strong> pluripotency is shown by expression of specific markers<\/p>\n

Additional characteristics:<\/strong><\/p>\n

Species control:<\/strong> cytogenetic<\/p>\n

Cryoconservation passage:<\/strong> 20<\/p>\n

Area of application:<\/strong> transgenesis, developmental biology<\/p>\n

 <\/p>\n

Source<\/strong><\/p>\n

Species:<\/strong> Mus musculus<\/em><\/p>\n

Tissue:<\/strong><\/p>\n

Date:<\/strong> 01.01.2017<\/p>\n

 <\/p>\n

Cell culture<\/strong><\/p>\n

Morphology:<\/strong> mouse pluripotent stem cell colonies<\/p>\n

Cell culture method:<\/strong> monolayer<\/p>\n

Cell culture medium:<\/strong> DMEM (glucose 4.5 g\/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U\/ml<\/p>\n

Cell culture conditions:<\/strong> 37\u00b0C, 5% CO2<\/sub><\/p>\n

Passage protocol:<\/strong> passage with trypsin-EDTA 0.05%, split 1:3 — 1:6<\/p>\n

Cryoconservation:<\/strong> 90% KSR, 10% DMSO<\/p>\n

Cryoconservation cell concentration:<\/strong> 0.5 mln cells \/ ml<\/p>\n

Cell viability after cryoconservation:<\/strong> 70%<\/p>\n

Additional information:<\/strong> Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.<\/p>\n

\u00a0<\/strong><\/p>\n

References:<\/strong> https:\/\/doi.org\/10.1002\/jcb.28981<\/a>
\nCell line passport of DGES1-TubbEGFPSV40<\/p>\n

 <\/p>\n

Catalogue number:<\/strong> MMES00041<\/p>\n

Name:<\/strong> DGES1-TubbEGFPSV40<\/p>\n

Description:<\/strong> DGES1 mouse embryonic stem cells with site-specific EGFP insertion after bTubb3 last exon via 2A peptide, SV40 polyA signal and puromycin resistance (unspecific insert)<\/p>\n

Authors:<\/strong> Menzorov A.G.<\/p>\n

Contamination analysis:<\/strong> bacteria, fungi and mycoplasma not detected<\/p>\n

Karyotype:<\/strong> 2n=40, XY, chromosome number variability 39-40, tetraploid cells less than 2%, modal chromosome number 40<\/p>\n

Pluripotency:<\/strong> pluripotency is shown by expression of specific markers<\/p>\n

Additional characteristics:<\/strong><\/p>\n

Species control:<\/strong> cytogenetic<\/p>\n

Cryoconservation passage:<\/strong> 26<\/p>\n

Area of application:<\/strong> transgenesis, developmental biology<\/p>\n

 <\/p>\n

Source<\/strong><\/p>\n

Species:<\/strong> Mus musculus<\/em><\/p>\n

Tissue:<\/strong><\/p>\n

Date:<\/strong> 01.01.2017<\/p>\n

 <\/p>\n

Cell culture<\/strong><\/p>\n

Morphology:<\/strong> mouse pluripotent stem cell colonies<\/p>\n

Cell culture method:<\/strong> monolayer<\/p>\n

Cell culture medium:<\/strong> DMEM (glucose 4.5 g\/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U\/ml<\/p>\n

Cell culture conditions:<\/strong> 37\u00b0C, 5% CO2<\/sub><\/p>\n

Passage protocol:<\/strong> passage with trypsin-EDTA 0.05%, split 1:3 — 1:6<\/p>\n

Cryoconservation:<\/strong> 90% KSR, 10% DMSO<\/p>\n

Cryoconservation cell concentration:<\/strong> 0.5 mln cells \/ ml<\/p>\n

Cell viability after cryoconservation:<\/strong> 70%<\/p>\n

Additional information:<\/strong> Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.<\/p>\n

\u00a0<\/strong><\/p>\n

References:<\/strong> https:\/\/doi.org\/10.1002\/jcb.28981<\/a><\/p>\n

<\/a>Cell line passport of MA01<\/h2>\n

 <\/p>\n

Catalogue number:<\/strong> MMES00004<\/p>\n

Name:<\/strong> MA01<\/p>\n

Description:<\/strong> mouse (Mus musculus<\/em>) embryonic stem cells derived from outbred BALB x 129\/Ola blastocyst<\/p>\n

Authors:<\/strong> Menzorov A.G.<\/p>\n

Contamination analysis:<\/strong> bacteria, fungi and mycoplasma not detected<\/p>\n

Karyotype:<\/strong> 2n=40, XY, chromosome number variability 39-45, tetraploid cells less than 2%, modal chromosome number 40<\/p>\n

Pluripotency:<\/strong> pluripotency is shown by teratoma formation in NU\/NU mice and germ line transmission<\/p>\n

Additional characteristics:<\/strong><\/p>\n

Species control:<\/strong> cytogenetic<\/p>\n

Cryoconservation passage:<\/strong> 4<\/p>\n

Area of application:<\/strong> transgenesis, developmental biology<\/p>\n

 <\/p>\n

Source<\/strong><\/p>\n

Species:<\/strong> Mus musculus<\/em><\/p>\n

Tissue:<\/strong> blastocyst<\/p>\n

Date:<\/strong> 01.01.2016<\/p>\n

 <\/p>\n

Cell culture<\/strong><\/p>\n

Morphology:<\/strong> mouse pluripotent stem cell colonies<\/p>\n

Cell culture method:<\/strong> monolayer<\/p>\n

Cell culture medium:<\/strong> DMEM (glucose 4.5 g\/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U\/ml<\/p>\n

Cell culture conditions:<\/strong> 37\u00b0C, 5% CO2<\/sub><\/p>\n

Passage protocol:<\/strong> passage with trypsin-EDTA 0.05%, split 1:3 — 1:6<\/p>\n

Cryoconservation:<\/strong> 90% KSR, 10% DMSO<\/p>\n

Cryoconservation cell concentration:<\/strong> 0.5 mln cells \/ ml<\/p>\n

Cell viability after cryoconservation:<\/strong> 70%<\/p>\n

Additional information:<\/strong> Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.<\/p>\n

\u00a0<\/strong><\/p>\n

References:<\/strong> https:\/\/doi.org\/10.1007\/s10616-014-9751-y<\/a><\/p>\n

<\/a>Cell line passport of MA01-3E<\/h2>\n

 <\/p>\n

Catalogue number:<\/strong> MMES00037<\/p>\n

Name:<\/strong> MA01-3E<\/p>\n

Description:<\/strong> MA01 mouse embryonic stem cells with EGFP cassette in Trim71 gene<\/p>\n

Authors:<\/strong> Menzorov A.G.<\/p>\n

Contamination analysis:<\/strong> bacteria, fungi and mycoplasma not detected<\/p>\n

Karyotype:<\/strong> 2n=40, XY, chromosome number variability 39-41, tetraploid cells less than 2%, modal chromosome number 40<\/p>\n

Pluripotency:<\/strong> pluripotency is shown by teratoma formation in SCID mice and germ line transmission<\/p>\n

Additional characteristics:<\/strong><\/p>\n

Species control:<\/strong> cytogenetic<\/p>\n

Cryoconservation passage:<\/strong> 15<\/p>\n

Area of application:<\/strong> transgenesis, developmental biology<\/p>\n

 <\/p>\n

Source<\/strong><\/p>\n

Species:<\/strong> Mus musculus<\/em><\/p>\n

Tissue:<\/strong><\/p>\n

Date:<\/strong> 01.01.2017<\/p>\n

 <\/p>\n

Cell culture<\/strong><\/p>\n

Morphology:<\/strong> mouse pluripotent stem cell colonies<\/p>\n

Cell culture method:<\/strong> monolayer<\/p>\n

Cell culture medium:<\/strong> DMEM (glucose 4.5 g\/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U\/ml<\/p>\n

Cell culture conditions:<\/strong> 37\u00b0C, 5% CO2<\/sub><\/p>\n

Passage protocol:<\/strong> passage with trypsin-EDTA 0.05%, split 1:3 — 1:6<\/p>\n

Cryoconservation:<\/strong> 90% KSR, 10% DMSO<\/p>\n

Cryoconservation cell concentration:<\/strong> 0.5 mln cells \/ ml<\/p>\n

Cell viability after cryoconservation:<\/strong> 70%<\/p>\n

Additional information:<\/strong> Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.<\/p>\n

\u00a0<\/strong><\/p>\n

References:<\/strong> https:\/\/doi.org\/10.1007\/s10616-014-9751-y<\/a><\/p>\n

<\/a>Cell line passport of MA02<\/h2>\n

 <\/p>\n

Catalogue number:<\/strong> MMES00005<\/p>\n

Name:<\/strong> MA02<\/p>\n

Description:<\/strong> mouse (Mus musculus<\/em>) embryonic stem cells derived from outbred BALB x 129\/Ola blastocyst<\/p>\n

Authors:<\/strong> Menzorov A.G.<\/p>\n

Contamination analysis:<\/strong> bacteria, fungi and mycoplasma not detected<\/p>\n

Karyotype:<\/strong> 2n=40, XX, chromosome number variability 39-41, tetraploid cells 13%, modal chromosome number 40<\/p>\n

Pluripotency:<\/strong> pluripotency is shown by teratoma formation in NU\/NU mice and chimeric animal generation<\/p>\n

Additional characteristics:<\/strong><\/p>\n

Species control:<\/strong> cytogenetic<\/p>\n

Cryoconservation passage:<\/strong> 4<\/p>\n

Area of application:<\/strong> transgenesis, developmental biology<\/p>\n

 <\/p>\n

Source<\/strong><\/p>\n

Species:<\/strong> Mus musculus<\/em><\/p>\n

Tissue:<\/strong> blastocyst<\/p>\n

Date:<\/strong> 01.01.2016<\/p>\n

 <\/p>\n

Cell culture<\/strong><\/p>\n

Morphology:<\/strong> mouse pluripotent stem cell colonies<\/p>\n

Cell culture method:<\/strong> monolayer<\/p>\n

Cell culture medium:<\/strong> DMEM (glucose 4.5 g\/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U\/ml<\/p>\n

Cell culture conditions:<\/strong> 37\u00b0C, 5% CO2<\/sub><\/p>\n

Passage protocol:<\/strong> passage with trypsin-EDTA 0.05%, split 1:3 — 1:6<\/p>\n

Cryoconservation:<\/strong> 90% KSR, 10% DMSO<\/p>\n

Cryoconservation cell concentration:<\/strong> 0.5 mln cells \/ ml<\/p>\n

Cell viability after cryoconservation:<\/strong> 70%<\/p>\n

Additional information:<\/strong> Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.<\/p>\n

\u00a0<\/strong><\/p>\n

References:<\/strong> https:\/\/doi.org\/10.1007\/s10616-014-9751-y<\/a><\/p>\n

<\/a>Cell line passport of MA03<\/h2>\n

 <\/p>\n

Catalogue number:<\/strong> MMES00006<\/p>\n

Name:<\/strong> MA03<\/p>\n

Description:<\/strong> mouse (Mus musculus<\/em>) embryonic stem cells derived from outbred BALB x 129\/Ola blastocyst<\/p>\n

Authors:<\/strong> Menzorov A.G.<\/p>\n

Contamination analysis:<\/strong> bacteria, fungi and mycoplasma not detected<\/p>\n

Karyotype:<\/strong> 2n=40, XX, chromosome number variability 40-42, 71-83, tetraploid cells 79%, modal chromosome number 78, 79<\/p>\n

Pluripotency:<\/strong> pluripotency is shown by expression of specific markers<\/p>\n

Additional characteristics:<\/strong><\/p>\n

Species control:<\/strong> cytogenetic<\/p>\n

Cryoconservation passage:<\/strong> 7<\/p>\n

Area of application:<\/strong> transgenesis, developmental biology<\/p>\n

 <\/p>\n

Source<\/strong><\/p>\n

Species:<\/strong> Mus musculus<\/em><\/p>\n

Tissue:<\/strong> blastocyst<\/p>\n

Date:<\/strong> 01.01.2016<\/p>\n

 <\/p>\n

Cell culture<\/strong><\/p>\n

Morphology:<\/strong> mouse pluripotent stem cell colonies<\/p>\n

Cell culture method:<\/strong> monolayer<\/p>\n

Cell culture medium:<\/strong> DMEM (glucose 4.5 g\/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U\/ml<\/p>\n

Cell culture conditions:<\/strong> 37\u00b0C, 5% CO2<\/sub><\/p>\n

Passage protocol:<\/strong> passage with trypsin-EDTA 0.05%, split 1:3 — 1:6<\/p>\n

Cryoconservation:<\/strong> 90% KSR, 10% DMSO<\/p>\n

Cryoconservation cell concentration:<\/strong> 0.5 mln cells \/ ml<\/p>\n

Cell viability after cryoconservation:<\/strong> 70%<\/p>\n

Additional information:<\/strong> Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.<\/p>\n

\u00a0<\/strong><\/p>\n

References:<\/strong> https:\/\/doi.org\/10.1007\/s10616-014-9751-y<\/a><\/p>\n

<\/a>Cell line passport of MA04<\/h2>\n

 <\/p>\n

Catalogue number:<\/strong> MMES00007<\/p>\n

Name:<\/strong> MA04<\/p>\n

Description:<\/strong> mouse (Mus musculus<\/em>) embryonic stem cells derived from outbred BALB x 129\/Ola blastocyst<\/p>\n

Authors:<\/strong> Menzorov A.G.<\/p>\n

Contamination analysis:<\/strong> bacteria, fungi and mycoplasma not detected<\/p>\n

Karyotype:<\/strong> 2n=40, XX, chromosome number variability 39-42, tetraploid cells 28%, modal chromosome number 40<\/p>\n

Pluripotency:<\/strong> pluripotency is shown by teratoma formation in NU\/NU mice<\/p>\n

Additional characteristics:<\/strong><\/p>\n

Species control:<\/strong> cytogenetic<\/p>\n

Cryoconservation passage:<\/strong> 4<\/p>\n

Area of application:<\/strong> transgenesis, developmental biology<\/p>\n

 <\/p>\n

Source<\/strong><\/p>\n

Species:<\/strong> Mus musculus<\/em><\/p>\n

Tissue:<\/strong> blastocyst<\/p>\n

Date:<\/strong> 01.01.2016<\/p>\n

 <\/p>\n

Cell culture<\/strong><\/p>\n

Morphology:<\/strong> mouse pluripotent stem cell colonies<\/p>\n

Cell culture method:<\/strong> monolayer<\/p>\n

Cell culture medium:<\/strong> DMEM (glucose 4.5 g\/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U\/ml<\/p>\n

Cell culture conditions:<\/strong> 37\u00b0C, 5% CO2<\/sub><\/p>\n

Passage protocol:<\/strong> passage with trypsin-EDTA 0.05%, split 1:3 — 1:6<\/p>\n

Cryoconservation:<\/strong> 90% KSR, 10% DMSO<\/p>\n

Cryoconservation cell concentration:<\/strong> 0.5 mln cells \/ ml<\/p>\n

Cell viability after cryoconservation:<\/strong> 70%<\/p>\n

Additional information:<\/strong> Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.<\/p>\n

\u00a0<\/strong><\/p>\n

References:<\/strong> https:\/\/doi.org\/10.1007\/s10616-014-9751-y<\/a><\/p>\n

 <\/p>\n

<\/a>Cell line passport of MA05<\/h2>\n

 <\/p>\n

Catalogue number:<\/strong> MMES00008<\/p>\n

Name:<\/strong> MA05<\/p>\n

Description:<\/strong> mouse (Mus musculus<\/em>) embryonic stem cells derived from outbred BALB x 129\/Ola blastocyst<\/p>\n

Authors:<\/strong> Menzorov A.G.<\/p>\n

Contamination analysis:<\/strong> bacteria, fungi and mycoplasma not detected<\/p>\n

Karyotype:<\/strong> 2n=40, XY, chromosome number variability 39-42, tetraploid cells 18%, modal chromosome number 40<\/p>\n

Pluripotency:<\/strong> pluripotency is shown by expression of specific markers<\/p>\n

Additional characteristics:<\/strong><\/p>\n

Species control:<\/strong> cytogenetic<\/p>\n

Cryoconservation passage:<\/strong> 8<\/p>\n

Area of application:<\/strong> transgenesis, developmental biology<\/p>\n

 <\/p>\n

Source<\/strong><\/p>\n

Species:<\/strong> Mus musculus<\/em><\/p>\n

Tissue:<\/strong> blastocyst<\/p>\n

Date:<\/strong> 01.01.2016<\/p>\n

 <\/p>\n

Cell culture<\/strong><\/p>\n

Morphology:<\/strong> mouse pluripotent stem cell colonies<\/p>\n

Cell culture method:<\/strong> monolayer<\/p>\n

Cell culture medium:<\/strong> DMEM (glucose 4.5 g\/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U\/ml<\/p>\n

Cell culture conditions:<\/strong> 37\u00b0C, 5% CO2<\/sub><\/p>\n

Passage protocol:<\/strong> passage with trypsin-EDTA 0.05%, split 1:3 — 1:6<\/p>\n

Cryoconservation:<\/strong> 90% KSR, 10% DMSO<\/p>\n

Cryoconservation cell concentration:<\/strong> 0.5 mln cells \/ ml<\/p>\n

Cell viability after cryoconservation:<\/strong> 70%<\/p>\n

Additional information:<\/strong> Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.<\/p>\n

\u00a0<\/strong><\/p>\n

References:<\/strong> https:\/\/doi.org\/10.1007\/s10616-014-9751-y<\/a><\/p>\n

<\/a>Cell line passport of MA06<\/h2>\n

 <\/p>\n

Catalogue number:<\/strong> MMES00009<\/p>\n

Name:<\/strong> MA06<\/p>\n

Description:<\/strong> mouse (Mus musculus<\/em>) embryonic stem cells derived from outbred BALB x 129\/Ola blastocyst<\/p>\n

Authors:<\/strong> Menzorov A.G.<\/p>\n

Contamination analysis:<\/strong> bacteria, fungi and mycoplasma not detected<\/p>\n

Karyotype:<\/strong> 2n=40, XX, chromosome number variability 40-41, tetraploid cells 17%, modal chromosome number 40<\/p>\n

Pluripotency:<\/strong> pluripotency is shown by teratoma formation in NU\/NU mice and chimeric animal generation<\/p>\n

Additional characteristics:<\/strong><\/p>\n

Species control:<\/strong> cytogenetic<\/p>\n

Cryoconservation passage:<\/strong> 4<\/p>\n

Area of application:<\/strong> transgenesis, developmental biology<\/p>\n

 <\/p>\n

Source<\/strong><\/p>\n

Species:<\/strong> Mus musculus<\/em><\/p>\n

Tissue:<\/strong> blastocyst<\/p>\n

Date:<\/strong> 01.01.2016<\/p>\n

 <\/p>\n

Cell culture<\/strong><\/p>\n

Morphology:<\/strong> mouse pluripotent stem cell colonies<\/p>\n

Cell culture method:<\/strong> monolayer<\/p>\n

Cell culture medium:<\/strong> DMEM (glucose 4.5 g\/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U\/ml<\/p>\n

Cell culture conditions:<\/strong> 37\u00b0C, 5% CO2<\/sub><\/p>\n

Passage protocol:<\/strong> passage with trypsin-EDTA 0.05%, split 1:3 — 1:6<\/p>\n

Cryoconservation:<\/strong> 90% KSR, 10% DMSO<\/p>\n

Cryoconservation cell concentration:<\/strong> 0.5 mln cells \/ ml<\/p>\n

Cell viability after cryoconservation:<\/strong> 70%<\/p>\n

Additional information:<\/strong> Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.<\/p>\n

\u00a0<\/strong><\/p>\n

References:<\/strong> https:\/\/doi.org\/10.1007\/s10616-014-9751-y<\/a><\/p>\n

<\/a>Cell line passport of MA07<\/h2>\n

 <\/p>\n

Catalogue number:<\/strong> MMES00010<\/p>\n

Name:<\/strong> MA07<\/p>\n

Description:<\/strong> mouse (Mus musculus<\/em>) embryonic stem cells derived from outbred BALB x 129\/Ola blastocyst<\/p>\n

Authors:<\/strong> Menzorov A.G.<\/p>\n

Contamination analysis:<\/strong> bacteria, fungi and mycoplasma not detected<\/p>\n

Karyotype:<\/strong> 2n=40, XX, chromosome number variability 39-43, tetraploid cells 24%, modal chromosome number 40<\/p>\n

Pluripotency:<\/strong> pluripotency is shown by expression of specific markers<\/p>\n

Additional characteristics:<\/strong><\/p>\n

Species control:<\/strong> cytogenetic<\/p>\n

Cryoconservation passage:<\/strong> 4<\/p>\n

Area of application:<\/strong> transgenesis, developmental biology<\/p>\n

 <\/p>\n

Source<\/strong><\/p>\n

Species:<\/strong> Mus musculus<\/em><\/p>\n

Tissue:<\/strong> blastocyst<\/p>\n

Date:<\/strong> 01.01.2016<\/p>\n

 <\/p>\n

Cell culture<\/strong><\/p>\n

Morphology:<\/strong> mouse pluripotent stem cell colonies<\/p>\n

Cell culture method:<\/strong> monolayer<\/p>\n

Cell culture medium:<\/strong> DMEM (glucose 4.5 g\/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U\/ml<\/p>\n

Cell culture conditions:<\/strong> 37\u00b0C, 5% CO2<\/sub><\/p>\n

Passage protocol:<\/strong> passage with trypsin-EDTA 0.05%, split 1:3 — 1:6<\/p>\n

Cryoconservation:<\/strong> 90% KSR, 10% DMSO<\/p>\n

Cryoconservation cell concentration:<\/strong> 0.5 mln cells \/ ml<\/p>\n

Cell viability after cryoconservation:<\/strong> 70%<\/p>\n

Additional information:<\/strong> Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.<\/p>\n

\u00a0<\/strong><\/p>\n

References:<\/strong> https:\/\/doi.org\/10.1007\/s10616-014-9751-y<\/a><\/p>\n

<\/a>Cell line passport of MA08<\/h2>\n

 <\/p>\n

Catalogue number:<\/strong> MMES00011<\/p>\n

Name:<\/strong> MA08<\/p>\n

Description:<\/strong> mouse (Mus musculus<\/em>) embryonic stem cells derived from outbred BALB x 129\/Ola blastocyst<\/p>\n

Authors:<\/strong> Menzorov A.G.<\/p>\n

Contamination analysis:<\/strong> bacteria, fungi and mycoplasma not detected<\/p>\n

Karyotype:<\/strong> 2n=40, XX, chromosome number variability 39-41, tetraploid cells 11%, modal chromosome number 40<\/p>\n

Pluripotency:<\/strong> pluripotency is shown by expression of specific markers<\/p>\n

Additional characteristics:<\/strong><\/p>\n

Species control:<\/strong> cytogenetic<\/p>\n

Cryoconservation passage:<\/strong> 4<\/p>\n

Area of application:<\/strong> transgenesis, developmental biology<\/p>\n

 <\/p>\n

Source<\/strong><\/p>\n

Species:<\/strong> Mus musculus<\/em><\/p>\n

Tissue:<\/strong> blastocyst<\/p>\n

Date:<\/strong> 01.01.2016<\/p>\n

 <\/p>\n

Cell culture<\/strong><\/p>\n

Morphology:<\/strong> mouse pluripotent stem cell colonies<\/p>\n

Cell culture method:<\/strong> monolayer<\/p>\n

Cell culture medium:<\/strong> DMEM (glucose 4.5 g\/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U\/ml<\/p>\n

Cell culture conditions:<\/strong> 37\u00b0C, 5% CO2<\/sub><\/p>\n

Passage protocol:<\/strong> passage with trypsin-EDTA 0.05%, split 1:3 — 1:6<\/p>\n

Cryoconservation:<\/strong> 90% KSR, 10% DMSO<\/p>\n

Cryoconservation cell concentration:<\/strong> 0.5 mln cells \/ ml<\/p>\n

Cell viability after cryoconservation:<\/strong> 70%<\/p>\n

Additional information:<\/strong> Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.<\/p>\n

\u00a0<\/strong><\/p>\n

References:<\/strong> https:\/\/doi.org\/10.1007\/s10616-014-9751-y<\/a><\/p>\n

<\/a>Cell line passport of MA09<\/h2>\n

 <\/p>\n

Catalogue number:<\/strong> MMES00012<\/p>\n

Name:<\/strong> MA09<\/p>\n

Description:<\/strong> mouse (Mus musculus<\/em>) embryonic stem cells derived from outbred BALB x 129\/Ola blastocyst<\/p>\n

Authors:<\/strong> Menzorov A.G.<\/p>\n

Contamination analysis:<\/strong> bacteria, fungi and mycoplasma not detected<\/p>\n

Karyotype:<\/strong> 2n=40, XY, chromosome number variability 39-41, tetraploid cells 8%, modal chromosome number 40<\/p>\n

Pluripotency:<\/strong> pluripotency is shown by expression of specific markers<\/p>\n

Additional characteristics:<\/strong><\/p>\n

Species control:<\/strong> cytogenetic<\/p>\n

Cryoconservation passage:<\/strong> 4<\/p>\n

Area of application:<\/strong> transgenesis, developmental biology<\/p>\n

 <\/p>\n

Source<\/strong><\/p>\n

Species:<\/strong> Mus musculus<\/em><\/p>\n

Tissue:<\/strong> blastocyst<\/p>\n

Date:<\/strong> 01.01.2016<\/p>\n

 <\/p>\n

Cell culture<\/strong><\/p>\n

Morphology:<\/strong> mouse pluripotent stem cell colonies<\/p>\n

Cell culture method:<\/strong> monolayer<\/p>\n

Cell culture medium:<\/strong> DMEM (glucose 4.5 g\/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U\/ml<\/p>\n

Cell culture conditions:<\/strong> 37\u00b0C, 5% CO2<\/sub><\/p>\n

Passage protocol:<\/strong> passage with trypsin-EDTA 0.05%, split 1:3 — 1:6<\/p>\n

Cryoconservation:<\/strong> 90% KSR, 10% DMSO<\/p>\n

Cryoconservation cell concentration:<\/strong> 0.5 mln cells \/ ml<\/p>\n

Cell viability after cryoconservation:<\/strong> 70%<\/p>\n

Additional information:<\/strong> Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.<\/p>\n

\u00a0<\/strong><\/p>\n

References:<\/strong> https:\/\/doi.org\/10.1007\/s10616-014-9751-y<\/a><\/p>\n

<\/a>Cell line passport of MA10<\/h2>\n

 <\/p>\n

Catalogue number:<\/strong> MMES00013<\/p>\n

Name:<\/strong> MA10<\/p>\n

Description:<\/strong> mouse (Mus musculus<\/em>) embryonic stem cells derived from outbred BALB x 129\/Ola blastocyst<\/p>\n

Authors:<\/strong> Menzorov A.G.<\/p>\n

Contamination analysis:<\/strong> bacteria, fungi and mycoplasma not detected<\/p>\n

Karyotype:<\/strong> 2n=40, XX, chromosome number variability 39-43, tetraploid cells 7%, modal chromosome number 40<\/p>\n

Pluripotency:<\/strong> pluripotency is shown by expression of specific markers<\/p>\n

Additional characteristics:<\/strong><\/p>\n

Species control:<\/strong> cytogenetic<\/p>\n

Cryoconservation passage:<\/strong> 4<\/p>\n

Area of application:<\/strong> transgenesis, developmental biology<\/p>\n

 <\/p>\n

Source<\/strong><\/p>\n

Species:<\/strong> Mus musculus<\/em><\/p>\n

Tissue:<\/strong> blastocyst<\/p>\n

Date:<\/strong> 01.01.2016<\/p>\n

 <\/p>\n

Cell culture<\/strong><\/p>\n

Morphology:<\/strong> mouse pluripotent stem cell colonies<\/p>\n

Cell culture method:<\/strong> monolayer<\/p>\n

Cell culture medium:<\/strong> DMEM (glucose 4.5 g\/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U\/ml<\/p>\n

Cell culture conditions:<\/strong> 37\u00b0C, 5% CO2<\/sub><\/p>\n

Passage protocol:<\/strong> passage with trypsin-EDTA 0.05%, split 1:3 — 1:6<\/p>\n

Cryoconservation:<\/strong> 90% KSR, 10% DMSO<\/p>\n

Cryoconservation cell concentration:<\/strong> 0.5 mln cells \/ ml<\/p>\n

Cell viability after cryoconservation:<\/strong> 70%<\/p>\n

Additional information:<\/strong> Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.<\/p>\n

\u00a0<\/strong><\/p>\n

References:<\/strong> https:\/\/doi.org\/10.1007\/s10616-014-9751-y<\/a><\/p>\n

<\/a>Cell line passport of MA11<\/h2>\n

 <\/p>\n

Catalogue number:<\/strong> MMES00014<\/p>\n

Name:<\/strong> MA11<\/p>\n

Description:<\/strong> mouse (Mus musculus<\/em>) embryonic stem cells derived from outbred BALB x 129\/Ola blastocyst<\/p>\n

Authors:<\/strong> Menzorov A.G.<\/p>\n

Contamination analysis:<\/strong> bacteria, fungi and mycoplasma not detected<\/p>\n

Karyotype:<\/strong> 2n=40, XX, chromosome number variability 39-41, tetraploid cells 20%, modal chromosome number 40<\/p>\n

Pluripotency:<\/strong> pluripotency is shown by expression of specific markers<\/p>\n

Additional characteristics:<\/strong><\/p>\n

Species control:<\/strong> cytogenetic<\/p>\n

Cryoconservation passage:<\/strong> 7<\/p>\n

Area of application:<\/strong> transgenesis, developmental biology<\/p>\n

 <\/p>\n

Source<\/strong><\/p>\n

Species:<\/strong> Mus musculus<\/em><\/p>\n

Tissue:<\/strong> blastocyst<\/p>\n

Date:<\/strong> 01.01.2016<\/p>\n

 <\/p>\n

Cell culture<\/strong><\/p>\n

Morphology:<\/strong> mouse pluripotent stem cell colonies<\/p>\n

Cell culture method:<\/strong> monolayer<\/p>\n

Cell culture medium:<\/strong> DMEM (glucose 4.5 g\/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U\/ml<\/p>\n

Cell culture conditions:<\/strong> 37\u00b0C, 5% CO2<\/sub><\/p>\n

Passage protocol:<\/strong> passage with trypsin-EDTA 0.05%, split 1:3 — 1:6<\/p>\n

Cryoconservation:<\/strong> 90% KSR, 10% DMSO<\/p>\n

Cryoconservation cell concentration:<\/strong> 0.5 mln cells \/ ml<\/p>\n

Cell viability after cryoconservation:<\/strong> 70%<\/p>\n

Additional information:<\/strong> Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.<\/p>\n

\u00a0<\/strong><\/p>\n

References:<\/strong> https:\/\/doi.org\/10.1007\/s10616-014-9751-y<\/a><\/p>\n

<\/a>Cell line passport of MA12<\/h2>\n

 <\/p>\n

Catalogue number:<\/strong> MMES00015<\/p>\n

Name:<\/strong> MA12<\/p>\n

Description:<\/strong> mouse (Mus musculus<\/em>) embryonic stem cells derived from outbred BALB x 129\/Ola blastocyst<\/p>\n

Authors:<\/strong> Menzorov A.G.<\/p>\n

Contamination analysis:<\/strong> bacteria, fungi and mycoplasma not detected<\/p>\n

Karyotype:<\/strong> 2n=40, XY, chromosome number variability 39-42, tetraploid cells 2%, modal chromosome number 40, 41<\/p>\n

Pluripotency:<\/strong> pluripotency is shown by expression of specific markers<\/p>\n

Additional characteristics:<\/strong><\/p>\n

Species control:<\/strong> cytogenetic<\/p>\n

Cryoconservation passage:<\/strong> 5<\/p>\n

Area of application:<\/strong> transgenesis, developmental biology<\/p>\n

 <\/p>\n

Source<\/strong><\/p>\n

Species:<\/strong> Mus musculus<\/em><\/p>\n

Tissue:<\/strong> blastocyst<\/p>\n

Date:<\/strong> 01.01.2016<\/p>\n

 <\/p>\n

Cell culture<\/strong><\/p>\n

Morphology:<\/strong> mouse pluripotent stem cell colonies<\/p>\n

Cell culture method:<\/strong> monolayer<\/p>\n

Cell culture medium:<\/strong> DMEM (glucose 4.5 g\/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U\/ml<\/p>\n

Cell culture conditions:<\/strong> 37\u00b0C, 5% CO2<\/sub><\/p>\n

Passage protocol:<\/strong> passage with trypsin-EDTA 0.05%, split 1:3 — 1:6<\/p>\n

Cryoconservation:<\/strong> 90% KSR, 10% DMSO<\/p>\n

Cryoconservation cell concentration:<\/strong> 0.5 mln cells \/ ml<\/p>\n

Cell viability after cryoconservation:<\/strong> 70%<\/p>\n

Additional information:<\/strong> Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.<\/p>\n

\u00a0<\/strong><\/p>\n

References:<\/strong> https:\/\/doi.org\/10.1007\/s10616-014-9751-y<\/a><\/p>\n

<\/a>Cell line passport of MA13<\/h2>\n

 <\/p>\n

Catalogue number:<\/strong> MMES00016<\/p>\n

Name:<\/strong> MA13<\/p>\n

Description:<\/strong> mouse (Mus musculus<\/em>) embryonic stem cells derived from outbred BALB x 129\/Ola blastocyst<\/p>\n

Authors:<\/strong> Menzorov A.G.<\/p>\n

Contamination analysis:<\/strong> bacteria, fungi and mycoplasma not detected<\/p>\n

Karyotype:<\/strong> 2n=40, X0, chromosome number variability 39-43, tetraploid cells 8,6%, modal chromosome number 39<\/p>\n

Pluripotency:<\/strong> pluripotency is shown by expression of specific markers<\/p>\n

Additional characteristics:<\/strong><\/p>\n

Species control:<\/strong> cytogenetic<\/p>\n

Cryoconservation passage:<\/strong> 4<\/p>\n

Area of application:<\/strong> transgenesis, developmental biology<\/p>\n

 <\/p>\n

Source<\/strong><\/p>\n

Species:<\/strong> Mus musculus<\/em><\/p>\n

Tissue:<\/strong> blastocyst<\/p>\n

Date:<\/strong> 01.01.2016<\/p>\n

 <\/p>\n

Cell culture<\/strong><\/p>\n

Morphology:<\/strong> mouse pluripotent stem cell colonies<\/p>\n

Cell culture method:<\/strong> monolayer<\/p>\n

Cell culture medium:<\/strong> DMEM (glucose 4.5 g\/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U\/ml<\/p>\n

Cell culture conditions:<\/strong> 37\u00b0C, 5% CO2<\/sub><\/p>\n

Passage protocol:<\/strong> passage with trypsin-EDTA 0.05%, split 1:3 — 1:6<\/p>\n

Cryoconservation:<\/strong> 90% KSR, 10% DMSO<\/p>\n

Cryoconservation cell concentration:<\/strong> 0.5 mln cells \/ ml<\/p>\n

Cell viability after cryoconservation:<\/strong> 70%<\/p>\n

Additional information:<\/strong> Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.<\/p>\n

\u00a0<\/strong><\/p>\n

References:<\/strong> https:\/\/doi.org\/10.1007\/s10616-014-9751-y<\/a><\/p>\n

<\/a>Cell line passport of MA15<\/h2>\n

 <\/p>\n

Catalogue number:<\/strong> MMES00017<\/p>\n

Name:<\/strong> MA15<\/p>\n

Description:<\/strong> mouse (Mus musculus<\/em>) embryonic stem cells derived from outbred BALB x 129\/Ola blastocyst<\/p>\n

Authors:<\/strong> Menzorov A.G.<\/p>\n

Contamination analysis:<\/strong> bacteria, fungi and mycoplasma not detected<\/p>\n

Karyotype:<\/strong> 2n=40, XY, chromosome number variability 39-45, tetraploid cells 5%, modal chromosome number 40<\/p>\n

Pluripotency:<\/strong> pluripotency is shown by teratoma formation in NU\/NU mice<\/p>\n

Additional characteristics:<\/strong><\/p>\n

Species control:<\/strong> cytogenetic<\/p>\n

Cryoconservation passage:<\/strong> 4<\/p>\n

Area of application:<\/strong> transgenesis, developmental biology<\/p>\n

 <\/p>\n

Source<\/strong><\/p>\n

Species:<\/strong> Mus musculus<\/em><\/p>\n

Tissue:<\/strong> blastocyst<\/p>\n

Date:<\/strong> 01.01.2016<\/p>\n

 <\/p>\n

Cell culture<\/strong><\/p>\n

Morphology:<\/strong> mouse pluripotent stem cell colonies<\/p>\n

Cell culture method:<\/strong> monolayer<\/p>\n

Cell culture medium:<\/strong> DMEM (glucose 4.5 g\/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U\/ml<\/p>\n

Cell culture conditions:<\/strong> 37\u00b0C, 5% CO2<\/sub><\/p>\n

Passage protocol:<\/strong> passage with trypsin-EDTA 0.05%, split 1:3 — 1:6<\/p>\n

Cryoconservation:<\/strong> 90% KSR, 10% DMSO<\/p>\n

Cryoconservation cell concentration:<\/strong> 0.5 mln cells \/ ml<\/p>\n

Cell viability after cryoconservation:<\/strong> 70%<\/p>\n

Additional information:<\/strong> Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.<\/p>\n

\u00a0<\/strong><\/p>\n

References:<\/strong> https:\/\/doi.org\/10.1007\/s10616-014-9751-y<\/a><\/p>\n

<\/a>Cell line passport of MC01<\/h2>\n

 <\/p>\n

Catalogue number:<\/strong> MMES00048<\/p>\n

Name:<\/strong> MC01<\/p>\n

Description:<\/strong> mouse (Mus musculus<\/em>) embryonic stem cells derived from C57BL x (outbred BALB x 129\/Ola) blastocyst<\/p>\n

Authors:<\/strong> Menzorov A.G.<\/p>\n

Contamination analysis:<\/strong> bacteria, fungi and mycoplasma not detected<\/p>\n

Karyotype:<\/strong> 2n=40, XX, chromosome number variability 39-45, tetraploid cells 32%, modal chromosome number 41, 40<\/p>\n

Pluripotency:<\/strong> pluripotency is shown by expression of specific markers<\/p>\n

Additional characteristics:<\/strong><\/p>\n

Species control:<\/strong> cytogenetic<\/p>\n

Cryoconservation passage:<\/strong> 4<\/p>\n

Area of application:<\/strong> transgenesis, developmental biology<\/p>\n

 <\/p>\n

Source<\/strong><\/p>\n

Species:<\/strong> Mus musculus<\/em><\/p>\n

Tissue:<\/strong> blastocyst<\/p>\n

Date:<\/strong> 01.01.2016<\/p>\n

 <\/p>\n

Cell culture<\/strong><\/p>\n

Morphology:<\/strong> mouse pluripotent stem cell colonies<\/p>\n

Cell culture method:<\/strong> monolayer<\/p>\n

Cell culture medium:<\/strong> DMEM (glucose 4.5 g\/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U\/ml<\/p>\n

Cell culture conditions:<\/strong> 37\u00b0C, 5% CO2<\/sub><\/p>\n

Passage protocol:<\/strong> passage with trypsin-EDTA 0.05%, split 1:3 — 1:6<\/p>\n

Cryoconservation:<\/strong> 90% KSR, 10% DMSO<\/p>\n

Cryoconservation cell concentration:<\/strong> 0.5 mln cells \/ ml<\/p>\n

Cell viability after cryoconservation:<\/strong> 70%<\/p>\n

Additional information:<\/strong> Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.<\/p>\n

\u00a0<\/strong><\/p>\n

References:<\/strong> https:\/\/doi.org\/10.1007\/s10616-014-9751-y<\/a><\/p>\n

<\/a>Cell line passport of MC02<\/h2>\n

 <\/p>\n

Catalogue number:<\/strong> MMES00049<\/p>\n

Name:<\/strong> MC02<\/p>\n

Description:<\/strong> mouse (Mus musculus<\/em>) embryonic stem cells derived from C57BL x (outbred BALB x 129\/Ola) blastocyst<\/p>\n

Authors:<\/strong> Menzorov A.G.<\/p>\n

Contamination analysis:<\/strong> bacteria, fungi and mycoplasma not detected<\/p>\n

Karyotype:<\/strong> 2n=40, XY, chromosome number variability 39-43, tetraploid cells 16%, modal chromosome number 41, 40<\/p>\n

Pluripotency:<\/strong> pluripotency is shown by expression of specific markers<\/p>\n

Additional characteristics:<\/strong><\/p>\n

Species control:<\/strong> cytogenetic<\/p>\n

Cryoconservation passage:<\/strong> 5<\/p>\n

Area of application:<\/strong> transgenesis, developmental biology<\/p>\n

 <\/p>\n

Source<\/strong><\/p>\n

Species:<\/strong> Mus musculus<\/em><\/p>\n

Tissue:<\/strong> blastocyst<\/p>\n

Date:<\/strong> 01.01.2016<\/p>\n

 <\/p>\n

Cell culture<\/strong><\/p>\n

Morphology:<\/strong> mouse pluripotent stem cell colonies<\/p>\n

Cell culture method:<\/strong> monolayer<\/p>\n

Cell culture medium:<\/strong> DMEM (glucose 4.5 g\/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U\/ml<\/p>\n

Cell culture conditions:<\/strong> 37\u00b0C, 5% CO2<\/sub><\/p>\n

Passage protocol:<\/strong> passage with trypsin-EDTA 0.05%, split 1:3 — 1:6<\/p>\n

Cryoconservation:<\/strong> 90% KSR, 10% DMSO<\/p>\n

Cryoconservation cell concentration:<\/strong> 0.5 mln cells \/ ml<\/p>\n

Cell viability after cryoconservation:<\/strong> 70%<\/p>\n

Additional information:<\/strong> Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.<\/p>\n

\u00a0<\/strong><\/p>\n

References:<\/strong> https:\/\/doi.org\/10.1007\/s10616-014-9751-y<\/a><\/p>\n

<\/a>Cell line passport of MC03<\/h2>\n

 <\/p>\n

Catalogue number:<\/strong> MMES00050<\/p>\n

Name:<\/strong> MC03<\/p>\n

Description:<\/strong> mouse (Mus musculus<\/em>) embryonic stem cells derived from C57BL x (outbred BALB x 129\/Ola) blastocyst<\/p>\n

Authors:<\/strong> Menzorov A.G.<\/p>\n

Contamination analysis:<\/strong> bacteria, fungi and mycoplasma not detected<\/p>\n

Karyotype:<\/strong> 2n=40, XY, chromosome number variability 39-45, tetraploid cells 6%, modal chromosome number 40<\/p>\n

Pluripotency:<\/strong> pluripotency is shown by teratoma formation in NU\/NU mice<\/p>\n

Additional characteristics:<\/strong><\/p>\n

Species control:<\/strong> cytogenetic<\/p>\n

Cryoconservation passage:<\/strong> 6<\/p>\n

Area of application:<\/strong> transgenesis, developmental biology<\/p>\n

 <\/p>\n

Source<\/strong><\/p>\n

Species:<\/strong> Mus musculus<\/em><\/p>\n

Tissue:<\/strong> blastocyst<\/p>\n

Date:<\/strong> 01.01.2016<\/p>\n

 <\/p>\n

Cell culture<\/strong><\/p>\n

Morphology:<\/strong> mouse pluripotent stem cell colonies<\/p>\n

Cell culture method:<\/strong> monolayer<\/p>\n

Cell culture medium:<\/strong> DMEM (glucose 4.5 g\/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U\/ml<\/p>\n

Cell culture conditions:<\/strong> 37\u00b0C, 5% CO2<\/sub><\/p>\n

Passage protocol:<\/strong> passage with trypsin-EDTA 0.25%, split 1:3 — 1:6<\/p>\n

Cryoconservation:<\/strong> 90% KSR, 10% DMSO<\/p>\n

Cryoconservation cell concentration:<\/strong> 0.5 mln cells \/ ml<\/p>\n

Cell viability after cryoconservation:<\/strong> 70%<\/p>\n

Additional information:<\/strong> Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.<\/p>\n

\u00a0<\/strong><\/p>\n

References:<\/strong> https:\/\/doi.org\/10.1007\/s10616-014-9751-y<\/a><\/p>\n

<\/a>Cell line passport of MC04<\/h2>\n

 <\/p>\n

Catalogue number:<\/strong> MMES00051<\/p>\n

Name:<\/strong> MC04<\/p>\n

Description:<\/strong> mouse (Mus musculus<\/em>) embryonic stem cells derived from C57BL x (outbred BALB x 129\/Ola) blastocyst<\/p>\n

Authors:<\/strong> Menzorov A.G.<\/p>\n

Contamination analysis:<\/strong> bacteria, fungi and mycoplasma not detected<\/p>\n

Karyotype:<\/strong> 2n=40, XY, chromosome number variability 41-45, tetraploid cells 15%, modal chromosome number 42<\/p>\n

Pluripotency:<\/strong> pluripotency is shown by expression of specific markers<\/p>\n

Additional characteristics:<\/strong><\/p>\n

Species control:<\/strong> cytogenetic<\/p>\n

Cryoconservation passage:<\/strong> 5<\/p>\n

Area of application:<\/strong> transgenesis, developmental biology<\/p>\n

 <\/p>\n

Source<\/strong><\/p>\n

Species:<\/strong> Mus musculus<\/em><\/p>\n

Tissue:<\/strong> blastocyst<\/p>\n

Date:<\/strong> 01.01.2016<\/p>\n

 <\/p>\n

Cell culture<\/strong><\/p>\n

Morphology:<\/strong> mouse pluripotent stem cell colonies<\/p>\n

Cell culture method:<\/strong> monolayer<\/p>\n

Cell culture medium:<\/strong> DMEM (glucose 4.5 g\/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U\/ml<\/p>\n

Cell culture conditions:<\/strong> 37\u00b0C, 5% CO2<\/sub><\/p>\n

Passage protocol:<\/strong> passage with trypsin-EDTA 0.05%, split 1:3 — 1:6<\/p>\n

Cryoconservation:<\/strong> 90% KSR, 10% DMSO<\/p>\n

Cryoconservation cell concentration:<\/strong> 0.5 mln cells \/ ml<\/p>\n

Cell viability after cryoconservation:<\/strong> 70%<\/p>\n

Additional information:<\/strong> Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.<\/p>\n

\u00a0<\/strong><\/p>\n

References:<\/strong> https:\/\/doi.org\/10.1007\/s10616-014-9751-y<\/a><\/p>\n

<\/a>Cell line passport of MC05<\/h2>\n

 <\/p>\n

Catalogue number:<\/strong> MMES00052<\/p>\n

Name:<\/strong> MC05<\/p>\n

Description:<\/strong> mouse (Mus musculus<\/em>) embryonic stem cells derived from C57BL x (outbred BALB x 129\/Ola) blastocyst<\/p>\n

Authors:<\/strong> Menzorov A.G.<\/p>\n

Contamination analysis:<\/strong> bacteria, fungi and mycoplasma not detected<\/p>\n

Karyotype:<\/strong> 2n=40, XY, chromosome number variability 39-40, tetraploid cells 15%, modal chromosome number 40<\/p>\n

Pluripotency:<\/strong> pluripotency is shown by expression of specific markers<\/p>\n

Additional characteristics:<\/strong><\/p>\n

Species control:<\/strong> cytogenetic<\/p>\n

Cryoconservation passage:<\/strong> 4<\/p>\n

Area of application:<\/strong> transgenesis, developmental biology<\/p>\n

 <\/p>\n

Source<\/strong><\/p>\n

Species:<\/strong> Mus musculus<\/em><\/p>\n

Tissue:<\/strong> blastocyst<\/p>\n

Date:<\/strong> 01.01.2016<\/p>\n

 <\/p>\n

Cell culture<\/strong><\/p>\n

Morphology:<\/strong> mouse pluripotent stem cell colonies<\/p>\n

Cell culture method:<\/strong> monolayer<\/p>\n

Cell culture medium:<\/strong> DMEM (glucose 4.5 g\/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U\/ml<\/p>\n

Cell culture conditions:<\/strong> 37\u00b0C, 5% CO2<\/sub><\/p>\n

Passage protocol:<\/strong> passage with trypsin-EDTA 0.05%, split 1:3 — 1:6<\/p>\n

Cryoconservation:<\/strong> 90% KSR, 10% DMSO<\/p>\n

Cryoconservation cell concentration:<\/strong> 0.5 mln cells \/ ml<\/p>\n

Cell viability after cryoconservation:<\/strong> 70%<\/p>\n

Additional information:<\/strong> Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.<\/p>\n

\u00a0<\/strong><\/p>\n

References:<\/strong> https:\/\/doi.org\/10.1007\/s10616-014-9751-y<\/a><\/p>\n

<\/a>Cell line passport of MC06<\/h2>\n

 <\/p>\n

Catalogue number:<\/strong> MMES00053<\/p>\n

Name:<\/strong> MC06<\/p>\n

Description:<\/strong> mouse (Mus musculus<\/em>) embryonic stem cells derived from C57BL x (outbred BALB x 129\/Ola) blastocyst<\/p>\n

Authors:<\/strong> Menzorov A.G.<\/p>\n

Contamination analysis:<\/strong> bacteria, fungi and mycoplasma not detected<\/p>\n

Karyotype:<\/strong> 2n=40, XX, chromosome number variability 39-41, tetraploid cells 52%, modal chromosome number 40, 78, 79<\/p>\n

Pluripotency:<\/strong> pluripotency is shown by expression of specific markers<\/p>\n

Additional characteristics:<\/strong><\/p>\n

Species control:<\/strong> cytogenetic<\/p>\n

Cryoconservation passage:<\/strong> 4<\/p>\n

Area of application:<\/strong> transgenesis, developmental biology<\/p>\n

 <\/p>\n

Source<\/strong><\/p>\n

Species:<\/strong> Mus musculus<\/em><\/p>\n

Tissue:<\/strong> blastocyst<\/p>\n

Date:<\/strong> 01.01.2016<\/p>\n

 <\/p>\n

Cell culture<\/strong><\/p>\n

Morphology:<\/strong> mouse pluripotent stem cell colonies<\/p>\n

Cell culture method:<\/strong> monolayer<\/p>\n

Cell culture medium:<\/strong> DMEM (glucose 4.5 g\/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U\/ml<\/p>\n

Cell culture conditions:<\/strong> 37\u00b0C, 5% CO2<\/sub><\/p>\n

Passage protocol:<\/strong> passage with trypsin-EDTA 0.05%, split 1:3 — 1:6<\/p>\n

Cryoconservation:<\/strong> 90% KSR, 10% DMSO<\/p>\n

Cryoconservation cell concentration:<\/strong> 0.5 mln cells \/ ml<\/p>\n

Cell viability after cryoconservation:<\/strong> 70%<\/p>\n

Additional information:<\/strong> Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.<\/p>\n

\u00a0<\/strong><\/p>\n

References:<\/strong> https:\/\/doi.org\/10.1007\/s10616-014-9751-y<\/a><\/p>\n

<\/a>Cell line passport of MC07<\/h2>\n

 <\/p>\n

Catalogue number:<\/strong> MMES00054<\/p>\n

Name:<\/strong> MC07<\/p>\n

Description:<\/strong> mouse (Mus musculus<\/em>) embryonic stem cells derived from C57BL x (outbred BALB x 129\/Ola) blastocyst<\/p>\n

Authors:<\/strong> Menzorov A.G.<\/p>\n

Contamination analysis:<\/strong> bacteria, fungi and mycoplasma not detected<\/p>\n

Karyotype:<\/strong> 2n=40, XX, chromosome number variability 40-43, tetraploid cells 18%, modal chromosome number 40<\/p>\n

Pluripotency:<\/strong> pluripotency is shown by expression of specific markers<\/p>\n

Additional characteristics:<\/strong><\/p>\n

Species control:<\/strong> cytogenetic<\/p>\n

Cryoconservation passage:<\/strong> 4<\/p>\n

Area of application:<\/strong> transgenesis, developmental biology<\/p>\n

 <\/p>\n

Source<\/strong><\/p>\n

Species:<\/strong> Mus musculus<\/em><\/p>\n

Tissue:<\/strong> blastocyst<\/p>\n

Date:<\/strong> 01.01.2016<\/p>\n

 <\/p>\n

Cell culture<\/strong><\/p>\n

Morphology:<\/strong> mouse pluripotent stem cell colonies<\/p>\n

Cell culture method:<\/strong> monolayer<\/p>\n

Cell culture medium:<\/strong> DMEM (glucose 4.5 g\/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U\/ml<\/p>\n

Cell culture conditions:<\/strong> 37\u00b0C, 5% CO2<\/sub><\/p>\n

Passage protocol:<\/strong> passage with trypsin-EDTA 0.05%, split 1:3 — 1:6<\/p>\n

Cryoconservation:<\/strong> 90% KSR, 10% DMSO<\/p>\n

Cryoconservation cell concentration:<\/strong> 0.5 mln cells \/ ml<\/p>\n

Cell viability after cryoconservation:<\/strong> 70%<\/p>\n

Additional information:<\/strong> Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.<\/p>\n

\u00a0<\/strong><\/p>\n

References:<\/strong> https:\/\/doi.org\/10.1007\/s10616-014-9751-y<\/a><\/p>\n

<\/a>Cell line passport of MC08<\/h2>\n

 <\/p>\n

Catalogue number:<\/strong> MMES00055<\/p>\n

Name:<\/strong> MC08<\/p>\n

Description:<\/strong> mouse (Mus musculus<\/em>) embryonic stem cells derived from C57BL x (outbred BALB x 129\/Ola) blastocyst<\/p>\n

Authors:<\/strong> Menzorov A.G.<\/p>\n

Contamination analysis:<\/strong> bacteria, fungi and mycoplasma not detected<\/p>\n

Karyotype:<\/strong> 2n=40, XY, chromosome number variability 39-41, tetraploid cells 21%, modal chromosome number 40<\/p>\n

Pluripotency:<\/strong> pluripotency is shown by expression of specific markers<\/p>\n

Additional characteristics:<\/strong><\/p>\n

Species control:<\/strong> cytogenetic<\/p>\n

Cryoconservation passage:<\/strong> 10<\/p>\n

Area of application:<\/strong> transgenesis, developmental biology<\/p>\n

 <\/p>\n

Source<\/strong><\/p>\n

Species:<\/strong> Mus musculus<\/em><\/p>\n

Tissue:<\/strong> blastocyst<\/p>\n

Date:<\/strong> 01.01.2016<\/p>\n

 <\/p>\n

Cell culture<\/strong><\/p>\n

Morphology:<\/strong> mouse pluripotent stem cell colonies<\/p>\n

Cell culture method:<\/strong> monolayer<\/p>\n

Cell culture medium:<\/strong> DMEM (glucose 4.5 g\/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U\/ml<\/p>\n

Cell culture conditions:<\/strong> 37\u00b0C, 5% CO2<\/sub><\/p>\n

Passage protocol:<\/strong> passage with trypsin-EDTA 0.05%, split 1:3 — 1:6<\/p>\n

Cryoconservation:<\/strong> 90% KSR, 10% DMSO<\/p>\n

Cryoconservation cell concentration:<\/strong> 0.5 mln cells \/ ml<\/p>\n

Cell viability after cryoconservation:<\/strong> 70%<\/p>\n

Additional information:<\/strong> Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.<\/p>\n

\u00a0<\/strong><\/p>\n

References:<\/strong> https:\/\/doi.org\/10.1007\/s10616-014-9751-y<\/a><\/p>\n

<\/a>Cell line passport of MC09<\/h2>\n

 <\/p>\n

Catalogue number:<\/strong> MMES00056<\/p>\n

Name:<\/strong> MC09<\/p>\n

Description:<\/strong> mouse (Mus musculus<\/em>) embryonic stem cells derived from C57BL x (outbred BALB x 129\/Ola) blastocyst<\/p>\n

Authors:<\/strong> Menzorov A.G.<\/p>\n

Contamination analysis:<\/strong> bacteria, fungi and mycoplasma not detected<\/p>\n

Karyotype:<\/strong> 2n=40, XX, chromosome number variability 40-43, tetraploid cells 18%, modal chromosome number 40<\/p>\n

Pluripotency:<\/strong> pluripotency is shown by expression of specific markers<\/p>\n

Additional characteristics:<\/strong><\/p>\n

Species control:<\/strong> cytogenetic<\/p>\n

Cryoconservation passage:<\/strong> 4<\/p>\n

Area of application:<\/strong> transgenesis, developmental biology<\/p>\n

 <\/p>\n

Source<\/strong><\/p>\n

Species:<\/strong> Mus musculus<\/em><\/p>\n

Tissue:<\/strong> blastocyst<\/p>\n

Date:<\/strong> 01.01.2016<\/p>\n

 <\/p>\n

Cell culture<\/strong><\/p>\n

Morphology:<\/strong> mouse pluripotent stem cell colonies<\/p>\n

Cell culture method:<\/strong> monolayer<\/p>\n

Cell culture medium:<\/strong> DMEM (glucose 4.5 g\/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U\/ml<\/p>\n

Cell culture conditions:<\/strong> 37\u00b0C, 5% CO2<\/sub><\/p>\n

Passage protocol:<\/strong> passage with trypsin-EDTA 0.05%, split 1:3 — 1:6<\/p>\n

Cryoconservation:<\/strong> 90% KSR, 10% DMSO<\/p>\n

Cryoconservation cell concentration:<\/strong> 0.5 mln cells \/ ml<\/p>\n

Cell viability after cryoconservation:<\/strong> 70%<\/p>\n

Additional information:<\/strong> Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.<\/p>\n

\u00a0<\/strong><\/p>\n

References:<\/strong> https:\/\/doi.org\/10.1007\/s10616-014-9751-y<\/a><\/p>\n

<\/a>Cell line passport of MC10<\/h2>\n

 <\/p>\n

Catalogue number:<\/strong> MMES00057<\/p>\n

Name:<\/strong> MC10<\/p>\n

Description:<\/strong> mouse (Mus musculus<\/em>) embryonic stem cells derived from C57BL x (outbred BALB x 129\/Ola) blastocyst<\/p>\n

Authors:<\/strong> Menzorov A.G.<\/p>\n

Contamination analysis:<\/strong> bacteria, fungi and mycoplasma not detected<\/p>\n

Karyotype:<\/strong> 2n=40, XY, chromosome number variability 40-43, tetraploid cells 18%, modal chromosome number 40<\/p>\n

Pluripotency:<\/strong> pluripotency is shown by expression of specific markers<\/p>\n

Additional characteristics:<\/strong><\/p>\n

Species control:<\/strong> cytogenetic<\/p>\n

Cryoconservation passage:<\/strong> 11<\/p>\n

Area of application:<\/strong> transgenesis, developmental biology<\/p>\n

 <\/p>\n

Source<\/strong><\/p>\n

Species:<\/strong> Mus musculus<\/em><\/p>\n

Tissue:<\/strong> blastocyst<\/p>\n

Date:<\/strong> 01.01.2016<\/p>\n

 <\/p>\n

Cell culture<\/strong><\/p>\n

Morphology:<\/strong> mouse pluripotent stem cell colonies<\/p>\n

Cell culture method:<\/strong> monolayer<\/p>\n

Cell culture medium:<\/strong> DMEM (glucose 4.5 g\/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U\/ml<\/p>\n

Cell culture conditions:<\/strong> 37\u00b0C, 5% CO2<\/sub><\/p>\n

Passage protocol:<\/strong> passage with trypsin-EDTA 0.05%, split 1:3 — 1:6<\/p>\n

Cryoconservation:<\/strong> 90% KSR, 10% DMSO<\/p>\n

Cryoconservation cell concentration:<\/strong> 0.5 mln cells \/ ml<\/p>\n

Cell viability after cryoconservation:<\/strong> 70%<\/p>\n

Additional information:<\/strong> Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.<\/p>\n

\u00a0<\/strong><\/p>\n

References:<\/strong> https:\/\/doi.org\/10.1007\/s10616-014-9751-y<\/a><\/p>\n

<\/a>Cell line passport of MC11<\/h2>\n

 <\/p>\n

Catalogue number:<\/strong> MMES00058<\/p>\n

Name:<\/strong> MC11<\/p>\n

Description:<\/strong> mouse (Mus musculus<\/em>) embryonic stem cells derived from C57BL x (outbred BALB x 129\/Ola) blastocyst<\/p>\n

Authors:<\/strong> Menzorov A.G.<\/p>\n

Contamination analysis:<\/strong> bacteria, fungi and mycoplasma not detected<\/p>\n

Karyotype:<\/strong> 2n=40, XX, chromosome number variability 40-43, tetraploid cells 25%, modal chromosome number 40<\/p>\n

Pluripotency:<\/strong> pluripotency is shown by teratoma formation in NU\/NU mice<\/p>\n

Additional characteristics:<\/strong><\/p>\n

Species control:<\/strong> cytogenetic<\/p>\n

Cryoconservation passage:<\/strong> 6<\/p>\n

Area of application:<\/strong> transgenesis, developmental biology<\/p>\n

 <\/p>\n

Source<\/strong><\/p>\n

Species:<\/strong> Mus musculus<\/em><\/p>\n

Tissue:<\/strong> blastocyst<\/p>\n

Date:<\/strong> 01.01.2016<\/p>\n

 <\/p>\n

Cell culture<\/strong><\/p>\n

Morphology:<\/strong> mouse pluripotent stem cell colonies<\/p>\n

Cell culture method:<\/strong> monolayer<\/p>\n

Cell culture medium:<\/strong> DMEM (glucose 4.5 g\/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U\/ml<\/p>\n

Cell culture conditions:<\/strong> 37\u00b0C, 5% CO2<\/sub><\/p>\n

Passage protocol:<\/strong> passage with trypsin-EDTA 0.05%, split 1:3 — 1:6<\/p>\n

Cryoconservation:<\/strong> 90% KSR, 10% DMSO<\/p>\n

Cryoconservation cell concentration:<\/strong> 0.5 mln cells \/ ml<\/p>\n

Cell viability after cryoconservation:<\/strong> 70%<\/p>\n

Additional information:<\/strong> Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.<\/p>\n

\u00a0<\/strong><\/p>\n

References:<\/strong> https:\/\/doi.org\/10.1007\/s10616-014-9751-y<\/a><\/p>\n

<\/a>Cell line passport of MC12<\/h2>\n

 <\/p>\n

Catalogue number:<\/strong> MMES00059<\/p>\n

Name:<\/strong> MC12<\/p>\n

Description:<\/strong> mouse (Mus musculus<\/em>) embryonic stem cells derived from C57BL x (outbred BALB x 129\/Ola) blastocyst<\/p>\n

Authors:<\/strong> Menzorov A.G.<\/p>\n

Contamination analysis:<\/strong> bacteria, fungi and mycoplasma not detected<\/p>\n

Karyotype:<\/strong> 2n=40, XY, chromosome number variability 39-42, tetraploid cells 8%, modal chromosome number 40<\/p>\n

Pluripotency:<\/strong> pluripotency is shown by teratoma formation in NU\/NU mice<\/p>\n

Additional characteristics:<\/strong><\/p>\n

Species control:<\/strong> cytogenetic<\/p>\n

Cryoconservation passage:<\/strong> 3<\/p>\n

Area of application:<\/strong> transgenesis, developmental biology<\/p>\n

 <\/p>\n

Source<\/strong><\/p>\n

Species:<\/strong> Mus musculus<\/em><\/p>\n

Tissue:<\/strong> blastocyst<\/p>\n

Date:<\/strong> 01.01.2016<\/p>\n

 <\/p>\n

Cell culture<\/strong><\/p>\n

Morphology:<\/strong> mouse pluripotent stem cell colonies<\/p>\n

Cell culture method:<\/strong> monolayer<\/p>\n

Cell culture medium:<\/strong> DMEM (glucose 4.5 g\/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U\/ml<\/p>\n

Cell culture conditions:<\/strong> 37\u00b0C, 5% CO2<\/sub><\/p>\n

Passage protocol:<\/strong> passage with trypsin-EDTA 0.05%, split 1:3 — 1:6<\/p>\n

Cryoconservation:<\/strong> 90% KSR, 10% DMSO<\/p>\n

Cryoconservation cell concentration:<\/strong> 0.5 mln cells \/ ml<\/p>\n

Cell viability after cryoconservation:<\/strong> 70%<\/p>\n

Additional information:<\/strong> Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.<\/p>\n

\u00a0<\/strong><\/p>\n

References:<\/strong> https:\/\/doi.org\/10.1007\/s10616-014-9751-y<\/a><\/p>\n

<\/a>Cell line passport of MC13<\/h2>\n

 <\/p>\n

Catalogue number:<\/strong> MMES00060<\/p>\n

Name:<\/strong> MC13<\/p>\n

Description:<\/strong> mouse (Mus musculus<\/em>) embryonic stem cells derived from C57BL x (outbred BALB x 129\/Ola) blastocyst<\/p>\n

Authors:<\/strong> Menzorov A.G.<\/p>\n

Contamination analysis:<\/strong> bacteria, fungi and mycoplasma not detected<\/p>\n

Karyotype:<\/strong> 2n=40, XX, chromosome number variability 39-43, tetraploid cells 8%, modal chromosome number 40<\/p>\n

Pluripotency:<\/strong> pluripotency is shown by expression of specific markers<\/p>\n

Additional characteristics:<\/strong><\/p>\n

Species control:<\/strong> cytogenetic<\/p>\n

Cryoconservation passage:<\/strong> 8<\/p>\n

Area of application:<\/strong> transgenesis, developmental biology<\/p>\n

 <\/p>\n

Source<\/strong><\/p>\n

Species:<\/strong> Mus musculus<\/em><\/p>\n

Tissue:<\/strong> blastocyst<\/p>\n

Date:<\/strong> 01.01.2016<\/p>\n

 <\/p>\n

Cell culture<\/strong><\/p>\n

Morphology:<\/strong> mouse pluripotent stem cell colonies<\/p>\n

Cell culture method:<\/strong> monolayer<\/p>\n

Cell culture medium:<\/strong> DMEM (glucose 4.5 g\/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U\/ml<\/p>\n

Cell culture conditions:<\/strong> 37\u00b0C, 5% CO2<\/sub><\/p>\n

Passage protocol:<\/strong> passage with trypsin-EDTA 0.05%, split 1:3 — 1:6<\/p>\n

Cryoconservation:<\/strong> 90% KSR, 10% DMSO<\/p>\n

Cryoconservation cell concentration:<\/strong> 0.5 mln cells \/ ml<\/p>\n

Cell viability after cryoconservation:<\/strong> 70%<\/p>\n

Additional information:<\/strong> Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.<\/p>\n

\u00a0<\/strong><\/p>\n

References:<\/strong> https:\/\/doi.org\/10.1007\/s10616-014-9751-y<\/a><\/p>\n

<\/a>Cell line passport of MC15<\/h2>\n

 <\/p>\n

Catalogue number:<\/strong> MMES00061<\/p>\n

Name:<\/strong> MC15<\/p>\n

Description:<\/strong> mouse (Mus musculus<\/em>) embryonic stem cells derived from C57BL x (outbred BALB x 129\/Ola) blastocyst<\/p>\n

Authors:<\/strong> Menzorov A.G.<\/p>\n

Contamination analysis:<\/strong> bacteria, fungi and mycoplasma not detected<\/p>\n

Karyotype:<\/strong> 2n=40, XY, chromosome number variability 40-44, tetraploid cells 20%, modal chromosome number 41, 42, 43<\/p>\n

Pluripotency:<\/strong> pluripotency is shown by expression of specific markers<\/p>\n

Additional characteristics:<\/strong><\/p>\n

Species control:<\/strong> cytogenetic<\/p>\n

Cryoconservation passage:<\/strong> 3<\/p>\n

Area of application:<\/strong> transgenesis, developmental biology<\/p>\n

 <\/p>\n

Source<\/strong><\/p>\n

Species:<\/strong> Mus musculus<\/em><\/p>\n

Tissue:<\/strong> blastocyst<\/p>\n

Date:<\/strong> 01.01.2016<\/p>\n

 <\/p>\n

Cell culture<\/strong><\/p>\n

Morphology:<\/strong> mouse pluripotent stem cell colonies<\/p>\n

Cell culture method:<\/strong> monolayer<\/p>\n

Cell culture medium:<\/strong> DMEM (glucose 4.5 g\/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U\/ml<\/p>\n

Cell culture conditions:<\/strong> 37\u00b0C, 5% CO2<\/sub><\/p>\n

Passage protocol:<\/strong> passage with trypsin-EDTA 0.05%, split 1:3 — 1:6<\/p>\n

Cryoconservation:<\/strong> 90% KSR, 10% DMSO<\/p>\n

Cryoconservation cell concentration:<\/strong> 0.5 mln cells \/ ml<\/p>\n

Cell viability after cryoconservation:<\/strong> 70%<\/p>\n

Additional information:<\/strong> Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.<\/p>\n

\u00a0<\/strong><\/p>\n

References:<\/strong> https:\/\/doi.org\/10.1007\/s10616-014-9751-y<\/a><\/p>\n

<\/a>Cell line passport of MD01<\/h2>\n

 <\/p>\n

Catalogue number:<\/strong> MMES00062<\/p>\n

Name:<\/strong> MD01<\/p>\n

Description:<\/strong> mouse (Mus musculus<\/em>) embryonic stem cells derived from DD\/c x (outbred BALB x 129\/Ola) blastocyst<\/p>\n

Authors:<\/strong> Menzorov A.G.<\/p>\n

Contamination analysis:<\/strong> bacteria, fungi and mycoplasma not detected<\/p>\n

Karyotype:<\/strong> 2n=40, XX, chromosome number variability 39-44, tetraploid cells 26%, modal chromosome number 40<\/p>\n

Pluripotency:<\/strong> pluripotency is shown by expression of specific markers<\/p>\n

Additional characteristics:<\/strong><\/p>\n

Species control:<\/strong> cytogenetic<\/p>\n

Cryoconservation passage:<\/strong> 7<\/p>\n

Area of application:<\/strong> transgenesis, developmental biology<\/p>\n

 <\/p>\n

Source<\/strong><\/p>\n

Species:<\/strong> Mus musculus<\/em><\/p>\n

Tissue:<\/strong> blastocyst<\/p>\n

Date:<\/strong> 01.01.2016<\/p>\n

 <\/p>\n

Cell culture<\/strong><\/p>\n

Morphology:<\/strong> mouse pluripotent stem cell colonies<\/p>\n

Cell culture method:<\/strong> monolayer<\/p>\n

Cell culture medium:<\/strong> DMEM (glucose 4.5 g\/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U\/ml<\/p>\n

Cell culture conditions:<\/strong> 37\u00b0C, 5% CO2<\/sub><\/p>\n

Passage protocol:<\/strong> passage with trypsin-EDTA 0.05%, split 1:3 — 1:6<\/p>\n

Cryoconservation:<\/strong> 90% KSR, 10% DMSO<\/p>\n

Cryoconservation cell concentration:<\/strong> 0.5 mln cells \/ ml<\/p>\n

Cell viability after cryoconservation:<\/strong> 70%<\/p>\n

Additional information:<\/strong> Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.<\/p>\n

\u00a0<\/strong><\/p>\n

References:<\/strong> https:\/\/doi.org\/10.1007\/s10616-014-9751-y<\/a><\/p>\n

<\/a>Cell line passport of MD02<\/h2>\n

 <\/p>\n

Catalogue number:<\/strong> MMES00063<\/p>\n

Name:<\/strong> MD02<\/p>\n

Description:<\/strong> mouse (Mus musculus<\/em>) embryonic stem cells derived from DD\/c x (outbred BALB x 129\/Ola) blastocyst<\/p>\n

Authors:<\/strong> Menzorov A.G.<\/p>\n

Contamination analysis:<\/strong> bacteria, fungi and mycoplasma not detected<\/p>\n

Karyotype:<\/strong> 2n=40, XXX0, chromosome number variability 40-48, 54-60, tetraploid cells 95%, modal chromosome number 80<\/p>\n

Pluripotency:<\/strong> pluripotency is shown by expression of specific markers<\/p>\n

Additional characteristics:<\/strong><\/p>\n

Species control:<\/strong> cytogenetic<\/p>\n

Cryoconservation passage:<\/strong> 6<\/p>\n

Area of application:<\/strong> transgenesis, developmental biology<\/p>\n

 <\/p>\n

Source<\/strong><\/p>\n

Species:<\/strong> Mus musculus<\/em><\/p>\n

Tissue:<\/strong> blastocyst<\/p>\n

Date:<\/strong> 01.01.2016<\/p>\n

 <\/p>\n

Cell culture<\/strong><\/p>\n

Morphology:<\/strong> mouse pluripotent stem cell colonies<\/p>\n

Cell culture method:<\/strong> monolayer<\/p>\n

Cell culture medium:<\/strong> DMEM (glucose 4.5 g\/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U\/ml<\/p>\n

Cell culture conditions:<\/strong> 37\u00b0C, 5% CO2<\/sub><\/p>\n

Passage protocol:<\/strong> passage with trypsin-EDTA 0.05%, split 1:3 — 1:6<\/p>\n

Cryoconservation:<\/strong> 90% KSR, 10% DMSO<\/p>\n

Cryoconservation cell concentration:<\/strong> 0.5 mln cells \/ ml<\/p>\n

Cell viability after cryoconservation:<\/strong> 70%<\/p>\n

Additional information:<\/strong> Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.<\/p>\n

\u00a0<\/strong><\/p>\n

References:<\/strong> https:\/\/doi.org\/10.1007\/s10616-014-9751-y<\/a><\/p>\n

<\/h2>\n

<\/a>Hybrid cells<\/h1>\n

<\/a>Cell line passport of tme13<\/h2>\n

 <\/p>\n

Catalogue number:<\/strong> MMHC00042<\/p>\n

Name:<\/strong> tme13<\/p>\n

Description:<\/strong> hybrid cells produced by mouse tau-GFP ES cell and m5S embryonic fibroblast fusion, embryonic stem cell phenotype<\/p>\n

Authors:<\/strong> Matveeva N.M.<\/p>\n

Contamination analysis:<\/strong> bacteria, fungi and mycoplasma not detected<\/p>\n

Karyotype:<\/strong> 4n=80, XX0, 83% of the cells have number of chromosomes 66-79<\/p>\n

Pluripotency:<\/strong> pluripotency is shown by expression of specific markers<\/p>\n

Additional characteristics:<\/strong><\/p>\n

Species control:<\/strong> cytogenetic<\/p>\n

Cryoconservation passage:<\/strong> 10<\/p>\n

Area of application:<\/strong> study of pluripotency, developmental biology<\/p>\n

 <\/p>\n

Source<\/strong><\/p>\n

Species:<\/strong> Mus musculus<\/em><\/p>\n

Tissue:<\/strong><\/p>\n

Date:<\/strong> 01.01.2017<\/p>\n

 <\/p>\n

Cell culture<\/strong><\/p>\n

Morphology:<\/strong> mouse pluripotent stem cell colonies<\/p>\n

Cell culture method:<\/strong> monolayer<\/p>\n

Cell culture medium:<\/strong> DMEM (glucose 4.5 g\/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U\/ml<\/p>\n

Cell culture conditions:<\/strong> 37\u00b0C, 5% CO2<\/sub><\/p>\n

Passage protocol:<\/strong> passage with trypsin-EDTA 0.05%, split 1:3 — 1:6<\/p>\n

Cryoconservation:<\/strong> 90% KSR, 10% DMSO<\/p>\n

Cryoconservation cell concentration:<\/strong> 0.5 mln cells \/ ml<\/p>\n

Cell viability after cryoconservation:<\/strong> 70%<\/p>\n

Additional information:<\/strong> Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.<\/p>\n

\u00a0<\/strong><\/p>\n

References:<\/strong> https:\/\/doi.org\/10.1038\/s41598-017-18352-4<\/a><\/p>\n

<\/a>Cell line passport of tme14<\/h2>\n

 <\/p>\n

Catalogue number:<\/strong> MMHC00043<\/p>\n

Name:<\/strong> tme14<\/p>\n

Description:<\/strong> hybrid cells produced by mouse tau-GFP ES cell and m5S embryonic fibroblast fusion, embryonic stem cell phenotype<\/p>\n

Authors:<\/strong> Matveeva N.M.<\/p>\n

Contamination analysis:<\/strong> bacteria, fungi and mycoplasma not detected<\/p>\n

Karyotype:<\/strong> 4n=80, XX0, 92% of the cells have number of chromosomes 69-77<\/p>\n

Pluripotency:<\/strong> pluripotency is shown by expression of specific markers<\/p>\n

Additional characteristics:<\/strong><\/p>\n

Species control:<\/strong> cytogenetic<\/p>\n

Cryoconservation passage:<\/strong> 17<\/p>\n

Area of application:<\/strong> study of pluripotency, developmental biology<\/p>\n

 <\/p>\n

Source<\/strong><\/p>\n

Species:<\/strong> Mus musculus<\/em><\/p>\n

Tissue:<\/strong><\/p>\n

Date:<\/strong> 01.01.2017<\/p>\n

 <\/p>\n

Cell culture<\/strong><\/p>\n

Morphology:<\/strong> mouse pluripotent stem cell colonies<\/p>\n

Cell culture method:<\/strong> monolayer<\/p>\n

Cell culture medium:<\/strong> DMEM (glucose 4.5 g\/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U\/ml<\/p>\n

Cell culture conditions:<\/strong> 37\u00b0C, 5% CO2<\/sub><\/p>\n

Passage protocol:<\/strong> passage with trypsin-EDTA 0.05%, split 1:3 — 1:6<\/p>\n

Cryoconservation:<\/strong> 90% KSR, 10% DMSO<\/p>\n

Cryoconservation cell concentration:<\/strong> 0.5 mln cells \/ ml<\/p>\n

Cell viability after cryoconservation:<\/strong> 70%<\/p>\n

Additional information:<\/strong> Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.<\/p>\n

\u00a0<\/strong><\/p>\n

References:<\/strong> https:\/\/doi.org\/10.1038\/s41598-017-18352-4<\/a><\/p>\n

<\/a>Cell line passport of tme17<\/h2>\n

 <\/p>\n

Catalogue number:<\/strong> MMHC00044<\/p>\n

Name:<\/strong> tme17<\/p>\n

Description:<\/strong> hybrid cells produced by mouse tau-GFP ES cell and m5S embryonic fibroblast fusion, embryonic stem cell phenotype<\/p>\n

Authors:<\/strong> Matveeva N.M.<\/p>\n

Contamination analysis:<\/strong> bacteria, fungi and mycoplasma not detected<\/p>\n

Karyotype:<\/strong> 4n=80, XX0, 75% of the cells have number of chromosomes 71-79<\/p>\n

Pluripotency:<\/strong> pluripotency is shown by expression of specific markers<\/p>\n

Additional characteristics:<\/strong><\/p>\n

Species control:<\/strong> cytogenetic<\/p>\n

Cryoconservation passage:<\/strong> 10<\/p>\n

Area of application:<\/strong> study of pluripotency, developmental biology<\/p>\n

 <\/p>\n

Source<\/strong><\/p>\n

Species:<\/strong> Mus musculus<\/em><\/p>\n

Tissue:<\/strong><\/p>\n

Date:<\/strong> 01.01.2017<\/p>\n

 <\/p>\n

Cell culture<\/strong><\/p>\n

Morphology:<\/strong> mouse pluripotent stem cell colonies<\/p>\n

Cell culture method:<\/strong> monolayer<\/p>\n

Cell culture medium:<\/strong> DMEM (glucose 4.5 g\/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U\/ml<\/p>\n

Cell culture conditions:<\/strong> 37\u00b0C, 5% CO2<\/sub><\/p>\n

Passage protocol:<\/strong> passage with trypsin-EDTA 0.05%, split 1:3 — 1:6<\/p>\n

Cryoconservation:<\/strong> 90% KSR, 10% DMSO<\/p>\n

Cryoconservation cell concentration:<\/strong> 0.5 mln cells \/ ml<\/p>\n

Cell viability after cryoconservation:<\/strong> 70%<\/p>\n

Additional information:<\/strong> Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.<\/p>\n

\u00a0<\/strong><\/p>\n

References:<\/strong> https:\/\/doi.org\/10.1038\/s41598-017-18352-4<\/a><\/p>\n

<\/a>Cell line passport of tmf1<\/h2>\n

 <\/p>\n

Catalogue number:<\/strong> MMHC00045<\/p>\n

Name:<\/strong> tmf1<\/p>\n

Description:<\/strong> hybrid cells produced by mouse tau-GFP ES cell and m5S embryonic fibroblast fusion, fibroblast phenotype<\/p>\n

Authors:<\/strong> Matveeva N.M.<\/p>\n

Contamination analysis:<\/strong> bacteria, fungi and mycoplasma not detected<\/p>\n

Karyotype:<\/strong> 4n=80, XXY, 90% of the cells have number of chromosomes 69-82<\/p>\n

Pluripotency:<\/strong><\/p>\n

Additional characteristics:<\/strong><\/p>\n

Species control:<\/strong> cytogenetic<\/p>\n

Cryoconservation passage:<\/strong> 7<\/p>\n

Area of application:<\/strong> study of pluripotency, developmental biology<\/p>\n

 <\/p>\n

Source<\/strong><\/p>\n

Species:<\/strong> Mus musculus<\/em><\/p>\n

Tissue:<\/strong><\/p>\n

Date:<\/strong> 01.01.2017<\/p>\n

 <\/p>\n

Cell culture<\/strong><\/p>\n

Morphology:<\/strong> fibroblast morphology<\/p>\n

Cell culture method:<\/strong> monolayer<\/p>\n

Cell culture medium:<\/strong> DMEM (glucose 4.5 g\/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U\/ml<\/p>\n

Cell culture conditions:<\/strong> 37\u00b0C, 5% CO2<\/sub><\/p>\n

Passage protocol:<\/strong> passage with trypsin-EDTA 0.25%, split 1:3 — 1:6<\/p>\n

Cryoconservation:<\/strong> 90% FBS, 10% DMSO<\/p>\n

Cryoconservation cell concentration:<\/strong> 0.5 mln cells \/ ml<\/p>\n

Cell viability after cryoconservation:<\/strong> 70%<\/p>\n

Additional information:<\/strong><\/p>\n

\u00a0<\/strong><\/p>\n

References:<\/strong> https:\/\/doi.org\/10.1038\/s41598-017-18352-4<\/a><\/p>\n

<\/a>Cell line passport of tmf2<\/h2>\n

 <\/p>\n

Catalogue number:<\/strong> MMHC00046<\/p>\n

Name:<\/strong> tmf2<\/p>\n

Description:<\/strong> hybrid cells produced by mouse tau-GFP ES cell and m5S embryonic fibroblast fusion, fibroblast phenotype<\/p>\n

Authors:<\/strong> Matveeva N.M.<\/p>\n

Contamination analysis:<\/strong> bacteria, fungi and mycoplasma not detected<\/p>\n

Karyotype:<\/strong> 4n=80, XXY, 76% of the cells have number of chromosomes 71-82<\/p>\n

Pluripotency:<\/strong><\/p>\n

Additional characteristics:<\/strong><\/p>\n

Species control:<\/strong> cytogenetic<\/p>\n

Cryoconservation passage:<\/strong> 6<\/p>\n

Area of application:<\/strong> study of pluripotency, developmental biology<\/p>\n

 <\/p>\n

Source<\/strong><\/p>\n

Species:<\/strong> Mus musculus<\/em><\/p>\n

Tissue:<\/strong><\/p>\n

Date:<\/strong> 01.01.2017<\/p>\n

 <\/p>\n

Cell culture<\/strong><\/p>\n

Morphology:<\/strong> fibroblast morphology<\/p>\n

Cell culture method:<\/strong> monolayer<\/p>\n

Cell culture medium:<\/strong> DMEM (glucose 4.5 g\/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U\/ml<\/p>\n

Cell culture conditions:<\/strong> 37\u00b0C, 5% CO2<\/sub><\/p>\n

Passage protocol:<\/strong> passage with trypsin-EDTA 0.25%, split 1:3 — 1:6<\/p>\n

Cryoconservation:<\/strong> 90% FBS, 10% DMSO<\/p>\n

Cryoconservation cell concentration:<\/strong> 0.5 mln cells \/ ml<\/p>\n

Cell viability after cryoconservation:<\/strong> 70%<\/p>\n

Additional information:<\/strong><\/p>\n

\u00a0<\/strong><\/p>\n

References:<\/strong> https:\/\/doi.org\/10.1038\/s41598-017-18352-4<\/a><\/p>\n

<\/a>Cell line passport of tmf5<\/h2>\n

 <\/p>\n

Catalogue number:<\/strong> MMHC00047<\/p>\n

Name:<\/strong> tmf5<\/p>\n

Description:<\/strong> hybrid cells produced by mouse tau-GFP ES cell and m5S embryonic fibroblast fusion, fibroblast phenotype<\/p>\n

Authors:<\/strong> Matveeva N.M.<\/p>\n

Contamination analysis:<\/strong> bacteria, fungi and mycoplasma not detected<\/p>\n

Karyotype:<\/strong> 4n=80, XXY, 86% of the cells have number of chromosomes 73-81<\/p>\n

Pluripotency:<\/strong><\/p>\n

Additional characteristics:<\/strong><\/p>\n

Species control:<\/strong> cytogenetic<\/p>\n

Cryoconservation passage:<\/strong> 6<\/p>\n

Area of application:<\/strong> study of pluripotency, developmental biology<\/p>\n

 <\/p>\n

Source<\/strong><\/p>\n

Species:<\/strong> Mus musculus<\/em><\/p>\n

Tissue:<\/strong><\/p>\n

Date:<\/strong> 01.01.2017<\/p>\n

 <\/p>\n

Cell culture<\/strong><\/p>\n

Morphology:<\/strong> fibroblast morphology<\/p>\n

Cell culture method:<\/strong> monolayer<\/p>\n

Cell culture medium:<\/strong> DMEM (glucose 4.5 g\/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U\/ml<\/p>\n

Cell culture conditions:<\/strong> 37\u00b0C, 5% CO2<\/sub><\/p>\n

Passage protocol:<\/strong> passage with trypsin-EDTA 0.25%, split 1:3 — 1:6<\/p>\n

Cryoconservation:<\/strong> 90% FBS, 10% DMSO<\/p>\n

Cryoconservation cell concentration:<\/strong> 0.5 mln cells \/ ml<\/p>\n

Cell viability after cryoconservation:<\/strong> 70%<\/p>\n

Additional information:<\/strong><\/p>\n

\u00a0<\/strong><\/p>\n

References:<\/strong> https:\/\/doi.org\/10.1038\/s41598-017-18352-4<\/a><\/p>\n

<\/h2>\n

<\/a>American mink pluripotent stem cells<\/h1>\n

<\/a>Cell line passport of MES12<\/h2>\n

 <\/p>\n

Catalogue number:<\/strong> NVES00003<\/p>\n

Name:<\/strong> MES12<\/p>\n

Description:<\/strong> american mink (Neovison vison) embryonic stem cells derived from morula<\/p>\n

Authors:<\/strong> Sukoyan M.A.<\/p>\n

Contamination analysis:<\/strong> bacteria, fungi and mycoplasma not detected<\/p>\n

Karyotype:<\/strong> 2n=30, XY<\/p>\n

Pluripotency:<\/strong> pluripotency is shown by embrioid body formation and teratoma formation in immunodeficient mice<\/p>\n

Additional characteristics:<\/strong><\/p>\n

Species control:<\/strong> cytogenetic<\/p>\n

Cryoconservation passage:<\/strong> 11<\/p>\n

Area of application:<\/strong> study of pluripotency, developmental biology<\/p>\n

 <\/p>\n

Source<\/strong><\/p>\n

Species:<\/strong> Neogale vison<\/em><\/p>\n

Tissue:<\/strong> morula<\/p>\n

Date:<\/strong> 01.01.1993<\/p>\n

 <\/p>\n

Cell culture<\/strong><\/p>\n

Morphology:<\/strong> mink pluripotent stem cell colonies<\/p>\n

Cell culture method:<\/strong> monolayer<\/p>\n

Cell culture medium:<\/strong> a-MEM, ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM<\/p>\n

Cell culture conditions:<\/strong> 37\u00b0C, 5% CO2<\/sub><\/p>\n

Passage protocol:<\/strong> passage with trypsin-EDTA 0.25%, split 1:3 — 1:6<\/p>\n

Cryoconservation:<\/strong> 90% KSR, 10% DMSO<\/p>\n

Cryoconservation cell concentration:<\/strong> 0.5 mln cells \/ ml<\/p>\n

Cell viability after cryoconservation:<\/strong> 70%<\/p>\n

Additional information:<\/strong> Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.<\/p>\n

\u00a0<\/strong><\/p>\n

References:<\/strong> https:\/\/doi.org\/10.1186\/1471-2164-16-S13-S6<\/a><\/p>\n

<\/a>Cell line passport of MES20<\/h2>\n

 <\/p>\n

Catalogue number:<\/strong> NVES00018<\/p>\n

Name:<\/strong> MES20<\/p>\n

Description:<\/strong> american mink (Neovison vison) embryonic stem cells derived from morula<\/p>\n

Authors:<\/strong> Matveeva N.M.<\/p>\n

Contamination analysis:<\/strong> bacteria, fungi and mycoplasma not detected<\/p>\n

Karyotype:<\/strong> 2n=30, XY, chromosome number variability 29-30, ~60, tetraploid cells 71%, modal chromosome number ~60<\/p>\n

Pluripotency:<\/strong> pluripotency is shown byteratoma formation in SCID mice<\/p>\n

Additional characteristics:<\/strong><\/p>\n

Species control:<\/strong> cytogenetic<\/p>\n

Cryoconservation passage:<\/strong> 4<\/p>\n

Area of application:<\/strong> study of pluripotency, developmental biology<\/p>\n

 <\/p>\n

Source<\/strong><\/p>\n

Species:<\/strong> Neogale vison<\/em><\/p>\n

Tissue:<\/strong> morula<\/p>\n

Date:<\/strong> 01.01.2015<\/p>\n

 <\/p>\n

Cell culture<\/strong><\/p>\n

Morphology:<\/strong> mink pluripotent stem cell colonies<\/p>\n

Cell culture method:<\/strong> monolayer<\/p>\n

Cell culture medium:<\/strong> DMEM (glucose 4.5 g\/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U\/ml<\/p>\n

Cell culture conditions:<\/strong> 37\u00b0C, 5% CO2<\/sub><\/p>\n

Passage protocol:<\/strong> passage with trypsin-EDTA 0.05%, split 1:3 — 1:6<\/p>\n

Cryoconservation:<\/strong> 90% KSR, 10% DMSO<\/p>\n

Cryoconservation cell concentration:<\/strong> 0.5 mln cells \/ ml<\/p>\n

Cell viability after cryoconservation:<\/strong> 70%<\/p>\n

Additional information:<\/strong> Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.<\/p>\n

\u00a0<\/strong><\/p>\n

References:<\/strong> https:\/\/doi.org\/10.1186\/1471-2164-16-S13-S6<\/a><\/p>\n

<\/a>Cell line passport of MES22<\/h2>\n

 <\/p>\n

Catalogue number:<\/strong> NVES00019<\/p>\n

Name:<\/strong> MES22<\/p>\n

Description:<\/strong> american mink (Neovison vison) embryonic stem cells derived from morula<\/p>\n

Authors:<\/strong> Matveeva N.M.<\/p>\n

Contamination analysis:<\/strong> bacteria, fungi and mycoplasma not detected<\/p>\n

Karyotype:<\/strong> 2n=30, XY, chromosome number variability 29-32, tetraploid cells 18%, modal chromosome number 30<\/p>\n

Pluripotency:<\/strong> pluripotency is shown byteratoma formation in SCID mice<\/p>\n

Additional characteristics:<\/strong><\/p>\n

Species control:<\/strong> cytogenetic<\/p>\n

Cryoconservation passage:<\/strong> 10<\/p>\n

Area of application:<\/strong> study of pluripotency, developmental biology<\/p>\n

 <\/p>\n

Source<\/strong><\/p>\n

Species:<\/strong> Neogale vison<\/em><\/p>\n

Tissue:<\/strong> morula<\/p>\n

Date:<\/strong> 01.01.2015<\/p>\n

 <\/p>\n

Cell culture<\/strong><\/p>\n

Morphology:<\/strong> mink pluripotent stem cell colonies<\/p>\n

Cell culture method:<\/strong> monolayer<\/p>\n

Cell culture medium:<\/strong> a-MEM, ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM<\/p>\n

Cell culture conditions:<\/strong> 37\u00b0C, 5% CO2<\/sub><\/p>\n

Passage protocol:<\/strong> passage with trypsin-EDTA 0.25%, split 1:3 — 1:6<\/p>\n

Cryoconservation:<\/strong> 90% KSR, 10% DMSO<\/p>\n

Cryoconservation cell concentration:<\/strong> 0.5 mln cells \/ ml<\/p>\n

Cell viability after cryoconservation:<\/strong> 70%<\/p>\n

Additional information:<\/strong> Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.<\/p>\n

\u00a0<\/strong><\/p>\n

References:<\/strong> https:\/\/doi.org\/10.1186\/1471-2164-16-S13-S6<\/a><\/p>\n

<\/a>Cell line passport of MES24<\/h2>\n

 <\/p>\n

Catalogue number:<\/strong> NVES00020<\/p>\n

Name:<\/strong> MES24<\/p>\n

Description:<\/strong> american mink (Neovison vison) embryonic stem cells derived from morula<\/p>\n

Authors:<\/strong> Matveeva N.M.<\/p>\n

Contamination analysis:<\/strong> bacteria, fungi and mycoplasma not detected<\/p>\n

Karyotype:<\/strong> 2n=30, XY, chromosome number variability 29-32, tetraploid cells 12%, modal chromosome number 30<\/p>\n

Pluripotency:<\/strong> pluripotency is shown byteratoma formation in SCID mice<\/p>\n

Additional characteristics:<\/strong><\/p>\n

Species control:<\/strong> cytogenetic<\/p>\n

Cryoconservation passage:<\/strong> 3<\/p>\n

Area of application:<\/strong> study of pluripotency, developmental biology<\/p>\n

 <\/p>\n

Source<\/strong><\/p>\n

Species:<\/strong> Neogale vison<\/em><\/p>\n

Tissue:<\/strong> morula<\/p>\n

Date:<\/strong> 01.01.2015<\/p>\n

 <\/p>\n

Cell culture<\/strong><\/p>\n

Morphology:<\/strong> mink pluripotent stem cell colonies<\/p>\n

Cell culture method:<\/strong> monolayer<\/p>\n

Cell culture medium:<\/strong> a-MEM, ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM<\/p>\n

Cell culture conditions:<\/strong> 37\u00b0C, 5% CO2<\/sub><\/p>\n

Passage protocol:<\/strong> passage with trypsin-EDTA 0.25%, split 1:3 — 1:6<\/p>\n

Cryoconservation:<\/strong> 90% KSR, 10% DMSO<\/p>\n

Cryoconservation cell concentration:<\/strong> 0.5 mln cells \/ ml<\/p>\n

Cell viability after cryoconservation:<\/strong> 70%<\/p>\n

Additional information:<\/strong> Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.<\/p>\n

\u00a0<\/strong><\/p>\n

References:<\/strong> https:\/\/doi.org\/10.1186\/1471-2164-16-S13-S6<\/a><\/p>\n

<\/a>Cell line passport of MES25<\/h2>\n

 <\/p>\n

Catalogue number:<\/strong> NVES00021<\/p>\n

Name:<\/strong> MES25<\/p>\n

Description:<\/strong> american mink (Neovison vison) embryonic stem cells derived from morula<\/p>\n

Authors:<\/strong> Matveeva N.M.<\/p>\n

Contamination analysis:<\/strong> bacteria, fungi and mycoplasma not detected<\/p>\n

Karyotype:<\/strong> 2n=30, XX, chromosome number variability 29-30, tetraploid cells 6%, modal chromosome number 30<\/p>\n

Pluripotency:<\/strong> pluripotency is shown by expression of specific markers<\/p>\n

Additional characteristics:<\/strong><\/p>\n

Species control:<\/strong> cytogenetic<\/p>\n

Cryoconservation passage:<\/strong> 3<\/p>\n

Area of application:<\/strong> study of pluripotency, developmental biology<\/p>\n

 <\/p>\n

Source<\/strong><\/p>\n

Species:<\/strong> Neogale vison<\/em><\/p>\n

Tissue:<\/strong> morula<\/p>\n

Date:<\/strong> 01.01.2015<\/p>\n

 <\/p>\n

Cell culture<\/strong><\/p>\n

Morphology:<\/strong> mink pluripotent stem cell colonies<\/p>\n

Cell culture method:<\/strong> monolayer<\/p>\n

Cell culture medium:<\/strong> a-MEM, ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM<\/p>\n

Cell culture conditions:<\/strong> 37\u00b0C, 5% CO2<\/sub><\/p>\n

Passage protocol:<\/strong> passage with trypsin-EDTA 0.25%, split 1:3 — 1:6<\/p>\n

Cryoconservation:<\/strong> 90% KSR, 10% DMSO<\/p>\n

Cryoconservation cell concentration:<\/strong> 0.5 mln cells \/ ml<\/p>\n

Cell viability after cryoconservation:<\/strong> 70%<\/p>\n

Additional information:<\/strong> Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.<\/p>\n

\u00a0<\/strong><\/p>\n

References:<\/strong> https:\/\/doi.org\/10.1186\/1471-2164-16-S13-S6<\/a><\/p>\n

<\/a>Cell line passport of MES27<\/h2>\n

 <\/p>\n

Catalogue number:<\/strong> NVES00022<\/p>\n

Name:<\/strong> MES27<\/p>\n

Description:<\/strong> american mink (Neovison vison) embryonic stem cells derived from morula<\/p>\n

Authors:<\/strong> Matveeva N.M.<\/p>\n

Contamination analysis:<\/strong> bacteria, fungi and mycoplasma not detected<\/p>\n

Karyotype:<\/strong> 2n=30, XY, chromosome number variability 29-32, tetraploid cells 7%, modal chromosome number 30<\/p>\n

Pluripotency:<\/strong> pluripotency is shown by expression of specific markers<\/p>\n

Additional characteristics:<\/strong><\/p>\n

Species control:<\/strong> cytogenetic<\/p>\n

Cryoconservation passage:<\/strong> 4<\/p>\n

Area of application:<\/strong> study of pluripotency, developmental biology<\/p>\n

 <\/p>\n

Source<\/strong><\/p>\n

Species:<\/strong> Neogale vison<\/em><\/p>\n

Tissue:<\/strong> morula<\/p>\n

Date:<\/strong> 01.01.2015<\/p>\n

 <\/p>\n

Cell culture<\/strong><\/p>\n

Morphology:<\/strong> mink pluripotent stem cell colonies<\/p>\n

Cell culture method:<\/strong> monolayer<\/p>\n

Cell culture medium:<\/strong> a-MEM, ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM<\/p>\n

Cell culture conditions:<\/strong> 37\u00b0C, 5% CO2<\/sub><\/p>\n

Passage protocol:<\/strong> passage with trypsin-EDTA 0.25%, split 1:3 — 1:6<\/p>\n

Cryoconservation:<\/strong> 90% KSR, 10% DMSO<\/p>\n

Cryoconservation cell concentration:<\/strong> 0.5 mln cells \/ ml<\/p>\n

Cell viability after cryoconservation:<\/strong> 70%<\/p>\n

Additional information:<\/strong> Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.<\/p>\n

\u00a0<\/strong><\/p>\n

References:<\/strong> https:\/\/doi.org\/10.1186\/1471-2164-16-S13-S6<\/a><\/p>\n

<\/a>Cell line passport of MES29<\/h2>\n

 <\/p>\n

Catalogue number:<\/strong> NVES00023<\/p>\n

Name:<\/strong> MES29<\/p>\n

Description:<\/strong> american mink (Neovison vison) embryonic stem cells derived from morula<\/p>\n

Authors:<\/strong> Matveeva N.M.<\/p>\n

Contamination analysis:<\/strong> bacteria, fungi and mycoplasma not detected<\/p>\n

Karyotype:<\/strong> 2n=30, XY, chromosome number variability 29-32, tetraploid cells 6%, modal chromosome number 30<\/p>\n

Pluripotency:<\/strong> pluripotency is shown byteratoma formation in SCID mice<\/p>\n

Additional characteristics:<\/strong><\/p>\n

Species control:<\/strong> cytogenetic<\/p>\n

Cryoconservation passage:<\/strong> 3<\/p>\n

Area of application:<\/strong> study of pluripotency, developmental biology<\/p>\n

 <\/p>\n

Source<\/strong><\/p>\n

Species:<\/strong> Neogale vison<\/em><\/p>\n

Tissue:<\/strong> morula<\/p>\n

Date:<\/strong> 01.01.2015<\/p>\n

 <\/p>\n

Cell culture<\/strong><\/p>\n

Morphology:<\/strong> mink pluripotent stem cell colonies<\/p>\n

Cell culture method:<\/strong> monolayer<\/p>\n

Cell culture medium:<\/strong> a-MEM, ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM<\/p>\n

Cell culture conditions:<\/strong> 37\u00b0C, 5% CO2<\/sub><\/p>\n

Passage protocol:<\/strong> passage with trypsin-EDTA 0.25%, split 1:3 — 1:6<\/p>\n

Cryoconservation:<\/strong> 90% KSR, 10% DMSO<\/p>\n

Cryoconservation cell concentration:<\/strong> 0.5 mln cells \/ ml<\/p>\n

Cell viability after cryoconservation:<\/strong> 70%<\/p>\n

Additional information:<\/strong> Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.<\/p>\n

\u00a0<\/strong><\/p>\n

References:<\/strong> https:\/\/doi.org\/10.1186\/1471-2164-16-S13-S6<\/a><\/p>\n

<\/a>Cell line passport of iNV1XX1<\/h2>\n

 <\/p>\n

Catalogue number:<\/strong> NVPS00066<\/p>\n

Name:<\/strong> iNV1XX1<\/p>\n

Description:<\/strong> american mink (Neovison vison) induced pluripotent stem cells derived from embryonic fibroblasts<\/p>\n

Authors:<\/strong> Menzorov A.G.<\/p>\n

Contamination analysis:<\/strong> bacteria, fungi and mycoplasma not detected<\/p>\n

Karyotype:<\/strong> 2n=30, XX, chromosome number variability 29-35, tetraploid cells 2%, modal chromosome number 30<\/p>\n

Pluripotency:<\/strong> pluripotency is shown by expression of specific markers<\/p>\n

Additional characteristics:<\/strong><\/p>\n

Species control:<\/strong> cytogenetic<\/p>\n

Cryoconservation passage:<\/strong> 3<\/p>\n

Area of application:<\/strong> study of pluripotency, developmental biology<\/p>\n

 <\/p>\n

Source<\/strong><\/p>\n

Species:<\/strong> Neogale vison<\/em><\/p>\n

Tissue:<\/strong> fibroblasts<\/p>\n

Date:<\/strong> 01.11.2017<\/p>\n

 <\/p>\n

Cell culture<\/strong><\/p>\n

Morphology:<\/strong> mink pluripotent stem cell colonies<\/p>\n

Cell culture method:<\/strong> monolayer<\/p>\n

Cell culture medium:<\/strong> a-MEM, ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM<\/p>\n

Cell culture conditions:<\/strong> 37\u00b0C, 5% CO2<\/sub><\/p>\n

Passage protocol:<\/strong> passage with trypsin-EDTA 0.25%, split 1:3 — 1:6<\/p>\n

Cryoconservation:<\/strong> 90% KSR, 10% DMSO<\/p>\n

Cryoconservation cell concentration:<\/strong> 0.5 mln cells \/ ml<\/p>\n

Cell viability after cryoconservation:<\/strong> 70%<\/p>\n

Additional information:<\/strong> Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.<\/p>\n

\u00a0<\/strong><\/p>\n

References:<\/strong> https:\/\/doi.org\/10.18699\/LettersVJ-2022-8-10<\/a><\/p>\n

<\/a>Cell line passport of iNV1XX2<\/h2>\n

 <\/p>\n

Catalogue number:<\/strong> NVPS00067<\/p>\n

Name:<\/strong> iNV1XX2<\/p>\n

Description:<\/strong> american mink (Neovison vison) induced pluripotent stem cells derived from embryonic fibroblasts<\/p>\n

Authors:<\/strong> Menzorov A.G.<\/p>\n

Contamination analysis:<\/strong> bacteria, fungi and mycoplasma not detected<\/p>\n

Karyotype:<\/strong> 2n=30, XX, chromosome number variability 29-35, tetraploid cells 4%, modal chromosome number 30<\/p>\n

Pluripotency:<\/strong> pluripotency is shown by expression of specific markers<\/p>\n

Additional characteristics:<\/strong><\/p>\n

Species control:<\/strong> cytogenetic<\/p>\n

Cryoconservation passage:<\/strong> 3<\/p>\n

Area of application:<\/strong> study of pluripotency, developmental biology<\/p>\n

 <\/p>\n

Source<\/strong><\/p>\n

Species:<\/strong> Neogale vison<\/em><\/p>\n

Tissue:<\/strong> fibroblasts<\/p>\n

Date:<\/strong> 01.11.2017<\/p>\n

 <\/p>\n

Cell culture<\/strong><\/p>\n

Morphology:<\/strong> mink pluripotent stem cell colonies<\/p>\n

Cell culture method:<\/strong> monolayer<\/p>\n

Cell culture medium:<\/strong> a-MEM, ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM<\/p>\n

Cell culture conditions:<\/strong> 37\u00b0C, 5% CO2<\/sub><\/p>\n

Passage protocol:<\/strong> passage with trypsin-EDTA 0.25%, split 1:3 — 1:6<\/p>\n

Cryoconservation:<\/strong> 90% KSR, 10% DMSO<\/p>\n

Cryoconservation cell concentration:<\/strong> 0.5 mln cells \/ ml<\/p>\n

Cell viability after cryoconservation:<\/strong> 70%<\/p>\n

Additional information:<\/strong> Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.<\/p>\n

\u00a0<\/strong><\/p>\n

References:<\/strong> https:\/\/doi.org\/10.18699\/LettersVJ-2022-8-10<\/a><\/p>\n

<\/a>Cell line passport of iNV3<\/h2>\n

 <\/p>\n

Catalogue number:<\/strong> NVPS00024<\/p>\n

Name:<\/strong> iNV3<\/p>\n

Description:<\/strong> american mink (Neovison vison) induced pluripotent stem cells derived from embryonic fibroblasts<\/p>\n

Authors:<\/strong> Menzorov A.G., Matveeva N.M., Khabarova A.A.<\/p>\n

Contamination analysis:<\/strong> bacteria, fungi and mycoplasma not detected<\/p>\n

Karyotype:<\/strong> 2n=30, XY, chromosome number variability 29-32, modal chromosome number 30<\/p>\n

Pluripotency:<\/strong> pluripotency is shown byteratoma formation in SCID mice<\/p>\n

Additional characteristics:<\/strong><\/p>\n

Species control:<\/strong> cytogenetic<\/p>\n

Cryoconservation passage:<\/strong> 3<\/p>\n

Area of application:<\/strong> study of pluripotency, developmental biology<\/p>\n

 <\/p>\n

Source<\/strong><\/p>\n

Species:<\/strong> Neogale vison<\/em><\/p>\n

Tissue:<\/strong> fibroblasts<\/p>\n

Date:<\/strong> 01.01.2015<\/p>\n

 <\/p>\n

Cell culture<\/strong><\/p>\n

Morphology:<\/strong> mink pluripotent stem cell colonies<\/p>\n

Cell culture method:<\/strong> monolayer<\/p>\n

Cell culture medium:<\/strong> a-MEM, ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM<\/p>\n

Cell culture conditions:<\/strong> 37\u00b0C, 5% CO2<\/sub><\/p>\n

Passage protocol:<\/strong> passage with trypsin-EDTA 0.25%, split 1:3 — 1:6<\/p>\n

Cryoconservation:<\/strong> 90% KSR, 10% DMSO<\/p>\n

Cryoconservation cell concentration:<\/strong> 0.5 mln cells \/ ml<\/p>\n

Cell viability after cryoconservation:<\/strong> 70%<\/p>\n

Additional information:<\/strong> Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.<\/p>\n

\u00a0<\/strong><\/p>\n

References:<\/strong> https:\/\/doi.org\/10.1186\/1471-2164-16-S13-S6<\/a><\/p>\n

<\/a>Cell line passport of iNV5<\/h2>\n

 <\/p>\n

Catalogue number:<\/strong> NVPS00025<\/p>\n

Name:<\/strong> iNV5<\/p>\n

Description:<\/strong> american mink (Neovison vison) induced pluripotent stem cells derived from embryonic fibroblasts<\/p>\n

Authors:<\/strong> Menzorov A.G., Matveeva N.M., Khabarova A.A.<\/p>\n

Contamination analysis:<\/strong> bacteria, fungi and mycoplasma not detected<\/p>\n

Karyotype:<\/strong> 2n=30, XY, chromosome number variability 29-30, tetraploid cells 7%, modal chromosome number 30<\/p>\n

Pluripotency:<\/strong> pluripotency is shown byteratoma formation in SCID mice<\/p>\n

Additional characteristics:<\/strong><\/p>\n

Species control:<\/strong> cytogenetic<\/p>\n

Cryoconservation passage:<\/strong> 3<\/p>\n

Area of application:<\/strong> study of pluripotency, developmental biology<\/p>\n

 <\/p>\n

Source<\/strong><\/p>\n

Species:<\/strong> Neogale vison<\/em><\/p>\n

Tissue:<\/strong> fibroblasts<\/p>\n

Date:<\/strong> 01.01.2015<\/p>\n

 <\/p>\n

Cell culture<\/strong><\/p>\n

Morphology:<\/strong> mink pluripotent stem cell colonies<\/p>\n

Cell culture method:<\/strong> monolayer<\/p>\n

Cell culture medium:<\/strong> a-MEM, ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM<\/p>\n

Cell culture conditions:<\/strong> 37\u00b0C, 5% CO2<\/sub><\/p>\n

Passage protocol:<\/strong> passage with trypsin-EDTA 0.25%, split 1:3 — 1:6<\/p>\n

Cryoconservation:<\/strong> 90% KSR, 10% DMSO<\/p>\n

Cryoconservation cell concentration:<\/strong> 0.5 mln cells \/ ml<\/p>\n

Cell viability after cryoconservation:<\/strong> 70%<\/p>\n

Additional information:<\/strong> Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.<\/p>\n

\u00a0<\/strong><\/p>\n

References:<\/strong> https:\/\/doi.org\/10.1186\/1471-2164-16-S13-S6<\/a><\/p>\n

<\/a>Cell line passport of iNV6<\/h2>\n

 <\/p>\n

Catalogue number:<\/strong> NVPS00026<\/p>\n

Name:<\/strong> iNV6<\/p>\n

Description:<\/strong> american mink (Neovison vison) induced pluripotent stem cells derived from embryonic fibroblasts<\/p>\n

Authors:<\/strong> Menzorov A.G., Matveeva N.M., Khabarova A.A.<\/p>\n

Contamination analysis:<\/strong> bacteria, fungi and mycoplasma not detected<\/p>\n

Karyotype:<\/strong> 2n=30, XY, chromosome number variability 29-32, tetraploid cells 7%, modal chromosome number 30<\/p>\n

Pluripotency:<\/strong> pluripotency is shown byteratoma formation in SCID mice<\/p>\n

Additional characteristics:<\/strong><\/p>\n

Species control:<\/strong> cytogenetic<\/p>\n

Cryoconservation passage:<\/strong> 6<\/p>\n

Area of application:<\/strong> study of pluripotency, developmental biology<\/p>\n

 <\/p>\n

Source<\/strong><\/p>\n

Species:<\/strong> Neogale vison<\/em><\/p>\n

Tissue:<\/strong> fibroblasts<\/p>\n

Date:<\/strong> 01.01.2015<\/p>\n

 <\/p>\n

Cell culture<\/strong><\/p>\n

Morphology:<\/strong> mink pluripotent stem cell colonies<\/p>\n

Cell culture method:<\/strong> monolayer<\/p>\n

Cell culture medium:<\/strong> a-MEM, ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM<\/p>\n

Cell culture conditions:<\/strong> 37\u00b0C, 5% CO2<\/sub><\/p>\n

Passage protocol:<\/strong> passage with trypsin-EDTA 0.25%, split 1:3 — 1:6<\/p>\n

Cryoconservation:<\/strong> 90% KSR, 10% DMSO<\/p>\n

Cryoconservation cell concentration:<\/strong> 0.5 mln cells \/ ml<\/p>\n

Cell viability after cryoconservation:<\/strong> 70%<\/p>\n

Additional information:<\/strong> Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.<\/p>\n

\u00a0<\/strong><\/p>\n

References:<\/strong> https:\/\/doi.org\/10.1186\/1471-2164-16-S13-S6<\/a><\/p>\n

<\/a>Cell line passport of iNV7<\/h2>\n

 <\/p>\n

Catalogue number:<\/strong> NVPS00027<\/p>\n

Name:<\/strong> iNV7<\/p>\n

Description:<\/strong> american mink (Neovison vison) induced pluripotent stem cells derived from embryonic fibroblasts<\/p>\n

Authors:<\/strong> Menzorov A.G., Matveeva N.M., Khabarova A.A.<\/p>\n

Contamination analysis:<\/strong> bacteria, fungi and mycoplasma not detected<\/p>\n

Karyotype:<\/strong> 2n=30, XY, chromosome number variability 30-32, tetraploid cells 10%, modal chromosome number 30<\/p>\n

Pluripotency:<\/strong> pluripotency is shown byteratoma formation in SCID mice<\/p>\n

Additional characteristics:<\/strong><\/p>\n

Species control:<\/strong> cytogenetic<\/p>\n

Cryoconservation passage:<\/strong> 10<\/p>\n

Area of application:<\/strong> study of pluripotency, developmental biology<\/p>\n

 <\/p>\n

Source<\/strong><\/p>\n

Species:<\/strong> Neogale vison<\/em><\/p>\n

Tissue:<\/strong> fibroblasts<\/p>\n

Date:<\/strong> 01.01.2015<\/p>\n

 <\/p>\n

Cell culture<\/strong><\/p>\n

Morphology:<\/strong> mink pluripotent stem cell colonies<\/p>\n

Cell culture method:<\/strong> monolayer<\/p>\n

Cell culture medium:<\/strong> a-MEM, ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM<\/p>\n

Cell culture conditions:<\/strong> 37\u00b0C, 5% CO2<\/sub><\/p>\n

Passage protocol:<\/strong> passage with trypsin-EDTA 0.25%, split 1:3 — 1:6<\/p>\n

Cryoconservation:<\/strong> 90% KSR, 10% DMSO<\/p>\n

Cryoconservation cell concentration:<\/strong> 0.5 mln cells \/ ml<\/p>\n

Cell viability after cryoconservation:<\/strong> 70%<\/p>\n

Additional information:<\/strong> Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.<\/p>\n

\u00a0<\/strong><\/p>\n

References:<\/strong> https:\/\/doi.org\/10.1186\/1471-2164-16-S13-S6<\/a><\/p>\n

<\/a>Cell line passport of iNV9<\/h2>\n

 <\/p>\n

Catalogue number:<\/strong> NVPS00028<\/p>\n

Name:<\/strong> iNV9<\/p>\n

Description:<\/strong> american mink (Neovison vison) induced pluripotent stem cells derived from embryonic fibroblasts<\/p>\n

Authors:<\/strong> Menzorov A.G., Matveeva N.M., Khabarova A.A.<\/p>\n

Contamination analysis:<\/strong> bacteria, fungi and mycoplasma not detected<\/p>\n

Karyotype:<\/strong> 2n=30, XY, chromosome number variability 29-30, tetraploid cells 9%, modal chromosome number 30<\/p>\n

Pluripotency:<\/strong> pluripotency is shown by expression of specific markers<\/p>\n

Additional characteristics:<\/strong><\/p>\n

Species control:<\/strong> cytogenetic<\/p>\n

Cryoconservation passage:<\/strong> 12<\/p>\n

Area of application:<\/strong> study of pluripotency, developmental biology<\/p>\n

 <\/p>\n

Source<\/strong><\/p>\n

Species:<\/strong> Neogale vison<\/em><\/p>\n

Tissue:<\/strong> fibroblasts<\/p>\n

Date:<\/strong> 01.01.2015<\/p>\n

 <\/p>\n

Cell culture<\/strong><\/p>\n

Morphology:<\/strong> mink pluripotent stem cell colonies<\/p>\n

Cell culture method:<\/strong> monolayer<\/p>\n

Cell culture medium:<\/strong> a-MEM, ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM<\/p>\n

Cell culture conditions:<\/strong> 37\u00b0C, 5% CO2<\/sub><\/p>\n

Passage protocol:<\/strong> passage with trypsin-EDTA 0.25%, split 1:3 — 1:6<\/p>\n

Cryoconservation:<\/strong> 90% KSR, 10% DMSO<\/p>\n

Cryoconservation cell concentration:<\/strong> 0.5 mln cells \/ ml<\/p>\n

Cell viability after cryoconservation:<\/strong> 70%<\/p>\n

Additional information:<\/strong> Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.<\/p>\n

\u00a0<\/strong><\/p>\n

References:<\/strong> https:\/\/doi.org\/10.1186\/1471-2164-16-S13-S6<\/a><\/p>\n

<\/a>Cell line passport of iNV11<\/h2>\n

 <\/p>\n

Catalogue number:<\/strong> NVPS00029<\/p>\n

Name:<\/strong> iNV11<\/p>\n

Description:<\/strong> american mink (Neovison vison) induced pluripotent stem cells derived from embryonic fibroblasts<\/p>\n

Authors:<\/strong> Menzorov A.G., Matveeva N.M., Khabarova A.A.<\/p>\n

Contamination analysis:<\/strong> bacteria, fungi and mycoplasma not detected<\/p>\n

Karyotype:<\/strong> 2n=30, XY, chromosome number variability 29-32, tetraploid cells 9%, modal chromosome number 30<\/p>\n

Pluripotency:<\/strong> pluripotency is shown byteratoma formation in SCID mice<\/p>\n

Additional characteristics:<\/strong><\/p>\n

Species control:<\/strong> cytogenetic<\/p>\n

Cryoconservation passage:<\/strong> 11<\/p>\n

Area of application:<\/strong> study of pluripotency, developmental biology<\/p>\n

 <\/p>\n

Source<\/strong><\/p>\n

Species:<\/strong> Neogale vison<\/em><\/p>\n

Tissue:<\/strong> fibroblasts<\/p>\n

Date:<\/strong> 01.01.2015<\/p>\n

 <\/p>\n

Cell culture<\/strong><\/p>\n

Morphology:<\/strong> mink pluripotent stem cell colonies<\/p>\n

Cell culture method:<\/strong> monolayer<\/p>\n

Cell culture medium:<\/strong> a-MEM, ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM<\/p>\n

Cell culture conditions:<\/strong> 37\u00b0C, 5% CO2<\/sub><\/p>\n

Passage protocol:<\/strong> passage with trypsin-EDTA 0.25%, split 1:3 — 1:6<\/p>\n

Cryoconservation:<\/strong> 90% KSR, 10% DMSO<\/p>\n

Cryoconservation cell concentration:<\/strong> 0.5 mln cells \/ ml<\/p>\n

Cell viability after cryoconservation:<\/strong> 70%<\/p>\n

Additional information:<\/strong> Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.<\/p>\n

\u00a0<\/strong><\/p>\n

References:<\/strong> https:\/\/doi.org\/10.1186\/1471-2164-16-S13-S6<\/a><\/p>\n

<\/a>Cell line passport of iNV13<\/h2>\n

 <\/p>\n

Catalogue number:<\/strong> NVPS00030<\/p>\n

Name:<\/strong> iNV13<\/p>\n

Description:<\/strong> american mink (Neovison vison) induced pluripotent stem cells derived from embryonic fibroblasts<\/p>\n

Authors:<\/strong> Menzorov A.G., Matveeva N.M., Khabarova A.A.<\/p>\n

Contamination analysis:<\/strong> bacteria, fungi and mycoplasma not detected<\/p>\n

Karyotype:<\/strong> 2n=30, XY, chromosome number variability 29-30, tetraploid cells 3%, modal chromosome number 30<\/p>\n

Pluripotency:<\/strong> pluripotency is shown byteratoma formation in SCID mice<\/p>\n

Additional characteristics:<\/strong><\/p>\n

Species control:<\/strong> cytogenetic<\/p>\n

Cryoconservation passage:<\/strong> 3<\/p>\n

Area of application:<\/strong> study of pluripotency, developmental biology<\/p>\n

 <\/p>\n

Source<\/strong><\/p>\n

Species:<\/strong> Neogale vison<\/em><\/p>\n

Tissue:<\/strong> fibroblasts<\/p>\n

Date:<\/strong> 01.01.2015<\/p>\n

 <\/p>\n

Cell culture<\/strong><\/p>\n

Morphology:<\/strong> mink pluripotent stem cell colonies<\/p>\n

Cell culture method:<\/strong> monolayer<\/p>\n

Cell culture medium:<\/strong> a-MEM, ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM<\/p>\n

Cell culture conditions:<\/strong> 37\u00b0C, 5% CO2<\/sub><\/p>\n

Passage protocol:<\/strong> passage with trypsin-EDTA 0.25%, split 1:3 — 1:6<\/p>\n

Cryoconservation:<\/strong> 90% KSR, 10% DMSO<\/p>\n

Cryoconservation cell concentration:<\/strong> 0.5 mln cells \/ ml<\/p>\n

Cell viability after cryoconservation:<\/strong> 70%<\/p>\n

Additional information:<\/strong> Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.<\/p>\n

\u00a0<\/strong><\/p>\n

References:<\/strong> https:\/\/doi.org\/10.1186\/1471-2164-16-S13-S6<\/a><\/p>\n

<\/a>Cell line passport of iNV15<\/h2>\n

 <\/p>\n

Catalogue number:<\/strong> NVPS00031<\/p>\n

Name:<\/strong> iNV15<\/p>\n

Description:<\/strong> american mink (Neovison vison) induced pluripotent stem cells derived from embryonic fibroblasts<\/p>\n

Authors:<\/strong> Menzorov A.G., Matveeva N.M., Khabarova A.A.<\/p>\n

Contamination analysis:<\/strong> bacteria, fungi and mycoplasma not detected<\/p>\n

Karyotype:<\/strong> 2n=30, XY, chromosome number variability 29-32, tetraploid cells 6%, modal chromosome number 30<\/p>\n

Pluripotency:<\/strong> pluripotency is shown byteratoma formation in SCID mice<\/p>\n

Additional characteristics:<\/strong><\/p>\n

Species control:<\/strong> cytogenetic<\/p>\n

Cryoconservation passage:<\/strong> 3<\/p>\n

Area of application:<\/strong> study of pluripotency, developmental biology<\/p>\n

 <\/p>\n

Source<\/strong><\/p>\n

Species:<\/strong> Neogale vison<\/em><\/p>\n

Tissue:<\/strong> fibroblasts<\/p>\n

Date:<\/strong> 01.01.2015<\/p>\n

 <\/p>\n

Cell culture<\/strong><\/p>\n

Morphology:<\/strong> mink pluripotent stem cell colonies<\/p>\n

Cell culture method:<\/strong> monolayer<\/p>\n

Cell culture medium:<\/strong> a-MEM, ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM<\/p>\n

Cell culture conditions:<\/strong> 37\u00b0C, 5% CO2<\/sub><\/p>\n

Passage protocol:<\/strong> passage with trypsin-EDTA 0.25%, split 1:3 — 1:6<\/p>\n

Cryoconservation:<\/strong> 90% KSR, 10% DMSO<\/p>\n

Cryoconservation cell concentration:<\/strong> 0.5 mln cells \/ ml<\/p>\n

Cell viability after cryoconservation:<\/strong> 70%<\/p>\n

Additional information:<\/strong> Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.<\/p>\n

\u00a0<\/strong><\/p>\n

References:<\/strong> https:\/\/doi.org\/10.1186\/1471-2164-16-S13-S6<\/a><\/p>\n

<\/a>Cell line passport of iNV18<\/h2>\n

 <\/p>\n

Catalogue number:<\/strong> NVPS00032<\/p>\n

Name:<\/strong> iNV18<\/p>\n

Description:<\/strong> american mink (Neovison vison) induced pluripotent stem cells derived from embryonic fibroblasts<\/p>\n

Authors:<\/strong> Menzorov A.G., Matveeva N.M., Khabarova A.A.<\/p>\n

Contamination analysis:<\/strong> bacteria, fungi and mycoplasma not detected<\/p>\n

Karyotype:<\/strong> 2n=30, XY, chromosome number variability 29-32, tetraploid cells 8%, modal chromosome number 30<\/p>\n

Pluripotency:<\/strong> pluripotency is shown by expression of specific markers<\/p>\n

Additional characteristics:<\/strong><\/p>\n

Species control:<\/strong> cytogenetic<\/p>\n

Cryoconservation passage:<\/strong> 6<\/p>\n

Area of application:<\/strong> study of pluripotency, developmental biology<\/p>\n

 <\/p>\n

Source<\/strong><\/p>\n

Species:<\/strong> Neogale vison<\/em><\/p>\n

Tissue:<\/strong> fibroblasts<\/p>\n

Date:<\/strong> 01.01.2015<\/p>\n

 <\/p>\n

Cell culture<\/strong><\/p>\n

Morphology:<\/strong> mink pluripotent stem cell colonies<\/p>\n

Cell culture method:<\/strong> monolayer<\/p>\n

Cell culture medium:<\/strong> a-MEM, ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM<\/p>\n

Cell culture conditions:<\/strong> 37\u00b0C, 5% CO2<\/sub><\/p>\n

Passage protocol:<\/strong> passage with trypsin-EDTA 0.25%, split 1:3 — 1:6<\/p>\n

Cryoconservation:<\/strong> 90% KSR, 10% DMSO<\/p>\n

Cryoconservation cell concentration:<\/strong> 0.5 mln cells \/ ml<\/p>\n

Cell viability after cryoconservation:<\/strong> 70%<\/p>\n

Additional information:<\/strong> Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.<\/p>\n

\u00a0<\/strong><\/p>\n

References:<\/strong> https:\/\/doi.org\/10.1186\/1471-2164-16-S13-S6<\/a><\/p>\n

<\/a>Cell line passport of iNV19<\/h2>\n

 <\/p>\n

Catalogue number:<\/strong> NVPS00033<\/p>\n

Name:<\/strong> iNV19<\/p>\n

Description:<\/strong> american mink (Neovison vison) induced pluripotent stem cells derived from embryonic fibroblasts<\/p>\n

Authors:<\/strong> Menzorov A.G., Matveeva N.M., Khabarova A.A.<\/p>\n

Contamination analysis:<\/strong> bacteria, fungi and mycoplasma not detected<\/p>\n

Karyotype:<\/strong> 2n=30, XY, chromosome number variability 29-30, tetraploid cells 8%, modal chromosome number 30<\/p>\n

Pluripotency:<\/strong> pluripotency is shown byteratoma formation in SCID mice<\/p>\n

Additional characteristics:<\/strong><\/p>\n

Species control:<\/strong> cytogenetic<\/p>\n

Cryoconservation passage:<\/strong> 4<\/p>\n

Area of application:<\/strong> study of pluripotency, developmental biology<\/p>\n

 <\/p>\n

Source<\/strong><\/p>\n

Species:<\/strong> Neogale vison<\/em><\/p>\n

Tissue:<\/strong> fibroblasts<\/p>\n

Date:<\/strong> 01.01.2015<\/p>\n

 <\/p>\n

Cell culture<\/strong><\/p>\n

Morphology:<\/strong> mink pluripotent stem cell colonies<\/p>\n

Cell culture method:<\/strong> monolayer<\/p>\n

Cell culture medium:<\/strong> a-MEM, ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM<\/p>\n

Cell culture conditions:<\/strong> 37\u00b0C, 5% CO2<\/sub><\/p>\n

Passage protocol:<\/strong> passage with trypsin-EDTA 0.25%, split 1:3 — 1:6<\/p>\n

Cryoconservation:<\/strong> 90% KSR, 10% DMSO<\/p>\n

Cryoconservation cell concentration:<\/strong> 0.5 mln cells \/ ml<\/p>\n

Cell viability after cryoconservation:<\/strong> 70%<\/p>\n

Additional information:<\/strong> Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.<\/p>\n

\u00a0<\/strong><\/p>\n

References:<\/strong> https:\/\/doi.org\/10.1186\/1471-2164-16-S13-S6<\/a><\/p>\n

<\/a>Cell line passport of iNV20<\/h2>\n

 <\/p>\n

Catalogue number:<\/strong> NVPS00034<\/p>\n

Name:<\/strong> iNV20<\/p>\n

Description:<\/strong> american mink (Neovison vison) induced pluripotent stem cells derived from embryonic fibroblasts<\/p>\n

Authors:<\/strong> Menzorov A.G., Matveeva N.M., Khabarova A.A.<\/p>\n

Contamination analysis:<\/strong> bacteria, fungi and mycoplasma not detected<\/p>\n

Karyotype:<\/strong> 2n=30, XY, chromosome number variability 29-32, tetraploid cells 21%, modal chromosome number 30<\/p>\n

Pluripotency:<\/strong> pluripotency is shown byteratoma formation in SCID mice<\/p>\n

Additional characteristics:<\/strong><\/p>\n

Species control:<\/strong> cytogenetic<\/p>\n

Cryoconservation passage:<\/strong> 3<\/p>\n

Area of application:<\/strong> study of pluripotency, developmental biology<\/p>\n

 <\/p>\n

Source<\/strong><\/p>\n

Species:<\/strong> Neogale vison<\/em><\/p>\n

Tissue:<\/strong> fibroblasts<\/p>\n

Date:<\/strong> 01.01.2015<\/p>\n

 <\/p>\n

Cell culture<\/strong><\/p>\n

Morphology:<\/strong> mink pluripotent stem cell colonies<\/p>\n

Cell culture method:<\/strong> monolayer<\/p>\n

Cell culture medium:<\/strong> a-MEM, ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM<\/p>\n

Cell culture conditions:<\/strong> 37\u00b0C, 5% CO2<\/sub><\/p>\n

Passage protocol:<\/strong> passage with trypsin-EDTA 0.25%, split 1:3 — 1:6<\/p>\n

Cryoconservation:<\/strong> 90% KSR, 10% DMSO<\/p>\n

Cryoconservation cell concentration:<\/strong> 0.5 mln cells \/ ml<\/p>\n

Cell viability after cryoconservation:<\/strong> 70%<\/p>\n

Additional information:<\/strong> Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.<\/p>\n

\u00a0<\/strong><\/p>\n

References:<\/strong> https:\/\/doi.org\/10.1186\/1471-2164-16-S13-S6<\/a><\/p>\n

<\/h2>\n

<\/a><\/a>Immortalised cell lines<\/h1>\n

<\/a><\/a>Cell line passport of CHO-hCNTN6-HA<\/h2>\n

 <\/p>\n

Catalogue number: <\/strong>CGOC00103<\/p>\n

Name: <\/strong>CHO-hCNTN6-HA<\/p>\n

Description: <\/strong>genetically modified CHO cell line (Chinese hamster ovary cells, Cricetulus griseus<\/em>) with a constitutive expression of the human CNTN6<\/em> gene and HA-tag<\/p>\n

Authors: <\/strong>Yunusova A.M., Chvileva A.S., Shnaider T.A.<\/p>\n

Contamination analysis: <\/strong>bacteria, fungi and mycoplasma not detected<\/p>\n

Karyotype:<\/strong> 2n = 22, chromosome number variability 16-20, modal chromosome number 19, polyploid cells 13%<\/p>\n

Pluripotency:<\/strong><\/p>\n

Additional characteristics:<\/strong><\/p>\n

Species control:<\/strong> cytogenetic<\/p>\n

Cryoconservation passage:<\/strong> 22<\/p>\n

Area of application:<\/strong> Notch signaling pathway study<\/p>\n

 <\/p>\n

Source<\/strong><\/p>\n

Species:<\/strong> Cricetulus griseus<\/em><\/p>\n

Tissue:<\/strong> ovary<\/p>\n

Date:<\/strong> 09.10.2024<\/p>\n

 <\/p>\n

Cell culture<\/strong><\/p>\n

Morphology:<\/strong> epithelial-like cells<\/p>\n

Cell culture method:<\/strong> monolayer<\/p>\n

Cell culture medium:<\/strong> DMEM\/F12, FBS 10%, PenStrep 1%<\/p>\n

Cell culture conditions:<\/strong> 37\u00b0C, 5% CO2<\/sub><\/p>\n

Passage protocol: <\/strong>cell passage with 0.25% Trypsin-EDTA at the ratio 1:3 — 1:10<\/p>\n

Cryoconservation: <\/strong>50% FBS, 40% DMEM\/F12, 10% DMSO<\/p>\n

Cryoconservation cell concentration: <\/strong>0.5 mln cells \/ ml<\/p>\n

Cell viability after cryoconservation: <\/strong>90%<\/p>\n

Additional information:<\/strong><\/p>\n

\u00a0<\/strong><\/p>\n

References:<\/strong><\/p>\n

 <\/p>\n

 <\/p>\n

<\/a><\/a>Cell line passport of CHO-hDLL1-HA<\/h2>\n

 <\/p>\n

Catalogue number: <\/strong>CGOC00104<\/p>\n

Name: <\/strong>CHO-hDLL1-HA<\/p>\n

Description: <\/strong>genetically modified CHO cell line (Chinese hamster ovary cells, Cricetulus griseus<\/em>) with a constitutive expression of the human DLL1<\/em> gene and HA-tag<\/p>\n

Authors: <\/strong>Yunusova A.M., Chvileva A.S., Shnaider T.A.<\/p>\n

Contamination analysis: <\/strong>bacteria, fungi and mycoplasma not detected<\/p>\n

Karyotype:<\/strong> 2n = 22, chromosome number variability 19-22, modal chromosome number 20, polyploid cells 9%<\/p>\n

Pluripotency:<\/strong><\/p>\n

Additional characteristics:<\/strong><\/p>\n

Species control:<\/strong> cytogenetic<\/p>\n

Cryoconservation passage:<\/strong> 20<\/p>\n

Area of application:<\/strong> Notch signaling pathway study<\/p>\n

 <\/p>\n

Source<\/strong><\/p>\n

Species:<\/strong> Cricetulus griseus<\/em><\/p>\n

Tissue:<\/strong> ovary<\/p>\n

Date:<\/strong> 09.10.2024<\/p>\n

 <\/p>\n

Cell culture<\/strong><\/p>\n

Morphology:<\/strong> epithelial-like cells<\/p>\n

Cell culture method:<\/strong> monolayer<\/p>\n

Cell culture medium:<\/strong> DMEM\/F12, FBS 10%, PenStrep 1%<\/p>\n

Cell culture conditions:<\/strong> 37\u00b0C, 5% CO2<\/sub><\/p>\n

Passage protocol: <\/strong>cell passage with 0.25% Trypsin-EDTA at the ratio 1:3 — 1:10<\/p>\n

Cryoconservation: <\/strong>50% FBS, 40% DMEM\/F12, 10% DMSO<\/p>\n

Cryoconservation cell concentration: <\/strong>0.5 mln cells \/ ml<\/p>\n

Cell viability after cryoconservation: <\/strong>90%<\/p>\n

Additional information:<\/strong><\/p>\n

\u00a0<\/strong><\/p>\n

References:<\/strong><\/p>\n

 <\/p>\n

 <\/p>\n

<\/a><\/a>Cell line passport of CHO-hNOTCH1-FLAG<\/h2>\n

 <\/p>\n

Catalogue number: <\/strong>CGOC00105<\/p>\n

Name: <\/strong>CHO-hNOTCH1-FLAG<\/p>\n

Description: <\/strong>genetically modified CHO cell line (Chinese hamster ovary cells, Cricetulus griseus<\/em>) with a constitutive expression of the human NOTCH1<\/em> gene and FLAG-tag<\/p>\n

Authors: <\/strong>Yunusova A.M., Chvileva A.S., Shnaider T.A.<\/p>\n

Contamination analysis: <\/strong>bacteria, fungi and mycoplasma not detected<\/p>\n

Karyotype:<\/strong> 2n = 22, chromosome number variability 18-21, modal chromosome number 20, polyploid cells 12%<\/p>\n

Pluripotency:<\/strong><\/p>\n

Additional characteristics:<\/strong><\/p>\n

Species control:<\/strong> cytogenetic<\/p>\n

Cryoconservation passage:<\/strong> 23<\/p>\n

Area of application:<\/strong> Notch signaling pathway study<\/p>\n

 <\/p>\n

Source<\/strong><\/p>\n

Species:<\/strong> Cricetulus griseus<\/em><\/p>\n

Tissue:<\/strong> ovary<\/p>\n

Date:<\/strong> 09.10.2024<\/p>\n

 <\/p>\n

Cell culture<\/strong><\/p>\n

Morphology:<\/strong> epithelial-like cells<\/p>\n

Cell culture method:<\/strong> monolayer<\/p>\n

Cell culture medium:<\/strong> DMEM\/F12, FBS 10%, PenStrep 1%<\/p>\n

Cell culture conditions:<\/strong> 37\u00b0C, 5% CO2<\/sub><\/p>\n

Passage protocol: <\/strong>cell passage with 0.25% Trypsin-EDTA at the ratio 1:3 — 1:10<\/p>\n

Cryoconservation: <\/strong>50% FBS, 40% DMEM\/F12, 10% DMSO<\/p>\n

Cryoconservation cell concentration: <\/strong>0.5 mln cells \/ ml<\/p>\n

Cell viability after cryoconservation: <\/strong>90%<\/p>\n

Additional information:<\/strong><\/p>\n

\u00a0<\/strong><\/p>\n

References:<\/strong><\/p>\n

 <\/p>\n

<\/a><\/a>Cell line passport of CHO-hNOTCH2-FLAG<\/h2>\n

 <\/p>\n

Catalogue number: <\/strong>CGOC00106<\/p>\n

Name: <\/strong>CHO-hNOTCH2-FLAG<\/p>\n

Description: <\/strong>genetically modified CHO cell line (Chinese hamster ovary cells, Cricetulus griseus<\/em>) with a constitutive expression of the human NOTCH2<\/em> gene and FLAG-tag<\/p>\n

Authors: <\/strong>Yunusova A.M., Chvileva A.S., Shnaider T.A.<\/p>\n

Contamination analysis: <\/strong>bacteria, fungi and mycoplasma not detected<\/p>\n

Karyotype:<\/strong> 2n = 22, chromosome number variability 20-21, modal chromosome number 21, polyploid cells 15%<\/p>\n

Pluripotency:<\/strong><\/p>\n

Additional characteristics:<\/strong><\/p>\n

Species control:<\/strong> cytogenetic<\/p>\n

Cryoconservation passage:<\/strong> 23<\/p>\n

Area of application:<\/strong> Notch signaling pathway study<\/p>\n

 <\/p>\n

Source<\/strong><\/p>\n

Species:<\/strong> Cricetulus griseus<\/em><\/p>\n

Tissue:<\/strong> ovary<\/p>\n

Date:<\/strong> 09.10.2024<\/p>\n

 <\/p>\n

Cell culture<\/strong><\/p>\n

Morphology:<\/strong> epithelial-like cells<\/p>\n

Cell culture method:<\/strong> monolayer<\/p>\n

Cell culture medium:<\/strong> DMEM\/F12, FBS 10%, PenStrep 1%<\/p>\n

Cell culture conditions:<\/strong> 37\u00b0C, 5% CO2<\/sub><\/p>\n

Passage protocol: <\/strong>cell passage with 0.25% Trypsin-EDTA at the ratio 1:3 — 1:10<\/p>\n

Cryoconservation: <\/strong>50% FBS, 40% DMEM\/F12, 10% DMSO<\/p>\n

Cryoconservation cell concentration: <\/strong>0.5 mln cells \/ ml<\/p>\n

Cell viability after cryoconservation: <\/strong>90%<\/p>\n

Additional information:<\/strong><\/p>\n

\u00a0<\/strong><\/p>\n

References:<\/strong><\/p>\n

<\/a><\/a>Bird fibroblasts<\/h1>\n

<\/a><\/a>Cell line passport of OFC1A<\/h2>\n

 <\/p>\n

Catalogue number: <\/strong>PMOF00097<\/p>\n

Name: <\/strong>OFC1A<\/p>\n

Description: <\/strong>Great tit ovary cells with fibroblast morphology<\/p>\n

Authors: <\/strong>Pristyazhnyuk I.E., Malinovskaya L.P.<\/p>\n

Contamination analysis: <\/strong>bacteria, fungi and mycoplasma not detected<\/p>\n

Karyotype:<\/strong> 2N,ZW, 6 pairs of macrochromosomes, 33 pairs of microchromosomes<\/p>\n

Pluripotency:<\/strong><\/p>\n

Additional characteristics:<\/strong> presence of rearranged chromosome homologues to chromosomes 4, 5 or 6.<\/p>\n

Species control:<\/strong> cytogenetic<\/p>\n

Cryoconservation passage:<\/strong> 8<\/p>\n

Area of application:<\/strong><\/p>\n

 <\/p>\n

Source<\/strong><\/p>\n

Species:<\/strong> Parus major<\/em><\/p>\n

Tissue:<\/strong> ovary<\/p>\n

Date:<\/strong> 26.08.2022<\/p>\n

 <\/p>\n

Cell culture<\/strong><\/p>\n

Morphology:<\/strong> fibroblast-like cells<\/p>\n

Cell culture method:<\/strong> monolayer<\/p>\n

Cell culture medium:<\/strong> DMEM, FBS 10%, 2% chicken serum, NEAA 1%, Glutamine 1%, PenStrep 1%<\/p>\n

Cell culture conditions:<\/strong> 37\u00b0C, 5% CO2<\/sub><\/p>\n

Passage protocol: <\/strong>cell passage with Trypsin-EDTA at the ratio 1:3<\/p>\n

Cryoconservation: <\/strong>50% FBS, 40% DMEM, 10% DMSO<\/p>\n

Cryoconservation cell concentration: <\/strong>0.5 mln cells \/ ml<\/p>\n

Cell viability after cryoconservation: <\/strong>70%<\/p>\n

Additional information: <\/strong><\/p>\n

\u00a0<\/strong><\/p>\n

References:<\/strong> https:\/\/doi.org\/10.3390\/ani12131724<\/a><\/p>\n

 <\/p>\n

\u00a0<\/strong><\/p>\n

<\/a>Protists<\/h1>\n

<\/a><\/a>Cell line passport of THAU1<\/h2>\n

 <\/p>\n

Catalogue number: <\/strong>TAHE00071<\/p>\n

Name: <\/strong>THAU1<\/p>\n

Description: <\/strong>the protist Thraustochytrium aureum<\/em> ssp. strugatskii<\/em> was isolated from the dissociated comb jelly Beroe ovata<\/em> (from the Black Sea)<\/p>\n

Authors: <\/strong>Menzorov A.G., Doroshkov A.V.<\/p>\n

Contamination analysis: <\/strong>bacteria, fungi and mycoplasma not detected<\/p>\n

Karyotype:<\/strong><\/p>\n

Pluripotency:<\/strong><\/p>\n

Additional characteristics: <\/strong><\/p>\n

Species control: <\/strong>rRNA sequencing<\/p>\n

Cryoconservation passage: <\/strong>10<\/p>\n

Area of application: <\/strong>biotechnology, fatty acid production, study of the Labyrinthulea life cycle<\/p>\n

 <\/p>\n

Source<\/strong><\/p>\n

Species: <\/strong>Thraustochytrium aureum<\/em> ssp. strugatskii<\/em><\/p>\n

Tissue:<\/strong><\/p>\n

Date:<\/strong> 26.11.2020<\/p>\n

 <\/p>\n

Cell culture<\/strong><\/p>\n

Morphology:<\/strong> \u201ccolonies\u201d of cells<\/p>\n

Cell culture method: <\/strong>monolayer<\/p>\n

Cell culture medium: <\/strong>a) FAND culture medium: 17 ASW, 5% FBS,<\/p>\n

5% DMEM (prepared from powder on 17\u2030 ASW), x0.05 NEAA, x1 PenStrep; b) 790 By+ (ATCC)<\/p>\n

Cell culture conditions:<\/strong> room temperature<\/p>\n

Passage protocol: <\/strong>manual passage (scraping and resuspending) at the ratio 1:10 — 1:100<\/p>\n

Cryoconservation: <\/strong>90% FBS, 10% DMSO<\/p>\n

Cryoconservation cell concentration: <\/strong>1 mln cells \/ ml<\/p>\n

Cell viability after cryoconservation: <\/strong>70%<\/p>\n

Additional information:<\/strong><\/p>\n

\u00a0<\/strong><\/p>\n

References:<\/strong> http:\/\/dx.doi.org\/10.7717\/peerj.12737<\/a><\/p>\n

 <\/p>\n

<\/a><\/a>Cell line passport of THCA1<\/h2>\n

 <\/p>\n

Catalogue number: <\/strong>TAHE00107<\/p>\n

Name: <\/strong>THCA1<\/p>\n

Description: <\/strong>the protist Thraustochytrium caudivorum<\/em> was isolated from the biota of the free-living flatworm Macrostomum lignano<\/em><\/p>\n

Authors: <\/strong>Menzorov A.G., Biryukov M.Yu.<\/p>\n

Contamination analysis: <\/strong>bacteria, fungi and mycoplasma not detected<\/p>\n

Karyotype:<\/strong><\/p>\n

Pluripotency:<\/strong><\/p>\n

Additional characteristics: <\/strong><\/p>\n

Species control: <\/strong>rRNA sequencing (NCBI Genbank PV862890.1 and PV862891.1)<\/p>\n

Cryoconservation passage: <\/strong>12<\/p>\n

Area of application: <\/strong>study of host-parasite interactions, study of the life cycle of Labyrinthulea<\/p>\n

 <\/p>\n

Source<\/strong><\/p>\n

Species: <\/strong>Thraustochytrium caudivorum<\/em><\/p>\n

Tissue:<\/strong><\/p>\n

Date:<\/strong> 22.07.2025<\/p>\n

 <\/p>\n

Cell culture<\/strong><\/p>\n

Morphology:<\/strong> single cells<\/p>\n

Cell culture method: <\/strong>monolayer<\/p>\n

Cell culture medium: <\/strong>790 By+ (ATCC)<\/p>\n

Cell culture conditions:<\/strong> room temperature<\/p>\n

Passage protocol: <\/strong>manual passage (scraping and resuspending) at the ratio 1:2 — 1:5<\/p>\n

Cryoconservation: <\/strong>90% FBS, 10% DMSO<\/p>\n

Cryoconservation cell concentration: <\/strong>1 mln cells \/ ml<\/p>\n

Cell viability after cryoconservation: <\/strong>70%<\/p>\n

Additional information:<\/strong><\/p>\n

\u00a0<\/strong><\/p>\n

References:<\/strong><\/p>\n

 <\/p>\n

<\/a>Cell line passport of THKI1<\/h2>\n

 <\/p>\n

Catalogue number: <\/strong>TAHE00108<\/p>\n

Name: <\/strong>THKI1<\/p>\n

Description: <\/strong>the protist Thraustochytrium kinnei<\/em> was isolated from the dissociated comb jelly Beroe ovata<\/em> (from the Black Sea)<\/p>\n

Authors: <\/strong>Menzorov A.G., Doroshkov A.V.<\/p>\n

Contamination analysis: <\/strong>bacteria, fungi and mycoplasma not detected<\/p>\n

Karyotype:<\/strong><\/p>\n

Pluripotency:<\/strong><\/p>\n

Additional characteristics: <\/strong><\/p>\n

Species control: <\/strong>rRNA sequencing<\/p>\n

Cryoconservation passage: <\/strong>8<\/p>\n

Area of application: <\/strong>biotechnology, fatty acid production, study of the Labyrinthulea life cycle<\/p>\n

 <\/p>\n

Source<\/strong><\/p>\n

Species: <\/strong>Thraustochytrium kinnei<\/em><\/p>\n

Tissue:<\/strong><\/p>\n

Date:<\/strong> 13.02.2025<\/p>\n

 <\/p>\n

Cell culture<\/strong><\/p>\n

Morphology:<\/strong> \u201ccolonies\u201d of cells<\/p>\n

Cell culture method: <\/strong>monolayer<\/p>\n

Cell culture medium: <\/strong>a) 790 By+ (ATCC); b) FAND culture medium: 17 ASW, 5% FBS,<\/p>\n

5% DMEM (prepared from powder on 17\u2030 ASW), x0.05 NEAA, x1 PenStrep<\/p>\n

Cell culture conditions:<\/strong> room temperature<\/p>\n

Passage protocol: <\/strong>manual passage (scraping and resuspending) at the ratio 1:10 — 1:100<\/p>\n

Cryoconservation: <\/strong>90% FBS, 10% DMSO<\/p>\n

Cryoconservation cell concentration: <\/strong>1 mln cells \/ ml<\/p>\n

Cell viability after cryoconservation: <\/strong>70%<\/p>\n

Additional information:<\/strong><\/p>\n

\u00a0<\/strong><\/p>\n

References:<\/strong> http:\/\/dx.doi.org\/10.7717\/peerj.12737<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

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