Collective Center of ICG SB RAS «Collection of Pluripotent Human and Mammalian Cell cultures for Biological and Biomedical Research»
The collection contains embryonic stem and induced pluripotent stem cells of mice and other animals. There are also immortalized cell lines and protist cultures.
Mouse embryonic stem cells can be used to produce transgenic animals.
Unique cell lines of American mink pluripotent stem cells allow studying the early embryonic development of mustelids.
Services:
— distribution of cell cultures;
— training.
Research areas:
— derivation of cell lines for fundamental and applied research in the fields of developmental biology, cell biology and transgenesis, including embryonic stem and induced pluripotent stem cells of humans, mice and other laboratory animals, primary and with genetic modifications;
— systematic description of the generated cell lines;
— quality control of collection material using modern methods.
Manager: Aleksei Gavriilovich Menzorov, Ph.D.
Website: http://ckp.icgen.ru/cells/
E-mail: cellbank@bionet.nsc.ru
Contents
Cell line passport of DGES1-TubbEGFPpuro
Cell line passport of DGES1-TubbEGFP
Cell line passport of DGES1-TubbEGFPSV40puro
American mink pluripotent stem cells
Cell line passport of CHO-hCNTN6-HA
Cell line passport of CHO-hDLL1-HA
Cell line passport of CHO-hNOTCH1-FLAG
Cell line passport of CHO-hNOTCH2-FLAG
Mouse pluripotent stem cells
Cell line passport of DGES1
Catalogue number: MMES00001
Name: DGES1
Description: mouse (Mus musculus) embryonic stem cells derived from 129S2/SvPasCrl 3.5D blastocyst
Authors: Menzorov A.G., Matveeva N.M.
Contamination analysis: bacteria, fungi and mycoplasma not detected
Karyotype: 2n=40, XY, chromosome number variability 39-41, tetraploid cells 6%, modal chromosome number 40
Pluripotency: pluripotency is shown by embrioid body formation, teratoma formation in SCID mice and germ line transmission
Additional characteristics:
Species control: cytogenetic
Cryoconservation passage: 6
Area of application: transgenesis, developmental biology
Source
Species: Mus musculus
Tissue: blastocyst
Date: 01.01.2016
Cell culture
Morphology: mouse pluripotent stem cell colonies
Cell culture method: monolayer
Cell culture medium: DMEM (glucose 4.5 g/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U/ml
Cell culture conditions: 37°C, 5% CO2
Passage protocol: passage with trypsin-EDTA 0.05%, split 1:3 — 1:6
Cryoconservation: 90% KSR, 10% DMSO
Cryoconservation cell concentration: 0.5 mln cells / ml
Cell viability after cryoconservation: 70%
Additional information: Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.
References: https://doi.org/10.1002/jcb.28981
Cell line passport of DGES2
Catalogue number: MMES00002
Name: DGES2
Description: mouse (Mus musculus) embryonic stem cells derived from 129S2/SvPasCrl 3.5D blastocyst
Authors: Menzorov A.G., Matveeva N.M.
Contamination analysis: bacteria, fungi and mycoplasma not detected
Karyotype: 2n=40, XY, chromosome number variability 39-41, tetraploid cells 5%, modal chromosome number 40
Pluripotency: pluripotency is shown by embrioid body formation, teratoma formation in SCID mice and germ line transmission
Additional characteristics:
Species control: cytogenetic
Cryoconservation passage: 6
Area of application: transgenesis, developmental biology
Source
Species: Mus musculus
Tissue: blastocyst
Date: 01.01.2016
Cell culture
Morphology: mouse pluripotent stem cell colonies
Cell culture method: monolayer
Cell culture medium: DMEM (glucose 4.5 g/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U/ml
Cell culture conditions: 37°C, 5% CO2
Passage protocol: passage with trypsin-EDTA 0.05%, split 1:3 — 1:6
Cryoconservation: 90% KSR, 10% DMSO
Cryoconservation cell concentration: 0.5 mln cells / ml
Cell viability after cryoconservation: 70%
Additional information: Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.
References: https://doi.org/10.1002/jcb.28981
Cell line passport of DGES1-TubbEGFPpuro
Catalogue number: MMES00038
Name: DGES1-TubbEGFPpuro
Description: DGES1 mouse embryonic stem cells with site-specific EGFP insertion after bTubb3 last exon via 2A peptide and puromycin resistance
Authors: Menzorov A.G.
Contamination analysis: bacteria, fungi and mycoplasma not detected
Karyotype: 2n=40, XY, chromosome number variability 39-41, tetraploid cells less than 10%, modal chromosome number 40
Pluripotency: pluripotency is shown by expression of specific markers
Additional characteristics:
Species control: cytogenetic
Cryoconservation passage: 24
Area of application: transgenesis, developmental biology
Source
Species: Mus musculus
Tissue:
Date: 01.01.2017
Cell culture
Morphology: mouse pluripotent stem cell colonies
Cell culture method: monolayer
Cell culture medium: DMEM (glucose 4.5 g/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U/ml
Cell culture conditions: 37°C, 5% CO2
Passage protocol: passage with trypsin-EDTA 0.05%, split 1:3 — 1:6
Cryoconservation: 90% KSR, 10% DMSO
Cryoconservation cell concentration: 0.5 mln cells / ml
Cell viability after cryoconservation: 70%
Additional information: Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.
References: https://doi.org/10.1002/jcb.28981
Cell line passport of DGES1-TubbEGFP
Catalogue number: MMES00039
Name: DGES1-TubbEGFP
Description: DGES1 mouse embryonic stem cells with site-specific EGFP insertion after bTubb3 last exon via 2A peptide
Authors: Menzorov A.G.
Contamination analysis: bacteria, fungi and mycoplasma not detected
Karyotype: 2n=40, XY, chromosome number variability 39-41, tetraploid cells less than 10%, modal chromosome number 40
Pluripotency: pluripotency is shown by expression of specific markers
Additional characteristics:
Species control: cytogenetic
Cryoconservation passage: 22
Area of application: transgenesis, developmental biology
Source
Species: Mus musculus
Tissue:
Date: 01.01.2017
Cell culture
Morphology: mouse pluripotent stem cell colonies
Cell culture method: monolayer
Cell culture medium: DMEM (glucose 4.5 g/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U/ml
Cell culture conditions: 37°C, 5% CO2
Passage protocol: passage with trypsin-EDTA 0.05%, split 1:3 — 1:6
Cryoconservation: 90% KSR, 10% DMSO
Cryoconservation cell concentration: 0.5 mln cells / ml
Cell viability after cryoconservation: 70%
Additional information: Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.
References: https://doi.org/10.1002/jcb.28981
Cell line passport of DGES1-TubbEGFPSV40puro
Catalogue number: MMES00040
Name: DGES1-TubbEGFPSV40puro
Description: DGES1 mouse embryonic stem cells with site-specific EGFP insertion after bTubb3 last exon via 2A peptide, SV40 polyA signal and puromycin resistance (site-specific and unspecific insert)
Authors: Menzorov A.G.
Contamination analysis: bacteria, fungi and mycoplasma not detected
Karyotype: 2n=40, XY, chromosome number variability 39-41, tetraploid cells less than 5%, modal chromosome number 40
Pluripotency: pluripotency is shown by expression of specific markers
Additional characteristics:
Species control: cytogenetic
Cryoconservation passage: 20
Area of application: transgenesis, developmental biology
Source
Species: Mus musculus
Tissue:
Date: 01.01.2017
Cell culture
Morphology: mouse pluripotent stem cell colonies
Cell culture method: monolayer
Cell culture medium: DMEM (glucose 4.5 g/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U/ml
Cell culture conditions: 37°C, 5% CO2
Passage protocol: passage with trypsin-EDTA 0.05%, split 1:3 — 1:6
Cryoconservation: 90% KSR, 10% DMSO
Cryoconservation cell concentration: 0.5 mln cells / ml
Cell viability after cryoconservation: 70%
Additional information: Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.
References: https://doi.org/10.1002/jcb.28981
Cell line passport of DGES1-TubbEGFPSV40
Catalogue number: MMES00041
Name: DGES1-TubbEGFPSV40
Description: DGES1 mouse embryonic stem cells with site-specific EGFP insertion after bTubb3 last exon via 2A peptide, SV40 polyA signal and puromycin resistance (unspecific insert)
Authors: Menzorov A.G.
Contamination analysis: bacteria, fungi and mycoplasma not detected
Karyotype: 2n=40, XY, chromosome number variability 39-40, tetraploid cells less than 2%, modal chromosome number 40
Pluripotency: pluripotency is shown by expression of specific markers
Additional characteristics:
Species control: cytogenetic
Cryoconservation passage: 26
Area of application: transgenesis, developmental biology
Source
Species: Mus musculus
Tissue:
Date: 01.01.2017
Cell culture
Morphology: mouse pluripotent stem cell colonies
Cell culture method: monolayer
Cell culture medium: DMEM (glucose 4.5 g/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U/ml
Cell culture conditions: 37°C, 5% CO2
Passage protocol: passage with trypsin-EDTA 0.05%, split 1:3 — 1:6
Cryoconservation: 90% KSR, 10% DMSO
Cryoconservation cell concentration: 0.5 mln cells / ml
Cell viability after cryoconservation: 70%
Additional information: Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.
References: https://doi.org/10.1002/jcb.28981
Cell line passport of MA01
Catalogue number: MMES00004
Name: MA01
Description: mouse (Mus musculus) embryonic stem cells derived from outbred BALB x 129/Ola blastocyst
Authors: Menzorov A.G.
Contamination analysis: bacteria, fungi and mycoplasma not detected
Karyotype: 2n=40, XY, chromosome number variability 39-45, tetraploid cells less than 2%, modal chromosome number 40
Pluripotency: pluripotency is shown by teratoma formation in NU/NU mice and germ line transmission
Additional characteristics:
Species control: cytogenetic
Cryoconservation passage: 4
Area of application: transgenesis, developmental biology
Source
Species: Mus musculus
Tissue: blastocyst
Date: 01.01.2016
Cell culture
Morphology: mouse pluripotent stem cell colonies
Cell culture method: monolayer
Cell culture medium: DMEM (glucose 4.5 g/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U/ml
Cell culture conditions: 37°C, 5% CO2
Passage protocol: passage with trypsin-EDTA 0.05%, split 1:3 — 1:6
Cryoconservation: 90% KSR, 10% DMSO
Cryoconservation cell concentration: 0.5 mln cells / ml
Cell viability after cryoconservation: 70%
Additional information: Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.
References: https://doi.org/10.1007/s10616-014-9751-y
Cell line passport of MA01-3E
Catalogue number: MMES00037
Name: MA01-3E
Description: MA01 mouse embryonic stem cells with EGFP cassette in Trim71 gene
Authors: Menzorov A.G.
Contamination analysis: bacteria, fungi and mycoplasma not detected
Karyotype: 2n=40, XY, chromosome number variability 39-41, tetraploid cells less than 2%, modal chromosome number 40
Pluripotency: pluripotency is shown by teratoma formation in SCID mice and germ line transmission
Additional characteristics:
Species control: cytogenetic
Cryoconservation passage: 15
Area of application: transgenesis, developmental biology
Source
Species: Mus musculus
Tissue:
Date: 01.01.2017
Cell culture
Morphology: mouse pluripotent stem cell colonies
Cell culture method: monolayer
Cell culture medium: DMEM (glucose 4.5 g/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U/ml
Cell culture conditions: 37°C, 5% CO2
Passage protocol: passage with trypsin-EDTA 0.05%, split 1:3 — 1:6
Cryoconservation: 90% KSR, 10% DMSO
Cryoconservation cell concentration: 0.5 mln cells / ml
Cell viability after cryoconservation: 70%
Additional information: Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.
References: https://doi.org/10.1007/s10616-014-9751-y
Cell line passport of MA02
Catalogue number: MMES00005
Name: MA02
Description: mouse (Mus musculus) embryonic stem cells derived from outbred BALB x 129/Ola blastocyst
Authors: Menzorov A.G.
Contamination analysis: bacteria, fungi and mycoplasma not detected
Karyotype: 2n=40, XX, chromosome number variability 39-41, tetraploid cells 13%, modal chromosome number 40
Pluripotency: pluripotency is shown by teratoma formation in NU/NU mice and chimeric animal generation
Additional characteristics:
Species control: cytogenetic
Cryoconservation passage: 4
Area of application: transgenesis, developmental biology
Source
Species: Mus musculus
Tissue: blastocyst
Date: 01.01.2016
Cell culture
Morphology: mouse pluripotent stem cell colonies
Cell culture method: monolayer
Cell culture medium: DMEM (glucose 4.5 g/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U/ml
Cell culture conditions: 37°C, 5% CO2
Passage protocol: passage with trypsin-EDTA 0.05%, split 1:3 — 1:6
Cryoconservation: 90% KSR, 10% DMSO
Cryoconservation cell concentration: 0.5 mln cells / ml
Cell viability after cryoconservation: 70%
Additional information: Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.
References: https://doi.org/10.1007/s10616-014-9751-y
Cell line passport of MA03
Catalogue number: MMES00006
Name: MA03
Description: mouse (Mus musculus) embryonic stem cells derived from outbred BALB x 129/Ola blastocyst
Authors: Menzorov A.G.
Contamination analysis: bacteria, fungi and mycoplasma not detected
Karyotype: 2n=40, XX, chromosome number variability 40-42, 71-83, tetraploid cells 79%, modal chromosome number 78, 79
Pluripotency: pluripotency is shown by expression of specific markers
Additional characteristics:
Species control: cytogenetic
Cryoconservation passage: 7
Area of application: transgenesis, developmental biology
Source
Species: Mus musculus
Tissue: blastocyst
Date: 01.01.2016
Cell culture
Morphology: mouse pluripotent stem cell colonies
Cell culture method: monolayer
Cell culture medium: DMEM (glucose 4.5 g/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U/ml
Cell culture conditions: 37°C, 5% CO2
Passage protocol: passage with trypsin-EDTA 0.05%, split 1:3 — 1:6
Cryoconservation: 90% KSR, 10% DMSO
Cryoconservation cell concentration: 0.5 mln cells / ml
Cell viability after cryoconservation: 70%
Additional information: Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.
References: https://doi.org/10.1007/s10616-014-9751-y
Cell line passport of MA04
Catalogue number: MMES00007
Name: MA04
Description: mouse (Mus musculus) embryonic stem cells derived from outbred BALB x 129/Ola blastocyst
Authors: Menzorov A.G.
Contamination analysis: bacteria, fungi and mycoplasma not detected
Karyotype: 2n=40, XX, chromosome number variability 39-42, tetraploid cells 28%, modal chromosome number 40
Pluripotency: pluripotency is shown by teratoma formation in NU/NU mice
Additional characteristics:
Species control: cytogenetic
Cryoconservation passage: 4
Area of application: transgenesis, developmental biology
Source
Species: Mus musculus
Tissue: blastocyst
Date: 01.01.2016
Cell culture
Morphology: mouse pluripotent stem cell colonies
Cell culture method: monolayer
Cell culture medium: DMEM (glucose 4.5 g/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U/ml
Cell culture conditions: 37°C, 5% CO2
Passage protocol: passage with trypsin-EDTA 0.05%, split 1:3 — 1:6
Cryoconservation: 90% KSR, 10% DMSO
Cryoconservation cell concentration: 0.5 mln cells / ml
Cell viability after cryoconservation: 70%
Additional information: Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.
References: https://doi.org/10.1007/s10616-014-9751-y
Cell line passport of MA05
Catalogue number: MMES00008
Name: MA05
Description: mouse (Mus musculus) embryonic stem cells derived from outbred BALB x 129/Ola blastocyst
Authors: Menzorov A.G.
Contamination analysis: bacteria, fungi and mycoplasma not detected
Karyotype: 2n=40, XY, chromosome number variability 39-42, tetraploid cells 18%, modal chromosome number 40
Pluripotency: pluripotency is shown by expression of specific markers
Additional characteristics:
Species control: cytogenetic
Cryoconservation passage: 8
Area of application: transgenesis, developmental biology
Source
Species: Mus musculus
Tissue: blastocyst
Date: 01.01.2016
Cell culture
Morphology: mouse pluripotent stem cell colonies
Cell culture method: monolayer
Cell culture medium: DMEM (glucose 4.5 g/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U/ml
Cell culture conditions: 37°C, 5% CO2
Passage protocol: passage with trypsin-EDTA 0.05%, split 1:3 — 1:6
Cryoconservation: 90% KSR, 10% DMSO
Cryoconservation cell concentration: 0.5 mln cells / ml
Cell viability after cryoconservation: 70%
Additional information: Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.
References: https://doi.org/10.1007/s10616-014-9751-y
Cell line passport of MA06
Catalogue number: MMES00009
Name: MA06
Description: mouse (Mus musculus) embryonic stem cells derived from outbred BALB x 129/Ola blastocyst
Authors: Menzorov A.G.
Contamination analysis: bacteria, fungi and mycoplasma not detected
Karyotype: 2n=40, XX, chromosome number variability 40-41, tetraploid cells 17%, modal chromosome number 40
Pluripotency: pluripotency is shown by teratoma formation in NU/NU mice and chimeric animal generation
Additional characteristics:
Species control: cytogenetic
Cryoconservation passage: 4
Area of application: transgenesis, developmental biology
Source
Species: Mus musculus
Tissue: blastocyst
Date: 01.01.2016
Cell culture
Morphology: mouse pluripotent stem cell colonies
Cell culture method: monolayer
Cell culture medium: DMEM (glucose 4.5 g/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U/ml
Cell culture conditions: 37°C, 5% CO2
Passage protocol: passage with trypsin-EDTA 0.05%, split 1:3 — 1:6
Cryoconservation: 90% KSR, 10% DMSO
Cryoconservation cell concentration: 0.5 mln cells / ml
Cell viability after cryoconservation: 70%
Additional information: Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.
References: https://doi.org/10.1007/s10616-014-9751-y
Cell line passport of MA07
Catalogue number: MMES00010
Name: MA07
Description: mouse (Mus musculus) embryonic stem cells derived from outbred BALB x 129/Ola blastocyst
Authors: Menzorov A.G.
Contamination analysis: bacteria, fungi and mycoplasma not detected
Karyotype: 2n=40, XX, chromosome number variability 39-43, tetraploid cells 24%, modal chromosome number 40
Pluripotency: pluripotency is shown by expression of specific markers
Additional characteristics:
Species control: cytogenetic
Cryoconservation passage: 4
Area of application: transgenesis, developmental biology
Source
Species: Mus musculus
Tissue: blastocyst
Date: 01.01.2016
Cell culture
Morphology: mouse pluripotent stem cell colonies
Cell culture method: monolayer
Cell culture medium: DMEM (glucose 4.5 g/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U/ml
Cell culture conditions: 37°C, 5% CO2
Passage protocol: passage with trypsin-EDTA 0.05%, split 1:3 — 1:6
Cryoconservation: 90% KSR, 10% DMSO
Cryoconservation cell concentration: 0.5 mln cells / ml
Cell viability after cryoconservation: 70%
Additional information: Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.
References: https://doi.org/10.1007/s10616-014-9751-y
Cell line passport of MA08
Catalogue number: MMES00011
Name: MA08
Description: mouse (Mus musculus) embryonic stem cells derived from outbred BALB x 129/Ola blastocyst
Authors: Menzorov A.G.
Contamination analysis: bacteria, fungi and mycoplasma not detected
Karyotype: 2n=40, XX, chromosome number variability 39-41, tetraploid cells 11%, modal chromosome number 40
Pluripotency: pluripotency is shown by expression of specific markers
Additional characteristics:
Species control: cytogenetic
Cryoconservation passage: 4
Area of application: transgenesis, developmental biology
Source
Species: Mus musculus
Tissue: blastocyst
Date: 01.01.2016
Cell culture
Morphology: mouse pluripotent stem cell colonies
Cell culture method: monolayer
Cell culture medium: DMEM (glucose 4.5 g/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U/ml
Cell culture conditions: 37°C, 5% CO2
Passage protocol: passage with trypsin-EDTA 0.05%, split 1:3 — 1:6
Cryoconservation: 90% KSR, 10% DMSO
Cryoconservation cell concentration: 0.5 mln cells / ml
Cell viability after cryoconservation: 70%
Additional information: Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.
References: https://doi.org/10.1007/s10616-014-9751-y
Cell line passport of MA09
Catalogue number: MMES00012
Name: MA09
Description: mouse (Mus musculus) embryonic stem cells derived from outbred BALB x 129/Ola blastocyst
Authors: Menzorov A.G.
Contamination analysis: bacteria, fungi and mycoplasma not detected
Karyotype: 2n=40, XY, chromosome number variability 39-41, tetraploid cells 8%, modal chromosome number 40
Pluripotency: pluripotency is shown by expression of specific markers
Additional characteristics:
Species control: cytogenetic
Cryoconservation passage: 4
Area of application: transgenesis, developmental biology
Source
Species: Mus musculus
Tissue: blastocyst
Date: 01.01.2016
Cell culture
Morphology: mouse pluripotent stem cell colonies
Cell culture method: monolayer
Cell culture medium: DMEM (glucose 4.5 g/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U/ml
Cell culture conditions: 37°C, 5% CO2
Passage protocol: passage with trypsin-EDTA 0.05%, split 1:3 — 1:6
Cryoconservation: 90% KSR, 10% DMSO
Cryoconservation cell concentration: 0.5 mln cells / ml
Cell viability after cryoconservation: 70%
Additional information: Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.
References: https://doi.org/10.1007/s10616-014-9751-y
Cell line passport of MA10
Catalogue number: MMES00013
Name: MA10
Description: mouse (Mus musculus) embryonic stem cells derived from outbred BALB x 129/Ola blastocyst
Authors: Menzorov A.G.
Contamination analysis: bacteria, fungi and mycoplasma not detected
Karyotype: 2n=40, XX, chromosome number variability 39-43, tetraploid cells 7%, modal chromosome number 40
Pluripotency: pluripotency is shown by expression of specific markers
Additional characteristics:
Species control: cytogenetic
Cryoconservation passage: 4
Area of application: transgenesis, developmental biology
Source
Species: Mus musculus
Tissue: blastocyst
Date: 01.01.2016
Cell culture
Morphology: mouse pluripotent stem cell colonies
Cell culture method: monolayer
Cell culture medium: DMEM (glucose 4.5 g/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U/ml
Cell culture conditions: 37°C, 5% CO2
Passage protocol: passage with trypsin-EDTA 0.05%, split 1:3 — 1:6
Cryoconservation: 90% KSR, 10% DMSO
Cryoconservation cell concentration: 0.5 mln cells / ml
Cell viability after cryoconservation: 70%
Additional information: Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.
References: https://doi.org/10.1007/s10616-014-9751-y
Cell line passport of MA11
Catalogue number: MMES00014
Name: MA11
Description: mouse (Mus musculus) embryonic stem cells derived from outbred BALB x 129/Ola blastocyst
Authors: Menzorov A.G.
Contamination analysis: bacteria, fungi and mycoplasma not detected
Karyotype: 2n=40, XX, chromosome number variability 39-41, tetraploid cells 20%, modal chromosome number 40
Pluripotency: pluripotency is shown by expression of specific markers
Additional characteristics:
Species control: cytogenetic
Cryoconservation passage: 7
Area of application: transgenesis, developmental biology
Source
Species: Mus musculus
Tissue: blastocyst
Date: 01.01.2016
Cell culture
Morphology: mouse pluripotent stem cell colonies
Cell culture method: monolayer
Cell culture medium: DMEM (glucose 4.5 g/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U/ml
Cell culture conditions: 37°C, 5% CO2
Passage protocol: passage with trypsin-EDTA 0.05%, split 1:3 — 1:6
Cryoconservation: 90% KSR, 10% DMSO
Cryoconservation cell concentration: 0.5 mln cells / ml
Cell viability after cryoconservation: 70%
Additional information: Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.
References: https://doi.org/10.1007/s10616-014-9751-y
Cell line passport of MA12
Catalogue number: MMES00015
Name: MA12
Description: mouse (Mus musculus) embryonic stem cells derived from outbred BALB x 129/Ola blastocyst
Authors: Menzorov A.G.
Contamination analysis: bacteria, fungi and mycoplasma not detected
Karyotype: 2n=40, XY, chromosome number variability 39-42, tetraploid cells 2%, modal chromosome number 40, 41
Pluripotency: pluripotency is shown by expression of specific markers
Additional characteristics:
Species control: cytogenetic
Cryoconservation passage: 5
Area of application: transgenesis, developmental biology
Source
Species: Mus musculus
Tissue: blastocyst
Date: 01.01.2016
Cell culture
Morphology: mouse pluripotent stem cell colonies
Cell culture method: monolayer
Cell culture medium: DMEM (glucose 4.5 g/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U/ml
Cell culture conditions: 37°C, 5% CO2
Passage protocol: passage with trypsin-EDTA 0.05%, split 1:3 — 1:6
Cryoconservation: 90% KSR, 10% DMSO
Cryoconservation cell concentration: 0.5 mln cells / ml
Cell viability after cryoconservation: 70%
Additional information: Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.
References: https://doi.org/10.1007/s10616-014-9751-y
Cell line passport of MA13
Catalogue number: MMES00016
Name: MA13
Description: mouse (Mus musculus) embryonic stem cells derived from outbred BALB x 129/Ola blastocyst
Authors: Menzorov A.G.
Contamination analysis: bacteria, fungi and mycoplasma not detected
Karyotype: 2n=40, X0, chromosome number variability 39-43, tetraploid cells 8,6%, modal chromosome number 39
Pluripotency: pluripotency is shown by expression of specific markers
Additional characteristics:
Species control: cytogenetic
Cryoconservation passage: 4
Area of application: transgenesis, developmental biology
Source
Species: Mus musculus
Tissue: blastocyst
Date: 01.01.2016
Cell culture
Morphology: mouse pluripotent stem cell colonies
Cell culture method: monolayer
Cell culture medium: DMEM (glucose 4.5 g/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U/ml
Cell culture conditions: 37°C, 5% CO2
Passage protocol: passage with trypsin-EDTA 0.05%, split 1:3 — 1:6
Cryoconservation: 90% KSR, 10% DMSO
Cryoconservation cell concentration: 0.5 mln cells / ml
Cell viability after cryoconservation: 70%
Additional information: Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.
References: https://doi.org/10.1007/s10616-014-9751-y
Cell line passport of MA15
Catalogue number: MMES00017
Name: MA15
Description: mouse (Mus musculus) embryonic stem cells derived from outbred BALB x 129/Ola blastocyst
Authors: Menzorov A.G.
Contamination analysis: bacteria, fungi and mycoplasma not detected
Karyotype: 2n=40, XY, chromosome number variability 39-45, tetraploid cells 5%, modal chromosome number 40
Pluripotency: pluripotency is shown by teratoma formation in NU/NU mice
Additional characteristics:
Species control: cytogenetic
Cryoconservation passage: 4
Area of application: transgenesis, developmental biology
Source
Species: Mus musculus
Tissue: blastocyst
Date: 01.01.2016
Cell culture
Morphology: mouse pluripotent stem cell colonies
Cell culture method: monolayer
Cell culture medium: DMEM (glucose 4.5 g/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U/ml
Cell culture conditions: 37°C, 5% CO2
Passage protocol: passage with trypsin-EDTA 0.05%, split 1:3 — 1:6
Cryoconservation: 90% KSR, 10% DMSO
Cryoconservation cell concentration: 0.5 mln cells / ml
Cell viability after cryoconservation: 70%
Additional information: Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.
References: https://doi.org/10.1007/s10616-014-9751-y
Cell line passport of MC01
Catalogue number: MMES00048
Name: MC01
Description: mouse (Mus musculus) embryonic stem cells derived from C57BL x (outbred BALB x 129/Ola) blastocyst
Authors: Menzorov A.G.
Contamination analysis: bacteria, fungi and mycoplasma not detected
Karyotype: 2n=40, XX, chromosome number variability 39-45, tetraploid cells 32%, modal chromosome number 41, 40
Pluripotency: pluripotency is shown by expression of specific markers
Additional characteristics:
Species control: cytogenetic
Cryoconservation passage: 4
Area of application: transgenesis, developmental biology
Source
Species: Mus musculus
Tissue: blastocyst
Date: 01.01.2016
Cell culture
Morphology: mouse pluripotent stem cell colonies
Cell culture method: monolayer
Cell culture medium: DMEM (glucose 4.5 g/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U/ml
Cell culture conditions: 37°C, 5% CO2
Passage protocol: passage with trypsin-EDTA 0.05%, split 1:3 — 1:6
Cryoconservation: 90% KSR, 10% DMSO
Cryoconservation cell concentration: 0.5 mln cells / ml
Cell viability after cryoconservation: 70%
Additional information: Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.
References: https://doi.org/10.1007/s10616-014-9751-y
Cell line passport of MC02
Catalogue number: MMES00049
Name: MC02
Description: mouse (Mus musculus) embryonic stem cells derived from C57BL x (outbred BALB x 129/Ola) blastocyst
Authors: Menzorov A.G.
Contamination analysis: bacteria, fungi and mycoplasma not detected
Karyotype: 2n=40, XY, chromosome number variability 39-43, tetraploid cells 16%, modal chromosome number 41, 40
Pluripotency: pluripotency is shown by expression of specific markers
Additional characteristics:
Species control: cytogenetic
Cryoconservation passage: 5
Area of application: transgenesis, developmental biology
Source
Species: Mus musculus
Tissue: blastocyst
Date: 01.01.2016
Cell culture
Morphology: mouse pluripotent stem cell colonies
Cell culture method: monolayer
Cell culture medium: DMEM (glucose 4.5 g/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U/ml
Cell culture conditions: 37°C, 5% CO2
Passage protocol: passage with trypsin-EDTA 0.05%, split 1:3 — 1:6
Cryoconservation: 90% KSR, 10% DMSO
Cryoconservation cell concentration: 0.5 mln cells / ml
Cell viability after cryoconservation: 70%
Additional information: Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.
References: https://doi.org/10.1007/s10616-014-9751-y
Cell line passport of MC03
Catalogue number: MMES00050
Name: MC03
Description: mouse (Mus musculus) embryonic stem cells derived from C57BL x (outbred BALB x 129/Ola) blastocyst
Authors: Menzorov A.G.
Contamination analysis: bacteria, fungi and mycoplasma not detected
Karyotype: 2n=40, XY, chromosome number variability 39-45, tetraploid cells 6%, modal chromosome number 40
Pluripotency: pluripotency is shown by teratoma formation in NU/NU mice
Additional characteristics:
Species control: cytogenetic
Cryoconservation passage: 6
Area of application: transgenesis, developmental biology
Source
Species: Mus musculus
Tissue: blastocyst
Date: 01.01.2016
Cell culture
Morphology: mouse pluripotent stem cell colonies
Cell culture method: monolayer
Cell culture medium: DMEM (glucose 4.5 g/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U/ml
Cell culture conditions: 37°C, 5% CO2
Passage protocol: passage with trypsin-EDTA 0.25%, split 1:3 — 1:6
Cryoconservation: 90% KSR, 10% DMSO
Cryoconservation cell concentration: 0.5 mln cells / ml
Cell viability after cryoconservation: 70%
Additional information: Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.
References: https://doi.org/10.1007/s10616-014-9751-y
Cell line passport of MC04
Catalogue number: MMES00051
Name: MC04
Description: mouse (Mus musculus) embryonic stem cells derived from C57BL x (outbred BALB x 129/Ola) blastocyst
Authors: Menzorov A.G.
Contamination analysis: bacteria, fungi and mycoplasma not detected
Karyotype: 2n=40, XY, chromosome number variability 41-45, tetraploid cells 15%, modal chromosome number 42
Pluripotency: pluripotency is shown by expression of specific markers
Additional characteristics:
Species control: cytogenetic
Cryoconservation passage: 5
Area of application: transgenesis, developmental biology
Source
Species: Mus musculus
Tissue: blastocyst
Date: 01.01.2016
Cell culture
Morphology: mouse pluripotent stem cell colonies
Cell culture method: monolayer
Cell culture medium: DMEM (glucose 4.5 g/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U/ml
Cell culture conditions: 37°C, 5% CO2
Passage protocol: passage with trypsin-EDTA 0.05%, split 1:3 — 1:6
Cryoconservation: 90% KSR, 10% DMSO
Cryoconservation cell concentration: 0.5 mln cells / ml
Cell viability after cryoconservation: 70%
Additional information: Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.
References: https://doi.org/10.1007/s10616-014-9751-y
Cell line passport of MC05
Catalogue number: MMES00052
Name: MC05
Description: mouse (Mus musculus) embryonic stem cells derived from C57BL x (outbred BALB x 129/Ola) blastocyst
Authors: Menzorov A.G.
Contamination analysis: bacteria, fungi and mycoplasma not detected
Karyotype: 2n=40, XY, chromosome number variability 39-40, tetraploid cells 15%, modal chromosome number 40
Pluripotency: pluripotency is shown by expression of specific markers
Additional characteristics:
Species control: cytogenetic
Cryoconservation passage: 4
Area of application: transgenesis, developmental biology
Source
Species: Mus musculus
Tissue: blastocyst
Date: 01.01.2016
Cell culture
Morphology: mouse pluripotent stem cell colonies
Cell culture method: monolayer
Cell culture medium: DMEM (glucose 4.5 g/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U/ml
Cell culture conditions: 37°C, 5% CO2
Passage protocol: passage with trypsin-EDTA 0.05%, split 1:3 — 1:6
Cryoconservation: 90% KSR, 10% DMSO
Cryoconservation cell concentration: 0.5 mln cells / ml
Cell viability after cryoconservation: 70%
Additional information: Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.
References: https://doi.org/10.1007/s10616-014-9751-y
Cell line passport of MC06
Catalogue number: MMES00053
Name: MC06
Description: mouse (Mus musculus) embryonic stem cells derived from C57BL x (outbred BALB x 129/Ola) blastocyst
Authors: Menzorov A.G.
Contamination analysis: bacteria, fungi and mycoplasma not detected
Karyotype: 2n=40, XX, chromosome number variability 39-41, tetraploid cells 52%, modal chromosome number 40, 78, 79
Pluripotency: pluripotency is shown by expression of specific markers
Additional characteristics:
Species control: cytogenetic
Cryoconservation passage: 4
Area of application: transgenesis, developmental biology
Source
Species: Mus musculus
Tissue: blastocyst
Date: 01.01.2016
Cell culture
Morphology: mouse pluripotent stem cell colonies
Cell culture method: monolayer
Cell culture medium: DMEM (glucose 4.5 g/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U/ml
Cell culture conditions: 37°C, 5% CO2
Passage protocol: passage with trypsin-EDTA 0.05%, split 1:3 — 1:6
Cryoconservation: 90% KSR, 10% DMSO
Cryoconservation cell concentration: 0.5 mln cells / ml
Cell viability after cryoconservation: 70%
Additional information: Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.
References: https://doi.org/10.1007/s10616-014-9751-y
Cell line passport of MC07
Catalogue number: MMES00054
Name: MC07
Description: mouse (Mus musculus) embryonic stem cells derived from C57BL x (outbred BALB x 129/Ola) blastocyst
Authors: Menzorov A.G.
Contamination analysis: bacteria, fungi and mycoplasma not detected
Karyotype: 2n=40, XX, chromosome number variability 40-43, tetraploid cells 18%, modal chromosome number 40
Pluripotency: pluripotency is shown by expression of specific markers
Additional characteristics:
Species control: cytogenetic
Cryoconservation passage: 4
Area of application: transgenesis, developmental biology
Source
Species: Mus musculus
Tissue: blastocyst
Date: 01.01.2016
Cell culture
Morphology: mouse pluripotent stem cell colonies
Cell culture method: monolayer
Cell culture medium: DMEM (glucose 4.5 g/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U/ml
Cell culture conditions: 37°C, 5% CO2
Passage protocol: passage with trypsin-EDTA 0.05%, split 1:3 — 1:6
Cryoconservation: 90% KSR, 10% DMSO
Cryoconservation cell concentration: 0.5 mln cells / ml
Cell viability after cryoconservation: 70%
Additional information: Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.
References: https://doi.org/10.1007/s10616-014-9751-y
Cell line passport of MC08
Catalogue number: MMES00055
Name: MC08
Description: mouse (Mus musculus) embryonic stem cells derived from C57BL x (outbred BALB x 129/Ola) blastocyst
Authors: Menzorov A.G.
Contamination analysis: bacteria, fungi and mycoplasma not detected
Karyotype: 2n=40, XY, chromosome number variability 39-41, tetraploid cells 21%, modal chromosome number 40
Pluripotency: pluripotency is shown by expression of specific markers
Additional characteristics:
Species control: cytogenetic
Cryoconservation passage: 10
Area of application: transgenesis, developmental biology
Source
Species: Mus musculus
Tissue: blastocyst
Date: 01.01.2016
Cell culture
Morphology: mouse pluripotent stem cell colonies
Cell culture method: monolayer
Cell culture medium: DMEM (glucose 4.5 g/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U/ml
Cell culture conditions: 37°C, 5% CO2
Passage protocol: passage with trypsin-EDTA 0.05%, split 1:3 — 1:6
Cryoconservation: 90% KSR, 10% DMSO
Cryoconservation cell concentration: 0.5 mln cells / ml
Cell viability after cryoconservation: 70%
Additional information: Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.
References: https://doi.org/10.1007/s10616-014-9751-y
Cell line passport of MC09
Catalogue number: MMES00056
Name: MC09
Description: mouse (Mus musculus) embryonic stem cells derived from C57BL x (outbred BALB x 129/Ola) blastocyst
Authors: Menzorov A.G.
Contamination analysis: bacteria, fungi and mycoplasma not detected
Karyotype: 2n=40, XX, chromosome number variability 40-43, tetraploid cells 18%, modal chromosome number 40
Pluripotency: pluripotency is shown by expression of specific markers
Additional characteristics:
Species control: cytogenetic
Cryoconservation passage: 4
Area of application: transgenesis, developmental biology
Source
Species: Mus musculus
Tissue: blastocyst
Date: 01.01.2016
Cell culture
Morphology: mouse pluripotent stem cell colonies
Cell culture method: monolayer
Cell culture medium: DMEM (glucose 4.5 g/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U/ml
Cell culture conditions: 37°C, 5% CO2
Passage protocol: passage with trypsin-EDTA 0.05%, split 1:3 — 1:6
Cryoconservation: 90% KSR, 10% DMSO
Cryoconservation cell concentration: 0.5 mln cells / ml
Cell viability after cryoconservation: 70%
Additional information: Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.
References: https://doi.org/10.1007/s10616-014-9751-y
Cell line passport of MC10
Catalogue number: MMES00057
Name: MC10
Description: mouse (Mus musculus) embryonic stem cells derived from C57BL x (outbred BALB x 129/Ola) blastocyst
Authors: Menzorov A.G.
Contamination analysis: bacteria, fungi and mycoplasma not detected
Karyotype: 2n=40, XY, chromosome number variability 40-43, tetraploid cells 18%, modal chromosome number 40
Pluripotency: pluripotency is shown by expression of specific markers
Additional characteristics:
Species control: cytogenetic
Cryoconservation passage: 11
Area of application: transgenesis, developmental biology
Source
Species: Mus musculus
Tissue: blastocyst
Date: 01.01.2016
Cell culture
Morphology: mouse pluripotent stem cell colonies
Cell culture method: monolayer
Cell culture medium: DMEM (glucose 4.5 g/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U/ml
Cell culture conditions: 37°C, 5% CO2
Passage protocol: passage with trypsin-EDTA 0.05%, split 1:3 — 1:6
Cryoconservation: 90% KSR, 10% DMSO
Cryoconservation cell concentration: 0.5 mln cells / ml
Cell viability after cryoconservation: 70%
Additional information: Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.
References: https://doi.org/10.1007/s10616-014-9751-y
Cell line passport of MC11
Catalogue number: MMES00058
Name: MC11
Description: mouse (Mus musculus) embryonic stem cells derived from C57BL x (outbred BALB x 129/Ola) blastocyst
Authors: Menzorov A.G.
Contamination analysis: bacteria, fungi and mycoplasma not detected
Karyotype: 2n=40, XX, chromosome number variability 40-43, tetraploid cells 25%, modal chromosome number 40
Pluripotency: pluripotency is shown by teratoma formation in NU/NU mice
Additional characteristics:
Species control: cytogenetic
Cryoconservation passage: 6
Area of application: transgenesis, developmental biology
Source
Species: Mus musculus
Tissue: blastocyst
Date: 01.01.2016
Cell culture
Morphology: mouse pluripotent stem cell colonies
Cell culture method: monolayer
Cell culture medium: DMEM (glucose 4.5 g/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U/ml
Cell culture conditions: 37°C, 5% CO2
Passage protocol: passage with trypsin-EDTA 0.05%, split 1:3 — 1:6
Cryoconservation: 90% KSR, 10% DMSO
Cryoconservation cell concentration: 0.5 mln cells / ml
Cell viability after cryoconservation: 70%
Additional information: Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.
References: https://doi.org/10.1007/s10616-014-9751-y
Cell line passport of MC12
Catalogue number: MMES00059
Name: MC12
Description: mouse (Mus musculus) embryonic stem cells derived from C57BL x (outbred BALB x 129/Ola) blastocyst
Authors: Menzorov A.G.
Contamination analysis: bacteria, fungi and mycoplasma not detected
Karyotype: 2n=40, XY, chromosome number variability 39-42, tetraploid cells 8%, modal chromosome number 40
Pluripotency: pluripotency is shown by teratoma formation in NU/NU mice
Additional characteristics:
Species control: cytogenetic
Cryoconservation passage: 3
Area of application: transgenesis, developmental biology
Source
Species: Mus musculus
Tissue: blastocyst
Date: 01.01.2016
Cell culture
Morphology: mouse pluripotent stem cell colonies
Cell culture method: monolayer
Cell culture medium: DMEM (glucose 4.5 g/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U/ml
Cell culture conditions: 37°C, 5% CO2
Passage protocol: passage with trypsin-EDTA 0.05%, split 1:3 — 1:6
Cryoconservation: 90% KSR, 10% DMSO
Cryoconservation cell concentration: 0.5 mln cells / ml
Cell viability after cryoconservation: 70%
Additional information: Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.
References: https://doi.org/10.1007/s10616-014-9751-y
Cell line passport of MC13
Catalogue number: MMES00060
Name: MC13
Description: mouse (Mus musculus) embryonic stem cells derived from C57BL x (outbred BALB x 129/Ola) blastocyst
Authors: Menzorov A.G.
Contamination analysis: bacteria, fungi and mycoplasma not detected
Karyotype: 2n=40, XX, chromosome number variability 39-43, tetraploid cells 8%, modal chromosome number 40
Pluripotency: pluripotency is shown by expression of specific markers
Additional characteristics:
Species control: cytogenetic
Cryoconservation passage: 8
Area of application: transgenesis, developmental biology
Source
Species: Mus musculus
Tissue: blastocyst
Date: 01.01.2016
Cell culture
Morphology: mouse pluripotent stem cell colonies
Cell culture method: monolayer
Cell culture medium: DMEM (glucose 4.5 g/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U/ml
Cell culture conditions: 37°C, 5% CO2
Passage protocol: passage with trypsin-EDTA 0.05%, split 1:3 — 1:6
Cryoconservation: 90% KSR, 10% DMSO
Cryoconservation cell concentration: 0.5 mln cells / ml
Cell viability after cryoconservation: 70%
Additional information: Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.
References: https://doi.org/10.1007/s10616-014-9751-y
Cell line passport of MC15
Catalogue number: MMES00061
Name: MC15
Description: mouse (Mus musculus) embryonic stem cells derived from C57BL x (outbred BALB x 129/Ola) blastocyst
Authors: Menzorov A.G.
Contamination analysis: bacteria, fungi and mycoplasma not detected
Karyotype: 2n=40, XY, chromosome number variability 40-44, tetraploid cells 20%, modal chromosome number 41, 42, 43
Pluripotency: pluripotency is shown by expression of specific markers
Additional characteristics:
Species control: cytogenetic
Cryoconservation passage: 3
Area of application: transgenesis, developmental biology
Source
Species: Mus musculus
Tissue: blastocyst
Date: 01.01.2016
Cell culture
Morphology: mouse pluripotent stem cell colonies
Cell culture method: monolayer
Cell culture medium: DMEM (glucose 4.5 g/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U/ml
Cell culture conditions: 37°C, 5% CO2
Passage protocol: passage with trypsin-EDTA 0.05%, split 1:3 — 1:6
Cryoconservation: 90% KSR, 10% DMSO
Cryoconservation cell concentration: 0.5 mln cells / ml
Cell viability after cryoconservation: 70%
Additional information: Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.
References: https://doi.org/10.1007/s10616-014-9751-y
Cell line passport of MD01
Catalogue number: MMES00062
Name: MD01
Description: mouse (Mus musculus) embryonic stem cells derived from DD/c x (outbred BALB x 129/Ola) blastocyst
Authors: Menzorov A.G.
Contamination analysis: bacteria, fungi and mycoplasma not detected
Karyotype: 2n=40, XX, chromosome number variability 39-44, tetraploid cells 26%, modal chromosome number 40
Pluripotency: pluripotency is shown by expression of specific markers
Additional characteristics:
Species control: cytogenetic
Cryoconservation passage: 7
Area of application: transgenesis, developmental biology
Source
Species: Mus musculus
Tissue: blastocyst
Date: 01.01.2016
Cell culture
Morphology: mouse pluripotent stem cell colonies
Cell culture method: monolayer
Cell culture medium: DMEM (glucose 4.5 g/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U/ml
Cell culture conditions: 37°C, 5% CO2
Passage protocol: passage with trypsin-EDTA 0.05%, split 1:3 — 1:6
Cryoconservation: 90% KSR, 10% DMSO
Cryoconservation cell concentration: 0.5 mln cells / ml
Cell viability after cryoconservation: 70%
Additional information: Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.
References: https://doi.org/10.1007/s10616-014-9751-y
Cell line passport of MD02
Catalogue number: MMES00063
Name: MD02
Description: mouse (Mus musculus) embryonic stem cells derived from DD/c x (outbred BALB x 129/Ola) blastocyst
Authors: Menzorov A.G.
Contamination analysis: bacteria, fungi and mycoplasma not detected
Karyotype: 2n=40, XXX0, chromosome number variability 40-48, 54-60, tetraploid cells 95%, modal chromosome number 80
Pluripotency: pluripotency is shown by expression of specific markers
Additional characteristics:
Species control: cytogenetic
Cryoconservation passage: 6
Area of application: transgenesis, developmental biology
Source
Species: Mus musculus
Tissue: blastocyst
Date: 01.01.2016
Cell culture
Morphology: mouse pluripotent stem cell colonies
Cell culture method: monolayer
Cell culture medium: DMEM (glucose 4.5 g/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U/ml
Cell culture conditions: 37°C, 5% CO2
Passage protocol: passage with trypsin-EDTA 0.05%, split 1:3 — 1:6
Cryoconservation: 90% KSR, 10% DMSO
Cryoconservation cell concentration: 0.5 mln cells / ml
Cell viability after cryoconservation: 70%
Additional information: Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.
References: https://doi.org/10.1007/s10616-014-9751-y
Hybrid cells
Cell line passport of tme13
Catalogue number: MMHC00042
Name: tme13
Description: hybrid cells produced by mouse tau-GFP ES cell and m5S embryonic fibroblast fusion, embryonic stem cell phenotype
Authors: Matveeva N.M.
Contamination analysis: bacteria, fungi and mycoplasma not detected
Karyotype: 4n=80, XX0, 83% of the cells have number of chromosomes 66-79
Pluripotency: pluripotency is shown by expression of specific markers
Additional characteristics:
Species control: cytogenetic
Cryoconservation passage: 10
Area of application: study of pluripotency, developmental biology
Source
Species: Mus musculus
Tissue:
Date: 01.01.2017
Cell culture
Morphology: mouse pluripotent stem cell colonies
Cell culture method: monolayer
Cell culture medium: DMEM (glucose 4.5 g/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U/ml
Cell culture conditions: 37°C, 5% CO2
Passage protocol: passage with trypsin-EDTA 0.05%, split 1:3 — 1:6
Cryoconservation: 90% KSR, 10% DMSO
Cryoconservation cell concentration: 0.5 mln cells / ml
Cell viability after cryoconservation: 70%
Additional information: Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.
References: https://doi.org/10.1038/s41598-017-18352-4
Cell line passport of tme14
Catalogue number: MMHC00043
Name: tme14
Description: hybrid cells produced by mouse tau-GFP ES cell and m5S embryonic fibroblast fusion, embryonic stem cell phenotype
Authors: Matveeva N.M.
Contamination analysis: bacteria, fungi and mycoplasma not detected
Karyotype: 4n=80, XX0, 92% of the cells have number of chromosomes 69-77
Pluripotency: pluripotency is shown by expression of specific markers
Additional characteristics:
Species control: cytogenetic
Cryoconservation passage: 17
Area of application: study of pluripotency, developmental biology
Source
Species: Mus musculus
Tissue:
Date: 01.01.2017
Cell culture
Morphology: mouse pluripotent stem cell colonies
Cell culture method: monolayer
Cell culture medium: DMEM (glucose 4.5 g/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U/ml
Cell culture conditions: 37°C, 5% CO2
Passage protocol: passage with trypsin-EDTA 0.05%, split 1:3 — 1:6
Cryoconservation: 90% KSR, 10% DMSO
Cryoconservation cell concentration: 0.5 mln cells / ml
Cell viability after cryoconservation: 70%
Additional information: Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.
References: https://doi.org/10.1038/s41598-017-18352-4
Cell line passport of tme17
Catalogue number: MMHC00044
Name: tme17
Description: hybrid cells produced by mouse tau-GFP ES cell and m5S embryonic fibroblast fusion, embryonic stem cell phenotype
Authors: Matveeva N.M.
Contamination analysis: bacteria, fungi and mycoplasma not detected
Karyotype: 4n=80, XX0, 75% of the cells have number of chromosomes 71-79
Pluripotency: pluripotency is shown by expression of specific markers
Additional characteristics:
Species control: cytogenetic
Cryoconservation passage: 10
Area of application: study of pluripotency, developmental biology
Source
Species: Mus musculus
Tissue:
Date: 01.01.2017
Cell culture
Morphology: mouse pluripotent stem cell colonies
Cell culture method: monolayer
Cell culture medium: DMEM (glucose 4.5 g/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U/ml
Cell culture conditions: 37°C, 5% CO2
Passage protocol: passage with trypsin-EDTA 0.05%, split 1:3 — 1:6
Cryoconservation: 90% KSR, 10% DMSO
Cryoconservation cell concentration: 0.5 mln cells / ml
Cell viability after cryoconservation: 70%
Additional information: Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.
References: https://doi.org/10.1038/s41598-017-18352-4
Cell line passport of tmf1
Catalogue number: MMHC00045
Name: tmf1
Description: hybrid cells produced by mouse tau-GFP ES cell and m5S embryonic fibroblast fusion, fibroblast phenotype
Authors: Matveeva N.M.
Contamination analysis: bacteria, fungi and mycoplasma not detected
Karyotype: 4n=80, XXY, 90% of the cells have number of chromosomes 69-82
Pluripotency:
Additional characteristics:
Species control: cytogenetic
Cryoconservation passage: 7
Area of application: study of pluripotency, developmental biology
Source
Species: Mus musculus
Tissue:
Date: 01.01.2017
Cell culture
Morphology: fibroblast morphology
Cell culture method: monolayer
Cell culture medium: DMEM (glucose 4.5 g/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U/ml
Cell culture conditions: 37°C, 5% CO2
Passage protocol: passage with trypsin-EDTA 0.25%, split 1:3 — 1:6
Cryoconservation: 90% FBS, 10% DMSO
Cryoconservation cell concentration: 0.5 mln cells / ml
Cell viability after cryoconservation: 70%
Additional information:
References: https://doi.org/10.1038/s41598-017-18352-4
Cell line passport of tmf2
Catalogue number: MMHC00046
Name: tmf2
Description: hybrid cells produced by mouse tau-GFP ES cell and m5S embryonic fibroblast fusion, fibroblast phenotype
Authors: Matveeva N.M.
Contamination analysis: bacteria, fungi and mycoplasma not detected
Karyotype: 4n=80, XXY, 76% of the cells have number of chromosomes 71-82
Pluripotency:
Additional characteristics:
Species control: cytogenetic
Cryoconservation passage: 6
Area of application: study of pluripotency, developmental biology
Source
Species: Mus musculus
Tissue:
Date: 01.01.2017
Cell culture
Morphology: fibroblast morphology
Cell culture method: monolayer
Cell culture medium: DMEM (glucose 4.5 g/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U/ml
Cell culture conditions: 37°C, 5% CO2
Passage protocol: passage with trypsin-EDTA 0.25%, split 1:3 — 1:6
Cryoconservation: 90% FBS, 10% DMSO
Cryoconservation cell concentration: 0.5 mln cells / ml
Cell viability after cryoconservation: 70%
Additional information:
References: https://doi.org/10.1038/s41598-017-18352-4
Cell line passport of tmf5
Catalogue number: MMHC00047
Name: tmf5
Description: hybrid cells produced by mouse tau-GFP ES cell and m5S embryonic fibroblast fusion, fibroblast phenotype
Authors: Matveeva N.M.
Contamination analysis: bacteria, fungi and mycoplasma not detected
Karyotype: 4n=80, XXY, 86% of the cells have number of chromosomes 73-81
Pluripotency:
Additional characteristics:
Species control: cytogenetic
Cryoconservation passage: 6
Area of application: study of pluripotency, developmental biology
Source
Species: Mus musculus
Tissue:
Date: 01.01.2017
Cell culture
Morphology: fibroblast morphology
Cell culture method: monolayer
Cell culture medium: DMEM (glucose 4.5 g/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U/ml
Cell culture conditions: 37°C, 5% CO2
Passage protocol: passage with trypsin-EDTA 0.25%, split 1:3 — 1:6
Cryoconservation: 90% FBS, 10% DMSO
Cryoconservation cell concentration: 0.5 mln cells / ml
Cell viability after cryoconservation: 70%
Additional information:
References: https://doi.org/10.1038/s41598-017-18352-4
American mink pluripotent stem cells
Cell line passport of MES12
Catalogue number: NVES00003
Name: MES12
Description: american mink (Neovison vison) embryonic stem cells derived from morula
Authors: Sukoyan M.A.
Contamination analysis: bacteria, fungi and mycoplasma not detected
Karyotype: 2n=30, XY
Pluripotency: pluripotency is shown by embrioid body formation and teratoma formation in immunodeficient mice
Additional characteristics:
Species control: cytogenetic
Cryoconservation passage: 11
Area of application: study of pluripotency, developmental biology
Source
Species: Neogale vison
Tissue: morula
Date: 01.01.1993
Cell culture
Morphology: mink pluripotent stem cell colonies
Cell culture method: monolayer
Cell culture medium: a-MEM, ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM
Cell culture conditions: 37°C, 5% CO2
Passage protocol: passage with trypsin-EDTA 0.25%, split 1:3 — 1:6
Cryoconservation: 90% KSR, 10% DMSO
Cryoconservation cell concentration: 0.5 mln cells / ml
Cell viability after cryoconservation: 70%
Additional information: Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.
References: https://doi.org/10.1186/1471-2164-16-S13-S6
Cell line passport of MES20
Catalogue number: NVES00018
Name: MES20
Description: american mink (Neovison vison) embryonic stem cells derived from morula
Authors: Matveeva N.M.
Contamination analysis: bacteria, fungi and mycoplasma not detected
Karyotype: 2n=30, XY, chromosome number variability 29-30, ~60, tetraploid cells 71%, modal chromosome number ~60
Pluripotency: pluripotency is shown byteratoma formation in SCID mice
Additional characteristics:
Species control: cytogenetic
Cryoconservation passage: 4
Area of application: study of pluripotency, developmental biology
Source
Species: Neogale vison
Tissue: morula
Date: 01.01.2015
Cell culture
Morphology: mink pluripotent stem cell colonies
Cell culture method: monolayer
Cell culture medium: DMEM (glucose 4.5 g/l), ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM, LIF 1000 U/ml
Cell culture conditions: 37°C, 5% CO2
Passage protocol: passage with trypsin-EDTA 0.05%, split 1:3 — 1:6
Cryoconservation: 90% KSR, 10% DMSO
Cryoconservation cell concentration: 0.5 mln cells / ml
Cell viability after cryoconservation: 70%
Additional information: Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.
References: https://doi.org/10.1186/1471-2164-16-S13-S6
Cell line passport of MES22
Catalogue number: NVES00019
Name: MES22
Description: american mink (Neovison vison) embryonic stem cells derived from morula
Authors: Matveeva N.M.
Contamination analysis: bacteria, fungi and mycoplasma not detected
Karyotype: 2n=30, XY, chromosome number variability 29-32, tetraploid cells 18%, modal chromosome number 30
Pluripotency: pluripotency is shown byteratoma formation in SCID mice
Additional characteristics:
Species control: cytogenetic
Cryoconservation passage: 10
Area of application: study of pluripotency, developmental biology
Source
Species: Neogale vison
Tissue: morula
Date: 01.01.2015
Cell culture
Morphology: mink pluripotent stem cell colonies
Cell culture method: monolayer
Cell culture medium: a-MEM, ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM
Cell culture conditions: 37°C, 5% CO2
Passage protocol: passage with trypsin-EDTA 0.25%, split 1:3 — 1:6
Cryoconservation: 90% KSR, 10% DMSO
Cryoconservation cell concentration: 0.5 mln cells / ml
Cell viability after cryoconservation: 70%
Additional information: Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.
References: https://doi.org/10.1186/1471-2164-16-S13-S6
Cell line passport of MES24
Catalogue number: NVES00020
Name: MES24
Description: american mink (Neovison vison) embryonic stem cells derived from morula
Authors: Matveeva N.M.
Contamination analysis: bacteria, fungi and mycoplasma not detected
Karyotype: 2n=30, XY, chromosome number variability 29-32, tetraploid cells 12%, modal chromosome number 30
Pluripotency: pluripotency is shown byteratoma formation in SCID mice
Additional characteristics:
Species control: cytogenetic
Cryoconservation passage: 3
Area of application: study of pluripotency, developmental biology
Source
Species: Neogale vison
Tissue: morula
Date: 01.01.2015
Cell culture
Morphology: mink pluripotent stem cell colonies
Cell culture method: monolayer
Cell culture medium: a-MEM, ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM
Cell culture conditions: 37°C, 5% CO2
Passage protocol: passage with trypsin-EDTA 0.25%, split 1:3 — 1:6
Cryoconservation: 90% KSR, 10% DMSO
Cryoconservation cell concentration: 0.5 mln cells / ml
Cell viability after cryoconservation: 70%
Additional information: Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.
References: https://doi.org/10.1186/1471-2164-16-S13-S6
Cell line passport of MES25
Catalogue number: NVES00021
Name: MES25
Description: american mink (Neovison vison) embryonic stem cells derived from morula
Authors: Matveeva N.M.
Contamination analysis: bacteria, fungi and mycoplasma not detected
Karyotype: 2n=30, XX, chromosome number variability 29-30, tetraploid cells 6%, modal chromosome number 30
Pluripotency: pluripotency is shown by expression of specific markers
Additional characteristics:
Species control: cytogenetic
Cryoconservation passage: 3
Area of application: study of pluripotency, developmental biology
Source
Species: Neogale vison
Tissue: morula
Date: 01.01.2015
Cell culture
Morphology: mink pluripotent stem cell colonies
Cell culture method: monolayer
Cell culture medium: a-MEM, ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM
Cell culture conditions: 37°C, 5% CO2
Passage protocol: passage with trypsin-EDTA 0.25%, split 1:3 — 1:6
Cryoconservation: 90% KSR, 10% DMSO
Cryoconservation cell concentration: 0.5 mln cells / ml
Cell viability after cryoconservation: 70%
Additional information: Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.
References: https://doi.org/10.1186/1471-2164-16-S13-S6
Cell line passport of MES27
Catalogue number: NVES00022
Name: MES27
Description: american mink (Neovison vison) embryonic stem cells derived from morula
Authors: Matveeva N.M.
Contamination analysis: bacteria, fungi and mycoplasma not detected
Karyotype: 2n=30, XY, chromosome number variability 29-32, tetraploid cells 7%, modal chromosome number 30
Pluripotency: pluripotency is shown by expression of specific markers
Additional characteristics:
Species control: cytogenetic
Cryoconservation passage: 4
Area of application: study of pluripotency, developmental biology
Source
Species: Neogale vison
Tissue: morula
Date: 01.01.2015
Cell culture
Morphology: mink pluripotent stem cell colonies
Cell culture method: monolayer
Cell culture medium: a-MEM, ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM
Cell culture conditions: 37°C, 5% CO2
Passage protocol: passage with trypsin-EDTA 0.25%, split 1:3 — 1:6
Cryoconservation: 90% KSR, 10% DMSO
Cryoconservation cell concentration: 0.5 mln cells / ml
Cell viability after cryoconservation: 70%
Additional information: Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.
References: https://doi.org/10.1186/1471-2164-16-S13-S6
Cell line passport of MES29
Catalogue number: NVES00023
Name: MES29
Description: american mink (Neovison vison) embryonic stem cells derived from morula
Authors: Matveeva N.M.
Contamination analysis: bacteria, fungi and mycoplasma not detected
Karyotype: 2n=30, XY, chromosome number variability 29-32, tetraploid cells 6%, modal chromosome number 30
Pluripotency: pluripotency is shown byteratoma formation in SCID mice
Additional characteristics:
Species control: cytogenetic
Cryoconservation passage: 3
Area of application: study of pluripotency, developmental biology
Source
Species: Neogale vison
Tissue: morula
Date: 01.01.2015
Cell culture
Morphology: mink pluripotent stem cell colonies
Cell culture method: monolayer
Cell culture medium: a-MEM, ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM
Cell culture conditions: 37°C, 5% CO2
Passage protocol: passage with trypsin-EDTA 0.25%, split 1:3 — 1:6
Cryoconservation: 90% KSR, 10% DMSO
Cryoconservation cell concentration: 0.5 mln cells / ml
Cell viability after cryoconservation: 70%
Additional information: Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.
References: https://doi.org/10.1186/1471-2164-16-S13-S6
Cell line passport of iNV1XX1
Catalogue number: NVPS00066
Name: iNV1XX1
Description: american mink (Neovison vison) induced pluripotent stem cells derived from embryonic fibroblasts
Authors: Menzorov A.G.
Contamination analysis: bacteria, fungi and mycoplasma not detected
Karyotype: 2n=30, XX, chromosome number variability 29-35, tetraploid cells 2%, modal chromosome number 30
Pluripotency: pluripotency is shown by expression of specific markers
Additional characteristics:
Species control: cytogenetic
Cryoconservation passage: 3
Area of application: study of pluripotency, developmental biology
Source
Species: Neogale vison
Tissue: fibroblasts
Date: 01.11.2017
Cell culture
Morphology: mink pluripotent stem cell colonies
Cell culture method: monolayer
Cell culture medium: a-MEM, ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM
Cell culture conditions: 37°C, 5% CO2
Passage protocol: passage with trypsin-EDTA 0.25%, split 1:3 — 1:6
Cryoconservation: 90% KSR, 10% DMSO
Cryoconservation cell concentration: 0.5 mln cells / ml
Cell viability after cryoconservation: 70%
Additional information: Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.
References: https://doi.org/10.18699/LettersVJ-2022-8-10
Cell line passport of iNV1XX2
Catalogue number: NVPS00067
Name: iNV1XX2
Description: american mink (Neovison vison) induced pluripotent stem cells derived from embryonic fibroblasts
Authors: Menzorov A.G.
Contamination analysis: bacteria, fungi and mycoplasma not detected
Karyotype: 2n=30, XX, chromosome number variability 29-35, tetraploid cells 4%, modal chromosome number 30
Pluripotency: pluripotency is shown by expression of specific markers
Additional characteristics:
Species control: cytogenetic
Cryoconservation passage: 3
Area of application: study of pluripotency, developmental biology
Source
Species: Neogale vison
Tissue: fibroblasts
Date: 01.11.2017
Cell culture
Morphology: mink pluripotent stem cell colonies
Cell culture method: monolayer
Cell culture medium: a-MEM, ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM
Cell culture conditions: 37°C, 5% CO2
Passage protocol: passage with trypsin-EDTA 0.25%, split 1:3 — 1:6
Cryoconservation: 90% KSR, 10% DMSO
Cryoconservation cell concentration: 0.5 mln cells / ml
Cell viability after cryoconservation: 70%
Additional information: Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.
References: https://doi.org/10.18699/LettersVJ-2022-8-10
Cell line passport of iNV3
Catalogue number: NVPS00024
Name: iNV3
Description: american mink (Neovison vison) induced pluripotent stem cells derived from embryonic fibroblasts
Authors: Menzorov A.G., Matveeva N.M., Khabarova A.A.
Contamination analysis: bacteria, fungi and mycoplasma not detected
Karyotype: 2n=30, XY, chromosome number variability 29-32, modal chromosome number 30
Pluripotency: pluripotency is shown byteratoma formation in SCID mice
Additional characteristics:
Species control: cytogenetic
Cryoconservation passage: 3
Area of application: study of pluripotency, developmental biology
Source
Species: Neogale vison
Tissue: fibroblasts
Date: 01.01.2015
Cell culture
Morphology: mink pluripotent stem cell colonies
Cell culture method: monolayer
Cell culture medium: a-MEM, ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM
Cell culture conditions: 37°C, 5% CO2
Passage protocol: passage with trypsin-EDTA 0.25%, split 1:3 — 1:6
Cryoconservation: 90% KSR, 10% DMSO
Cryoconservation cell concentration: 0.5 mln cells / ml
Cell viability after cryoconservation: 70%
Additional information: Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.
References: https://doi.org/10.1186/1471-2164-16-S13-S6
Cell line passport of iNV5
Catalogue number: NVPS00025
Name: iNV5
Description: american mink (Neovison vison) induced pluripotent stem cells derived from embryonic fibroblasts
Authors: Menzorov A.G., Matveeva N.M., Khabarova A.A.
Contamination analysis: bacteria, fungi and mycoplasma not detected
Karyotype: 2n=30, XY, chromosome number variability 29-30, tetraploid cells 7%, modal chromosome number 30
Pluripotency: pluripotency is shown byteratoma formation in SCID mice
Additional characteristics:
Species control: cytogenetic
Cryoconservation passage: 3
Area of application: study of pluripotency, developmental biology
Source
Species: Neogale vison
Tissue: fibroblasts
Date: 01.01.2015
Cell culture
Morphology: mink pluripotent stem cell colonies
Cell culture method: monolayer
Cell culture medium: a-MEM, ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM
Cell culture conditions: 37°C, 5% CO2
Passage protocol: passage with trypsin-EDTA 0.25%, split 1:3 — 1:6
Cryoconservation: 90% KSR, 10% DMSO
Cryoconservation cell concentration: 0.5 mln cells / ml
Cell viability after cryoconservation: 70%
Additional information: Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.
References: https://doi.org/10.1186/1471-2164-16-S13-S6
Cell line passport of iNV6
Catalogue number: NVPS00026
Name: iNV6
Description: american mink (Neovison vison) induced pluripotent stem cells derived from embryonic fibroblasts
Authors: Menzorov A.G., Matveeva N.M., Khabarova A.A.
Contamination analysis: bacteria, fungi and mycoplasma not detected
Karyotype: 2n=30, XY, chromosome number variability 29-32, tetraploid cells 7%, modal chromosome number 30
Pluripotency: pluripotency is shown byteratoma formation in SCID mice
Additional characteristics:
Species control: cytogenetic
Cryoconservation passage: 6
Area of application: study of pluripotency, developmental biology
Source
Species: Neogale vison
Tissue: fibroblasts
Date: 01.01.2015
Cell culture
Morphology: mink pluripotent stem cell colonies
Cell culture method: monolayer
Cell culture medium: a-MEM, ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM
Cell culture conditions: 37°C, 5% CO2
Passage protocol: passage with trypsin-EDTA 0.25%, split 1:3 — 1:6
Cryoconservation: 90% KSR, 10% DMSO
Cryoconservation cell concentration: 0.5 mln cells / ml
Cell viability after cryoconservation: 70%
Additional information: Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.
References: https://doi.org/10.1186/1471-2164-16-S13-S6
Cell line passport of iNV7
Catalogue number: NVPS00027
Name: iNV7
Description: american mink (Neovison vison) induced pluripotent stem cells derived from embryonic fibroblasts
Authors: Menzorov A.G., Matveeva N.M., Khabarova A.A.
Contamination analysis: bacteria, fungi and mycoplasma not detected
Karyotype: 2n=30, XY, chromosome number variability 30-32, tetraploid cells 10%, modal chromosome number 30
Pluripotency: pluripotency is shown byteratoma formation in SCID mice
Additional characteristics:
Species control: cytogenetic
Cryoconservation passage: 10
Area of application: study of pluripotency, developmental biology
Source
Species: Neogale vison
Tissue: fibroblasts
Date: 01.01.2015
Cell culture
Morphology: mink pluripotent stem cell colonies
Cell culture method: monolayer
Cell culture medium: a-MEM, ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM
Cell culture conditions: 37°C, 5% CO2
Passage protocol: passage with trypsin-EDTA 0.25%, split 1:3 — 1:6
Cryoconservation: 90% KSR, 10% DMSO
Cryoconservation cell concentration: 0.5 mln cells / ml
Cell viability after cryoconservation: 70%
Additional information: Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.
References: https://doi.org/10.1186/1471-2164-16-S13-S6
Cell line passport of iNV9
Catalogue number: NVPS00028
Name: iNV9
Description: american mink (Neovison vison) induced pluripotent stem cells derived from embryonic fibroblasts
Authors: Menzorov A.G., Matveeva N.M., Khabarova A.A.
Contamination analysis: bacteria, fungi and mycoplasma not detected
Karyotype: 2n=30, XY, chromosome number variability 29-30, tetraploid cells 9%, modal chromosome number 30
Pluripotency: pluripotency is shown by expression of specific markers
Additional characteristics:
Species control: cytogenetic
Cryoconservation passage: 12
Area of application: study of pluripotency, developmental biology
Source
Species: Neogale vison
Tissue: fibroblasts
Date: 01.01.2015
Cell culture
Morphology: mink pluripotent stem cell colonies
Cell culture method: monolayer
Cell culture medium: a-MEM, ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM
Cell culture conditions: 37°C, 5% CO2
Passage protocol: passage with trypsin-EDTA 0.25%, split 1:3 — 1:6
Cryoconservation: 90% KSR, 10% DMSO
Cryoconservation cell concentration: 0.5 mln cells / ml
Cell viability after cryoconservation: 70%
Additional information: Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.
References: https://doi.org/10.1186/1471-2164-16-S13-S6
Cell line passport of iNV11
Catalogue number: NVPS00029
Name: iNV11
Description: american mink (Neovison vison) induced pluripotent stem cells derived from embryonic fibroblasts
Authors: Menzorov A.G., Matveeva N.M., Khabarova A.A.
Contamination analysis: bacteria, fungi and mycoplasma not detected
Karyotype: 2n=30, XY, chromosome number variability 29-32, tetraploid cells 9%, modal chromosome number 30
Pluripotency: pluripotency is shown byteratoma formation in SCID mice
Additional characteristics:
Species control: cytogenetic
Cryoconservation passage: 11
Area of application: study of pluripotency, developmental biology
Source
Species: Neogale vison
Tissue: fibroblasts
Date: 01.01.2015
Cell culture
Morphology: mink pluripotent stem cell colonies
Cell culture method: monolayer
Cell culture medium: a-MEM, ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM
Cell culture conditions: 37°C, 5% CO2
Passage protocol: passage with trypsin-EDTA 0.25%, split 1:3 — 1:6
Cryoconservation: 90% KSR, 10% DMSO
Cryoconservation cell concentration: 0.5 mln cells / ml
Cell viability after cryoconservation: 70%
Additional information: Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.
References: https://doi.org/10.1186/1471-2164-16-S13-S6
Cell line passport of iNV13
Catalogue number: NVPS00030
Name: iNV13
Description: american mink (Neovison vison) induced pluripotent stem cells derived from embryonic fibroblasts
Authors: Menzorov A.G., Matveeva N.M., Khabarova A.A.
Contamination analysis: bacteria, fungi and mycoplasma not detected
Karyotype: 2n=30, XY, chromosome number variability 29-30, tetraploid cells 3%, modal chromosome number 30
Pluripotency: pluripotency is shown byteratoma formation in SCID mice
Additional characteristics:
Species control: cytogenetic
Cryoconservation passage: 3
Area of application: study of pluripotency, developmental biology
Source
Species: Neogale vison
Tissue: fibroblasts
Date: 01.01.2015
Cell culture
Morphology: mink pluripotent stem cell colonies
Cell culture method: monolayer
Cell culture medium: a-MEM, ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM
Cell culture conditions: 37°C, 5% CO2
Passage protocol: passage with trypsin-EDTA 0.25%, split 1:3 — 1:6
Cryoconservation: 90% KSR, 10% DMSO
Cryoconservation cell concentration: 0.5 mln cells / ml
Cell viability after cryoconservation: 70%
Additional information: Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.
References: https://doi.org/10.1186/1471-2164-16-S13-S6
Cell line passport of iNV15
Catalogue number: NVPS00031
Name: iNV15
Description: american mink (Neovison vison) induced pluripotent stem cells derived from embryonic fibroblasts
Authors: Menzorov A.G., Matveeva N.M., Khabarova A.A.
Contamination analysis: bacteria, fungi and mycoplasma not detected
Karyotype: 2n=30, XY, chromosome number variability 29-32, tetraploid cells 6%, modal chromosome number 30
Pluripotency: pluripotency is shown byteratoma formation in SCID mice
Additional characteristics:
Species control: cytogenetic
Cryoconservation passage: 3
Area of application: study of pluripotency, developmental biology
Source
Species: Neogale vison
Tissue: fibroblasts
Date: 01.01.2015
Cell culture
Morphology: mink pluripotent stem cell colonies
Cell culture method: monolayer
Cell culture medium: a-MEM, ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM
Cell culture conditions: 37°C, 5% CO2
Passage protocol: passage with trypsin-EDTA 0.25%, split 1:3 — 1:6
Cryoconservation: 90% KSR, 10% DMSO
Cryoconservation cell concentration: 0.5 mln cells / ml
Cell viability after cryoconservation: 70%
Additional information: Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.
References: https://doi.org/10.1186/1471-2164-16-S13-S6
Cell line passport of iNV18
Catalogue number: NVPS00032
Name: iNV18
Description: american mink (Neovison vison) induced pluripotent stem cells derived from embryonic fibroblasts
Authors: Menzorov A.G., Matveeva N.M., Khabarova A.A.
Contamination analysis: bacteria, fungi and mycoplasma not detected
Karyotype: 2n=30, XY, chromosome number variability 29-32, tetraploid cells 8%, modal chromosome number 30
Pluripotency: pluripotency is shown by expression of specific markers
Additional characteristics:
Species control: cytogenetic
Cryoconservation passage: 6
Area of application: study of pluripotency, developmental biology
Source
Species: Neogale vison
Tissue: fibroblasts
Date: 01.01.2015
Cell culture
Morphology: mink pluripotent stem cell colonies
Cell culture method: monolayer
Cell culture medium: a-MEM, ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM
Cell culture conditions: 37°C, 5% CO2
Passage protocol: passage with trypsin-EDTA 0.25%, split 1:3 — 1:6
Cryoconservation: 90% KSR, 10% DMSO
Cryoconservation cell concentration: 0.5 mln cells / ml
Cell viability after cryoconservation: 70%
Additional information: Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.
References: https://doi.org/10.1186/1471-2164-16-S13-S6
Cell line passport of iNV19
Catalogue number: NVPS00033
Name: iNV19
Description: american mink (Neovison vison) induced pluripotent stem cells derived from embryonic fibroblasts
Authors: Menzorov A.G., Matveeva N.M., Khabarova A.A.
Contamination analysis: bacteria, fungi and mycoplasma not detected
Karyotype: 2n=30, XY, chromosome number variability 29-30, tetraploid cells 8%, modal chromosome number 30
Pluripotency: pluripotency is shown byteratoma formation in SCID mice
Additional characteristics:
Species control: cytogenetic
Cryoconservation passage: 4
Area of application: study of pluripotency, developmental biology
Source
Species: Neogale vison
Tissue: fibroblasts
Date: 01.01.2015
Cell culture
Morphology: mink pluripotent stem cell colonies
Cell culture method: monolayer
Cell culture medium: a-MEM, ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM
Cell culture conditions: 37°C, 5% CO2
Passage protocol: passage with trypsin-EDTA 0.25%, split 1:3 — 1:6
Cryoconservation: 90% KSR, 10% DMSO
Cryoconservation cell concentration: 0.5 mln cells / ml
Cell viability after cryoconservation: 70%
Additional information: Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.
References: https://doi.org/10.1186/1471-2164-16-S13-S6
Cell line passport of iNV20
Catalogue number: NVPS00034
Name: iNV20
Description: american mink (Neovison vison) induced pluripotent stem cells derived from embryonic fibroblasts
Authors: Menzorov A.G., Matveeva N.M., Khabarova A.A.
Contamination analysis: bacteria, fungi and mycoplasma not detected
Karyotype: 2n=30, XY, chromosome number variability 29-32, tetraploid cells 21%, modal chromosome number 30
Pluripotency: pluripotency is shown byteratoma formation in SCID mice
Additional characteristics:
Species control: cytogenetic
Cryoconservation passage: 3
Area of application: study of pluripotency, developmental biology
Source
Species: Neogale vison
Tissue: fibroblasts
Date: 01.01.2015
Cell culture
Morphology: mink pluripotent stem cell colonies
Cell culture method: monolayer
Cell culture medium: a-MEM, ES cell qualified FBS 15%, NEAA 1%, Glutamine 1%, PenStrep 1%, 2-Mercaptoethanol 0.1 mM
Cell culture conditions: 37°C, 5% CO2
Passage protocol: passage with trypsin-EDTA 0.25%, split 1:3 — 1:6
Cryoconservation: 90% KSR, 10% DMSO
Cryoconservation cell concentration: 0.5 mln cells / ml
Cell viability after cryoconservation: 70%
Additional information: Plastic is coated with 0.1% gelatin. 13.5 dpc fibroblasts of ICR mouse strain are used as feeder cells.
References: https://doi.org/10.1186/1471-2164-16-S13-S6
Immortalised cell lines
Cell line passport of CHO-hCNTN6-HA
Catalogue number: CGOC00103
Name: CHO-hCNTN6-HA
Description: genetically modified CHO cell line (Chinese hamster ovary cells, Cricetulus griseus) with a constitutive expression of the human CNTN6 gene and HA-tag
Authors: Yunusova A.M., Chvileva A.S., Shnaider T.A.
Contamination analysis: bacteria, fungi and mycoplasma not detected
Karyotype: 2n = 22, chromosome number variability 16-20, modal chromosome number 19, polyploid cells 13%
Pluripotency:
Additional characteristics:
Species control: cytogenetic
Cryoconservation passage: 22
Area of application: Notch signaling pathway study
Source
Species: Cricetulus griseus
Tissue: ovary
Date: 09.10.2024
Cell culture
Morphology: epithelial-like cells
Cell culture method: monolayer
Cell culture medium: DMEM/F12, FBS 10%, PenStrep 1%
Cell culture conditions: 37°C, 5% CO2
Passage protocol: cell passage with 0.25% Trypsin-EDTA at the ratio 1:3 — 1:10
Cryoconservation: 50% FBS, 40% DMEM/F12, 10% DMSO
Cryoconservation cell concentration: 0.5 mln cells / ml
Cell viability after cryoconservation: 90%
Additional information:
References:
Cell line passport of CHO-hDLL1-HA
Catalogue number: CGOC00104
Name: CHO-hDLL1-HA
Description: genetically modified CHO cell line (Chinese hamster ovary cells, Cricetulus griseus) with a constitutive expression of the human DLL1 gene and HA-tag
Authors: Yunusova A.M., Chvileva A.S., Shnaider T.A.
Contamination analysis: bacteria, fungi and mycoplasma not detected
Karyotype: 2n = 22, chromosome number variability 19-22, modal chromosome number 20, polyploid cells 9%
Pluripotency:
Additional characteristics:
Species control: cytogenetic
Cryoconservation passage: 20
Area of application: Notch signaling pathway study
Source
Species: Cricetulus griseus
Tissue: ovary
Date: 09.10.2024
Cell culture
Morphology: epithelial-like cells
Cell culture method: monolayer
Cell culture medium: DMEM/F12, FBS 10%, PenStrep 1%
Cell culture conditions: 37°C, 5% CO2
Passage protocol: cell passage with 0.25% Trypsin-EDTA at the ratio 1:3 — 1:10
Cryoconservation: 50% FBS, 40% DMEM/F12, 10% DMSO
Cryoconservation cell concentration: 0.5 mln cells / ml
Cell viability after cryoconservation: 90%
Additional information:
References:
Cell line passport of CHO-hNOTCH1-FLAG
Catalogue number: CGOC00105
Name: CHO-hNOTCH1-FLAG
Description: genetically modified CHO cell line (Chinese hamster ovary cells, Cricetulus griseus) with a constitutive expression of the human NOTCH1 gene and FLAG-tag
Authors: Yunusova A.M., Chvileva A.S., Shnaider T.A.
Contamination analysis: bacteria, fungi and mycoplasma not detected
Karyotype: 2n = 22, chromosome number variability 18-21, modal chromosome number 20, polyploid cells 12%
Pluripotency:
Additional characteristics:
Species control: cytogenetic
Cryoconservation passage: 23
Area of application: Notch signaling pathway study
Source
Species: Cricetulus griseus
Tissue: ovary
Date: 09.10.2024
Cell culture
Morphology: epithelial-like cells
Cell culture method: monolayer
Cell culture medium: DMEM/F12, FBS 10%, PenStrep 1%
Cell culture conditions: 37°C, 5% CO2
Passage protocol: cell passage with 0.25% Trypsin-EDTA at the ratio 1:3 — 1:10
Cryoconservation: 50% FBS, 40% DMEM/F12, 10% DMSO
Cryoconservation cell concentration: 0.5 mln cells / ml
Cell viability after cryoconservation: 90%
Additional information:
References:
Cell line passport of CHO-hNOTCH2-FLAG
Catalogue number: CGOC00106
Name: CHO-hNOTCH2-FLAG
Description: genetically modified CHO cell line (Chinese hamster ovary cells, Cricetulus griseus) with a constitutive expression of the human NOTCH2 gene and FLAG-tag
Authors: Yunusova A.M., Chvileva A.S., Shnaider T.A.
Contamination analysis: bacteria, fungi and mycoplasma not detected
Karyotype: 2n = 22, chromosome number variability 20-21, modal chromosome number 21, polyploid cells 15%
Pluripotency:
Additional characteristics:
Species control: cytogenetic
Cryoconservation passage: 23
Area of application: Notch signaling pathway study
Source
Species: Cricetulus griseus
Tissue: ovary
Date: 09.10.2024
Cell culture
Morphology: epithelial-like cells
Cell culture method: monolayer
Cell culture medium: DMEM/F12, FBS 10%, PenStrep 1%
Cell culture conditions: 37°C, 5% CO2
Passage protocol: cell passage with 0.25% Trypsin-EDTA at the ratio 1:3 — 1:10
Cryoconservation: 50% FBS, 40% DMEM/F12, 10% DMSO
Cryoconservation cell concentration: 0.5 mln cells / ml
Cell viability after cryoconservation: 90%
Additional information:
References:
Bird fibroblasts
Cell line passport of OFC1A
Catalogue number: PMOF00097
Name: OFC1A
Description: Great tit ovary cells with fibroblast morphology
Authors: Pristyazhnyuk I.E., Malinovskaya L.P.
Contamination analysis: bacteria, fungi and mycoplasma not detected
Karyotype: 2N,ZW, 6 pairs of macrochromosomes, 33 pairs of microchromosomes
Pluripotency:
Additional characteristics: presence of rearranged chromosome homologues to chromosomes 4, 5 or 6.
Species control: cytogenetic
Cryoconservation passage: 8
Area of application:
Source
Species: Parus major
Tissue: ovary
Date: 26.08.2022
Cell culture
Morphology: fibroblast-like cells
Cell culture method: monolayer
Cell culture medium: DMEM, FBS 10%, 2% chicken serum, NEAA 1%, Glutamine 1%, PenStrep 1%
Cell culture conditions: 37°C, 5% CO2
Passage protocol: cell passage with Trypsin-EDTA at the ratio 1:3
Cryoconservation: 50% FBS, 40% DMEM, 10% DMSO
Cryoconservation cell concentration: 0.5 mln cells / ml
Cell viability after cryoconservation: 70%
Additional information:
References: https://doi.org/10.3390/ani12131724
Protists
Cell line passport of THAU1
Catalogue number: TAHE00071
Name: THAU1
Description: the protist Thraustochytrium aureum ssp. strugatskii was isolated from the dissociated comb jelly Beroe ovata (from the Black Sea)
Authors: Menzorov A.G., Doroshkov A.V.
Contamination analysis: bacteria, fungi and mycoplasma not detected
Karyotype:
Pluripotency:
Additional characteristics:
Species control: rRNA sequencing
Cryoconservation passage: 10
Area of application: biotechnology, fatty acid production, study of the Labyrinthulea life cycle
Source
Species: Thraustochytrium aureum ssp. strugatskii
Tissue:
Date: 26.11.2020
Cell culture
Morphology: “colonies” of cells
Cell culture method: monolayer
Cell culture medium: a) FAND culture medium: 17 ASW, 5% FBS,
5% DMEM (prepared from powder on 17‰ ASW), x0.05 NEAA, x1 PenStrep; b) 790 By+ (ATCC)
Cell culture conditions: room temperature
Passage protocol: manual passage (scraping and resuspending) at the ratio 1:10 — 1:100
Cryoconservation: 90% FBS, 10% DMSO
Cryoconservation cell concentration: 1 mln cells / ml
Cell viability after cryoconservation: 70%
Additional information:
References: http://dx.doi.org/10.7717/peerj.12737
Cell line passport of THCA1
Catalogue number: TAHE00107
Name: THCA1
Description: the protist Thraustochytrium caudivorum was isolated from the biota of the free-living flatworm Macrostomum lignano
Authors: Menzorov A.G., Biryukov M.Yu.
Contamination analysis: bacteria, fungi and mycoplasma not detected
Karyotype:
Pluripotency:
Additional characteristics:
Species control: rRNA sequencing (NCBI Genbank PV862890.1 and PV862891.1)
Cryoconservation passage: 12
Area of application: study of host-parasite interactions, study of the life cycle of Labyrinthulea
Source
Species: Thraustochytrium caudivorum
Tissue:
Date: 22.07.2025
Cell culture
Morphology: single cells
Cell culture method: monolayer
Cell culture medium: 790 By+ (ATCC)
Cell culture conditions: room temperature
Passage protocol: manual passage (scraping and resuspending) at the ratio 1:2 — 1:5
Cryoconservation: 90% FBS, 10% DMSO
Cryoconservation cell concentration: 1 mln cells / ml
Cell viability after cryoconservation: 70%
Additional information:
References:
Cell line passport of THKI1
Catalogue number: TAHE00108
Name: THKI1
Description: the protist Thraustochytrium kinnei was isolated from the dissociated comb jelly Beroe ovata (from the Black Sea)
Authors: Menzorov A.G., Doroshkov A.V.
Contamination analysis: bacteria, fungi and mycoplasma not detected
Karyotype:
Pluripotency:
Additional characteristics:
Species control: rRNA sequencing
Cryoconservation passage: 8
Area of application: biotechnology, fatty acid production, study of the Labyrinthulea life cycle
Source
Species: Thraustochytrium kinnei
Tissue:
Date: 13.02.2025
Cell culture
Morphology: “colonies” of cells
Cell culture method: monolayer
Cell culture medium: a) 790 By+ (ATCC); b) FAND culture medium: 17 ASW, 5% FBS,
5% DMEM (prepared from powder on 17‰ ASW), x0.05 NEAA, x1 PenStrep
Cell culture conditions: room temperature
Passage protocol: manual passage (scraping and resuspending) at the ratio 1:10 — 1:100
Cryoconservation: 90% FBS, 10% DMSO
Cryoconservation cell concentration: 1 mln cells / ml
Cell viability after cryoconservation: 70%
Additional information:
References: http://dx.doi.org/10.7717/peerj.12737